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CMTM5 inhibits the development of prostate cancer via the EGFR/PI3K/AKT signaling pathway

  • Authors:
    • Linjin Li
    • Yiren Hu
    • Dake Chen
    • Jianlong Zhu
    • Wenshuo Bao
    • Xiaomin Xu
    • Heyi Chen
    • Wu Chen
    • Rui Feng
  • View Affiliations / Copyright

    Affiliations: Department of Urology, The Third Clinical Institute Affiliated to Wenzhou Medical University, The Third Affiliated Hospital of Shanghai University, Wenzhou People's Hospital, Wenzhou, Zhejiang 325000, P.R. China, Department of Urology, Zhenjiang Hospital of Chinese Traditional and Western Medicine, Zhenjiang, Jiangsu 212002, P.R. China
    Copyright: © Li et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 17
    |
    Published online on: November 15, 2021
       https://doi.org/10.3892/mmr.2021.12533
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Abstract

Prostate cancer (PCa) endangers the life and health of older men. Most PCa cases develop into castration‑resistant PCa (CRPC) within 2 years. At present, the molecular mechanisms of the occurrence and development of PCa and its transformation to CRPC remain unknown. The present study aimed to investigate the role of CKLF‑like Marvel transmembrane domain containing family member 5 (CMTM5) in PCa and its molecular mechanism in vitro. PCa tissues and paired adjacent normal prostate tissues from 70 patients were collected to examine the expression levels of CMTM5 and EGFR via immunohistochemistry, reverse transcription‑quantitative PCR and western blotting. Then, CMTM5‑overexpressing DU145 cells were constructed, and CMTM5 expression in these transfected cells and vector control cells was examined via western blotting. Cell Counting Kit‑8 and plate clone formation assays were used to evaluate the proliferation and colony number of CMTM5‑overexpressing cells and vector control cells. Then, cell migration and invasion were assessed using wound healing assay, Transwell assay and immunofluorescence analysis with DAPI staining. The effect of CMTM5 on apoptosis and its underlying molecular mechanism were examined using western blotting and flow cytometry. The results demonstrated that CMTM5 expression in PCa tissues and cell lines was significantly downregulated, while EFGR expression was significantly upregulated. The proportion of high CMTM5 expression in PCa tissues was significantly lower compared with that in normal prostate tissues. By contrast, the proportion of high EGFR expression in PCa tissues was significantly increased compared with that in normal prostate tissues. Moreover, CMTM5 overexpression significantly inhibited cell proliferation, migration and invasion, and promoted cell apoptosis compared with vector control cells in vitro. Furthermore, the regulation of PCa by CMTM5 was associated with the downregulation of PI3K/AKT and its downstream Bcl‑2 expression, as well as the upregulation of Bax expression. In conclusion, CMTM5 may be an effective tumor suppressor gene for PCa, especially for castration‑resistant PCa, by downregulating EGFR and PI3K/AKT signaling pathway components.
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Copy and paste a formatted citation
Spandidos Publications style
Li L, Hu Y, Chen D, Zhu J, Bao W, Xu X, Chen H, Chen W and Feng R: CMTM5 inhibits the development of prostate cancer via the EGFR/PI3K/AKT signaling pathway. Mol Med Rep 25: 17, 2022.
APA
Li, L., Hu, Y., Chen, D., Zhu, J., Bao, W., Xu, X. ... Feng, R. (2022). CMTM5 inhibits the development of prostate cancer via the EGFR/PI3K/AKT signaling pathway. Molecular Medicine Reports, 25, 17. https://doi.org/10.3892/mmr.2021.12533
MLA
Li, L., Hu, Y., Chen, D., Zhu, J., Bao, W., Xu, X., Chen, H., Chen, W., Feng, R."CMTM5 inhibits the development of prostate cancer via the EGFR/PI3K/AKT signaling pathway". Molecular Medicine Reports 25.1 (2022): 17.
Chicago
Li, L., Hu, Y., Chen, D., Zhu, J., Bao, W., Xu, X., Chen, H., Chen, W., Feng, R."CMTM5 inhibits the development of prostate cancer via the EGFR/PI3K/AKT signaling pathway". Molecular Medicine Reports 25, no. 1 (2022): 17. https://doi.org/10.3892/mmr.2021.12533
Copy and paste a formatted citation
x
Spandidos Publications style
Li L, Hu Y, Chen D, Zhu J, Bao W, Xu X, Chen H, Chen W and Feng R: CMTM5 inhibits the development of prostate cancer via the EGFR/PI3K/AKT signaling pathway. Mol Med Rep 25: 17, 2022.
APA
Li, L., Hu, Y., Chen, D., Zhu, J., Bao, W., Xu, X. ... Feng, R. (2022). CMTM5 inhibits the development of prostate cancer via the EGFR/PI3K/AKT signaling pathway. Molecular Medicine Reports, 25, 17. https://doi.org/10.3892/mmr.2021.12533
MLA
Li, L., Hu, Y., Chen, D., Zhu, J., Bao, W., Xu, X., Chen, H., Chen, W., Feng, R."CMTM5 inhibits the development of prostate cancer via the EGFR/PI3K/AKT signaling pathway". Molecular Medicine Reports 25.1 (2022): 17.
Chicago
Li, L., Hu, Y., Chen, D., Zhu, J., Bao, W., Xu, X., Chen, H., Chen, W., Feng, R."CMTM5 inhibits the development of prostate cancer via the EGFR/PI3K/AKT signaling pathway". Molecular Medicine Reports 25, no. 1 (2022): 17. https://doi.org/10.3892/mmr.2021.12533
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