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Knockdown of lncRNA MEG8 inhibits cell proliferation and invasion, but promotes cell apoptosis in hemangioma, via miR‑203‑induced mediation of the Notch signaling pathway

  • Authors:
    • Zhenfeng Hu
    • Xiangmei Liu
    • Jing Guo
    • Lei Zhuo
    • Yongdong Chen
    • Haojun Yuan
  • View Affiliations / Copyright

    Affiliations: Department of General Surgery II (Department of Plastic Surgery), Affiliated Hospital of Hebei University of Engineering, Hebei, Handan 056002, P.R. China, Department of Plastic Surgery, Handan Seventh Hospital, Hebei, Handan 056001, P.R. China, Department of Cardiology, Handan Central Hospital, Hebei, Handan 056001, P.R. China, Department of General Surgery III, Handan First Hospital, Hebei, Handan 056002, P.R. China
    Copyright: © Hu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 872
    |
    Published online on: October 27, 2021
       https://doi.org/10.3892/mmr.2021.12512
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Abstract

As a member of the long non‑coding (lnc)RNA family, lncRNA maternally expressed 8, small nucleolar RNA host gene (MEG8), has been reported to serve an oncogenic role in several types of malignancies, including hepatocellular carcinoma, non‑small cell lung cancer and pancreatic cancer. The current study aimed to investigate the effect of the knockdown of MEG8 on human hemangioma endothelial cell (HemEC) proliferation, apoptosis and invasion, in addition to determining the underlying molecular mechanism. The knockdown of lncRNA MEG8 was achieved by transfecting lncRNA MEG8 small interfering (si)RNA into HemECs, while the combined knockdown of lncRNA MEG8 knockdown and microRNA (miR)‑203 was established by co‑transfecting lncRNA MEG8 siRNA and a miR‑203 inhibitor into HemECs. The cell proliferation, apoptosis and invasion and the expression levels of miR‑34a, miR‑200b, miR‑200b and Notch signaling pathway‑related factors were detected via CCK‑8 Kit, flow cytometry, Transwell, reverse transcription‑quantitative PCR and western blot assay, respectively. The knockdown of lncRNA MEG8 significantly inhibited proliferation (P<0.05) and invasion (P<0.05), but promoted apoptosis (P<0.01) in HemECs. Furthermore, lncRNA MEG8 knockdown upregulated miR‑203 (P<0.01) expression, but did not alter miR‑34a or miR‑200b expression (both P>0.05). Subsequent experiments revealed that miR‑203 silencing exerted no significant effect on the expression levels of lncRNA MEG8 (P>0.05) in HemECs. In addition, miR‑203 silencing increased cell proliferation (P<0.05) and invasion (P<0.01), but suppressed apoptosis (P<0.05). miR‑203 silencing also reversed the effect of lncRNA MEG8 knockdown on the proliferation (P<0.05), apoptosis (P<0.001) and invasion (P<0.01) of HemECs. Moreover, lncRNA MEG8 knockdown downregulated jagged canonical notch ligand 1 (JAG1; P<0.05) and Notch1 (P<0.05) expression levels, while miR‑203 silencing upregulated JAG1 (P<0.01) and Notch1 (P<0.01) expression levels and reversed the effects of lncRNA MEG8 knockdown on JAG1 (P<0.01) and Notch1 (P<0.01) expression in HemECs. In conclusion, the findings of the present study suggested that lncRNA MEG8 knockdown may inhibit cell proliferation and invasion, but promote cell apoptosis in hemangioma via miR‑203‑induced mediation of the Notch signaling pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Hu Z, Liu X, Guo J, Zhuo L, Chen Y and Yuan H: Knockdown of <em>lncRNA</em> <em>MEG8</em> inhibits cell proliferation and invasion, but promotes cell apoptosis in hemangioma, via <em>miR‑203</em>‑induced mediation of the Notch signaling pathway. Mol Med Rep 24: 872, 2021.
APA
Hu, Z., Liu, X., Guo, J., Zhuo, L., Chen, Y., & Yuan, H. (2021). Knockdown of <em>lncRNA</em> <em>MEG8</em> inhibits cell proliferation and invasion, but promotes cell apoptosis in hemangioma, via <em>miR‑203</em>‑induced mediation of the Notch signaling pathway. Molecular Medicine Reports, 24, 872. https://doi.org/10.3892/mmr.2021.12512
MLA
Hu, Z., Liu, X., Guo, J., Zhuo, L., Chen, Y., Yuan, H."Knockdown of <em>lncRNA</em> <em>MEG8</em> inhibits cell proliferation and invasion, but promotes cell apoptosis in hemangioma, via <em>miR‑203</em>‑induced mediation of the Notch signaling pathway". Molecular Medicine Reports 24.6 (2021): 872.
Chicago
Hu, Z., Liu, X., Guo, J., Zhuo, L., Chen, Y., Yuan, H."Knockdown of <em>lncRNA</em> <em>MEG8</em> inhibits cell proliferation and invasion, but promotes cell apoptosis in hemangioma, via <em>miR‑203</em>‑induced mediation of the Notch signaling pathway". Molecular Medicine Reports 24, no. 6 (2021): 872. https://doi.org/10.3892/mmr.2021.12512
Copy and paste a formatted citation
x
Spandidos Publications style
Hu Z, Liu X, Guo J, Zhuo L, Chen Y and Yuan H: Knockdown of <em>lncRNA</em> <em>MEG8</em> inhibits cell proliferation and invasion, but promotes cell apoptosis in hemangioma, via <em>miR‑203</em>‑induced mediation of the Notch signaling pathway. Mol Med Rep 24: 872, 2021.
APA
Hu, Z., Liu, X., Guo, J., Zhuo, L., Chen, Y., & Yuan, H. (2021). Knockdown of <em>lncRNA</em> <em>MEG8</em> inhibits cell proliferation and invasion, but promotes cell apoptosis in hemangioma, via <em>miR‑203</em>‑induced mediation of the Notch signaling pathway. Molecular Medicine Reports, 24, 872. https://doi.org/10.3892/mmr.2021.12512
MLA
Hu, Z., Liu, X., Guo, J., Zhuo, L., Chen, Y., Yuan, H."Knockdown of <em>lncRNA</em> <em>MEG8</em> inhibits cell proliferation and invasion, but promotes cell apoptosis in hemangioma, via <em>miR‑203</em>‑induced mediation of the Notch signaling pathway". Molecular Medicine Reports 24.6 (2021): 872.
Chicago
Hu, Z., Liu, X., Guo, J., Zhuo, L., Chen, Y., Yuan, H."Knockdown of <em>lncRNA</em> <em>MEG8</em> inhibits cell proliferation and invasion, but promotes cell apoptosis in hemangioma, via <em>miR‑203</em>‑induced mediation of the Notch signaling pathway". Molecular Medicine Reports 24, no. 6 (2021): 872. https://doi.org/10.3892/mmr.2021.12512
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