Open Access

Effect of therapeutic hypothermia against renal injury in a rat model of asphyxial cardiac arrest: Α focus on the survival rate, pathophysiology and antioxidant enzymes

  • Authors:
    • So Eun Kim
    • Ha-Young Shin
    • Eui-Yong Lee
    • Yeo-Jin Yoo
    • Ryun-Hee Kim
    • Jeong-Hwi Cho
    • Tae-Kyeong Lee
    • Dongchoon Ahn
    • Byung-Yong Park
    • Jae Chol Yoon
    • Seongkweon Hong
    • In-Shik Kim
    • Hyun-Jin Tae
    • Moo-Ho Won
  • View Affiliations

  • Published online on: November 18, 2021     https://doi.org/10.3892/mmr.2021.12535
  • Article Number: 19
  • Copyright: © Kim et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Although multi‑organ dysfunction is associated with the survival rate following cardiac arrest (CA), the majority of studies to date have focused on hearts and brains, and few studies have considered renal failure. The objective of the present study, therefore, was to examine the effects of therapeutic hypothermia on the survival rate, pathophysiology and antioxidant enzymes in rat kidneys following asphyxial CA. Rats were sacrificed one day following CA. The survival rate, which was estimated using Kaplan‑Meier analysis, was 42.9% one day following CA. However, hypothermia, which was induced following CA, significantly increased the survival rate (71.4%). In normothermia rats with CA, the serum blood urea nitrogen level was significantly increased one day post‑CA. In addition, the serum creatinine level was significantly increased one day post‑CA. However, in CA rats exposed to hypothermia, the levels of urea nitrogen and creatinine significantly decreased following CA. Histochemical staining revealed a significant temporal increase in renal injury after the normothermia group was subjected to CA. However, renal injury was significantly decreased in the hypothermia group. Immunohistochemical analysis of the kidney revealed a significant decrease in antioxidant enzymes (copper‑zinc superoxide dismutase, manganese superoxide dismutase, glutathione peroxidase and catalase) with time in the normothermia group. However, in the hypothermia group, these enzymes were significantly elevated following CA. Collectively, the results revealed that renal dysfunction following asphyxial CA was strongly associated with the early survival rate and therapeutic hypothermia reduced renal injury via effective antioxidant mechanisms.
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January-2022
Volume 25 Issue 1

Print ISSN: 1791-2997
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Spandidos Publications style
Kim SE, Shin H, Lee E, Yoo Y, Kim R, Cho J, Lee T, Ahn D, Park B, Yoon JC, Yoon JC, et al: Effect of therapeutic hypothermia against renal injury in a rat model of asphyxial cardiac arrest: Α focus on the survival rate, pathophysiology and antioxidant enzymes. Mol Med Rep 25: 19, 2022
APA
Kim, S.E., Shin, H., Lee, E., Yoo, Y., Kim, R., Cho, J. ... Won, M. (2022). Effect of therapeutic hypothermia against renal injury in a rat model of asphyxial cardiac arrest: Α focus on the survival rate, pathophysiology and antioxidant enzymes. Molecular Medicine Reports, 25, 19. https://doi.org/10.3892/mmr.2021.12535
MLA
Kim, S. E., Shin, H., Lee, E., Yoo, Y., Kim, R., Cho, J., Lee, T., Ahn, D., Park, B., Yoon, J. C., Hong, S., Kim, I., Tae, H., Won, M."Effect of therapeutic hypothermia against renal injury in a rat model of asphyxial cardiac arrest: Α focus on the survival rate, pathophysiology and antioxidant enzymes". Molecular Medicine Reports 25.1 (2022): 19.
Chicago
Kim, S. E., Shin, H., Lee, E., Yoo, Y., Kim, R., Cho, J., Lee, T., Ahn, D., Park, B., Yoon, J. C., Hong, S., Kim, I., Tae, H., Won, M."Effect of therapeutic hypothermia against renal injury in a rat model of asphyxial cardiac arrest: Α focus on the survival rate, pathophysiology and antioxidant enzymes". Molecular Medicine Reports 25, no. 1 (2022): 19. https://doi.org/10.3892/mmr.2021.12535