MicroRNA‑671‑5p inhibits cell proliferation, migration and invasion in non‑small cell lung cancer by targeting MFAP3L Retraction in /10.3892/mmr.2024.13225

Ye,Junhui; Luo,Wujun; Luo,Lingling; Zhai,Limin; Huang,Pingping

doi:10.3892/mmr.2021.12546

MicroRNA‑671‑5p inhibits cell proliferation, migration and invasion in non‑small cell lung cancer by targeting MFAP3L

Retraction in: /10.3892/mmr.2024.13225

Authors:
- Junhui Ye
- Wujun Luo
- Lingling Luo
- Limin Zhai
- Pingping Huang
View Affiliations

Affiliations: Department of Respiratory Medicine, The People's Hospital of Sanmen, Taizhou, Zhejiang 317100, P.R. China
Published online on: November 28, 2021 https://doi.org/10.3892/mmr.2021.12546
Article Number: 30
Copyright: © Ye et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

MicroRNA (miR)‑671‑5p serves as a tumor suppressor in several types of cancer, including gastric and breast cancer. However, the function of miR‑671‑5p in non‑small cell lung cancer (NSCLC) has not been described in detail. The present study aimed to investigate the role of miR‑671‑5p in NSCLC. The expression levels of miR‑671‑5p were determined in NSCLC tissue samples and cell lines using reverse transcription‑quantitative PCR. Prediction of miR‑671‑5p targets was performed using the TargetScan database and verified by luciferase reporter assay and western blot analysis. Functional experiments, including Cell Counting Kit‑8, wound healing and Transwell assays, were performed in NSCLC cells. The results of the present study demonstrated that lower expression levels of miR‑671‑5p were observed in NSCLC tissues and cell lines compared with those in the corresponding controls. Low miR‑671‑5p levels were significantly associated with an advanced Tumor‑Node‑Metastasis stage and lymph node metastasis in patients with NSCLC. Microfibril‑associated protein 3‑like (MFAP3L) was confirmed to be a direct target of miR‑671‑5p. The proliferative, migratory and invasive abilities of NSCLC cells were suppressed following transfection with miR‑671‑5p mimics and promoted by the miR‑671‑5p inhibitor compared with those in the respective control groups. In addition, the effects of miR‑671‑5p on cell proliferation, migration and invasion, as well as the expression levels of proliferating cell nuclear antigen, E‑cadherin, N‑cadherin and vimentin were reversed by MFAP3L overexpression. In conclusion, targeting the miR‑671‑5p/MFAP3L signaling pathway may be a promising therapeutic strategy for NSCLC treatment.

