Open Access

Polydatin protects neuronal cells from hydrogen peroxide damage by activating CREB/Ngb signaling

  • Authors:
    • Huihui Zhang
    • Yadan Li
    • Yu Xun
    • Hui Liu
    • Chenxi Wei
    • Hao Wang
    • Xiaoping Yang
    • Shishan Yuan
    • Ning Liu
    • Shuanglin Xiang
  • View Affiliations

  • Published online on: November 8, 2021     https://doi.org/10.3892/mmr.2021.12525
  • Article Number: 9
  • Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Oxidative stress‑induced neuronal cell death contributes significantly to the physiological processes of a number of neurological disorders. Polydatin (PD) has been reported to protect against Alzheimer's disease (AD), ischemic stroke and traumatic brain injury. However, the underlying neuroprotective mechanisms remain to be elucidated. The current study suggested that PD activates AKT/cAMP response element‑binding protein (CREB) signaling and induces neuroglobin (Ngb) to protect neuronal cells from hydrogen peroxide (H2O2) in vitro. PD inhibited the H2O2‑induced neuronal cell death of primary mouse cortical neurons and N2a cells. Functional studies showed that PD attenuated H2O2‑induced mitochondrial dysfunction and mitochondrial reactive oxygen species production. Mechanistically, PD was verified to induce the phosphorylation of AKT and CREB and increase the protein level of Ngb. The luciferase assay results showed that Ngb transcriptional activity was activated by CREB, especially after PD treatment. It was further indicated that PD increased the transcription of Ngb by enhancing the binding of CREB to the promoter region of Ngb. Finally, Ngb knockdown largely attenuated the neuroprotective role of PD against H2O2. The results indicated that PD protected neuronal cells from H2O2 by activating CREB/Ngb signaling in neuronal cells, indicating that PD has a neuroprotective effect against neurodegenerative diseases.
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January-2022
Volume 25 Issue 1

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Spandidos Publications style
Zhang H, Li Y, Xun Y, Liu H, Wei C, Wang H, Yang X, Yuan S, Liu N, Xiang S, Xiang S, et al: Polydatin protects neuronal cells from hydrogen peroxide damage by activating CREB/Ngb signaling. Mol Med Rep 25: 9, 2022
APA
Zhang, H., Li, Y., Xun, Y., Liu, H., Wei, C., Wang, H. ... Xiang, S. (2022). Polydatin protects neuronal cells from hydrogen peroxide damage by activating CREB/Ngb signaling. Molecular Medicine Reports, 25, 9. https://doi.org/10.3892/mmr.2021.12525
MLA
Zhang, H., Li, Y., Xun, Y., Liu, H., Wei, C., Wang, H., Yang, X., Yuan, S., Liu, N., Xiang, S."Polydatin protects neuronal cells from hydrogen peroxide damage by activating CREB/Ngb signaling". Molecular Medicine Reports 25.1 (2022): 9.
Chicago
Zhang, H., Li, Y., Xun, Y., Liu, H., Wei, C., Wang, H., Yang, X., Yuan, S., Liu, N., Xiang, S."Polydatin protects neuronal cells from hydrogen peroxide damage by activating CREB/Ngb signaling". Molecular Medicine Reports 25, no. 1 (2022): 9. https://doi.org/10.3892/mmr.2021.12525