View Figures

View References

1	Qin H, Wang F, Liu H, Zeng Z, Wang S, Pan X and Gao H: New advances in immunotherapy for non-small cell lung cancer. Am J Transl Res. 10:2234–2245. 2018.PubMed/NCBI
2	Wood SL, Pernemalm M, Crosbie PA and Whetton AD: Molecular histology of lung cancer: From targets to treatments. Cancer Treat Rev. 41:361–375. 2015. View Article : Google Scholar : PubMed/NCBI
3	Molina JR, Yang P, Cassivi SD, Schild SE and Adjei AA: Non-small cell lung cancer: Epidemiology, risk factors, treatment, and survivorship. Mayo Clin Proc. 83:584–594. 2008. View Article : Google Scholar : PubMed/NCBI
4	Lewis BP, Burge CB and Bartel DP: Conserved seed pairing, often flanked by adenosines, indicates that thousands of human genes are microRNA targets. Cell. 120:15–20. 2005. View Article : Google Scholar : PubMed/NCBI
5	Bartel DP: MicroRNAs: Genomics, biogenesis, mechanism, and function. Cell. 116:281–297. 2004. View Article : Google Scholar : PubMed/NCBI
6	Calin GA and Croce CM: MicroRNA signatures in human cancers. Nat Rev Cancer. 6:857–866. 2006. View Article : Google Scholar : PubMed/NCBI
7	Macfarlane LA and Murphy PR: MicroRNA: Biogenesis, function and role in cancer. Curr Genomics. 11:537–561. 2010. View Article : Google Scholar : PubMed/NCBI
8	Bartels CL and Tsongalis GJ: MicroRNAs: Novel biomarkers for human cancer. Clin Chem. 55:623–631. 2009. View Article : Google Scholar : PubMed/NCBI
9	Tan X, Li Z, Ren S, Rezaei K, Pan Q, Goldstein AT, Macri CJ, Cao D, Brem RF and Fu SW: Dynamically decreased miR-671-5p expression is associated with oncogenic transformation and radiochemoresistance in breast cancer. Breast Cancer Res. 21:892019. View Article : Google Scholar : PubMed/NCBI
10	Tan X, Fu Y, Chen L, Lee W, Lai Y, Rezaei K, Tabbara S, Latham P, Teal CB, Man YG, et al: miR-671-5p inhibits epithelial-to-mesenchymal transition by downregulating FOXM1 expression in breast cancer. Oncotarget. 7:293–307. 2016. View Article : Google Scholar : PubMed/NCBI
11	Li X, Nie C, Tian B, Tan X, Han W, Wang J, Jin Y, Li Y, Guan X, Hong A, et al: miR-671-5p Blocks the progression of human esophageal squamous cell carcinoma by suppressing FGFR2. Int J Biol Sci. 15:1892–1904. 2019. View Article : Google Scholar : PubMed/NCBI
12	Xin C, Lu S, Li Y, Zhang Y, Tian J, Zhang S, Yang S, Gao T and Xu J: miR-671-5p inhibits tumor proliferation by blocking cell cycle in osteosarcoma. DNA Cell Biol. 38:996–1004. 2019. View Article : Google Scholar : PubMed/NCBI
13	Chi XG, Meng XX, Ding DL, Xuan XH, Chen YZ, Cai Q and Wang A: HMGA1-mediated miR-671-5p targets APC to promote metastasis of clear cell renal cell carcinoma through Wnt signaling. Neoplasma. 67:46–53. 2020. View Article : Google Scholar : PubMed/NCBI
14	Li X and Diao H: Circular RNA circ_0001946 acts as a competing endogenous RNA to inhibit glioblastoma progression by modulating miR-671-5p and CDR1. J Cell Physiol. 234:13807–13819. 2019. View Article : Google Scholar : PubMed/NCBI
15	Albig AR, Becenti DJ, Roy TG and Schiemann WP: Microfibril-associate glycoprotein-2 (MAGP-2) promotes angiogenic cell sprouting by blocking notch signaling in endothelial cells. Microvasc Res. 76:7–14. 2008. View Article : Google Scholar : PubMed/NCBI
16	Mok SC, Bonome T, Vathipadiekal V, Bell A, Johnson ME, Wong KK, Park DC, Hao K, Yip DK, Donninger H, et al: A gene signature predictive for outcome in advanced ovarian cancer identifies a survival factor: Microfibril-associated glycoprotein 2. Cancer Cell. 16:521–532. 2009. View Article : Google Scholar : PubMed/NCBI
17	Spivey KA and Banyard J: A prognostic gene signature in advanced ovarian cancer reveals a microfibril-associated protein (MAGP2) as a promoter of tumor cell survival and angiogenesis. Cell Adhes Migr. 4:169–171. 2010. View Article : Google Scholar
18	Xiao J, Yin L, Li J, Zu H, Zhou Z, Zhao B and Sha J: Molecular cloning, identification and characteristics of NYD-SP9: Gene coding protein kinase presumably involved in spermatogenesis. Chin Sci Bull. 47:896–901. 2002. View Article : Google Scholar
19	Wu JH, Tian XY and Hao CY: The significance of a group of molecular markers and clinicopathological factors in identifying colorectal liver metastasis. Hepatogastroenterology. 58:1182–1188. 2011. View Article : Google Scholar : PubMed/NCBI
20	Lou X, Kang B, Zhang J, Hao C, Tian X, Li W, Xu N, Lu Y and Liu S: MFAP3L activation promotes colorectal cancer cell invasion and metastasis. Biochim Biophys Acta. 1842:1423–1432. 2014. View Article : Google Scholar : PubMed/NCBI
21	Wang J, Wu N, Zheng Q, Feng Y, Yan S, Lv C, Li S, Wang Y and Yang Y: Evaluation of the 7th edition of the TNM classification for lung cancer at a single institution. J Cancer Res Clin Oncol. 140:1189–1195. 2014. View Article : Google Scholar : PubMed/NCBI
22	Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(−ΔΔC(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI
23	Chen X, Zhang C and Wang X: Long noncoding RNA DLEU1 aggravates osteosarcoma carcinogenesis via regulating the miR-671-5p/DDX5 axis. Artif Cells Nanomed Biotechnol. 47:3322–3328. 2019. View Article : Google Scholar : PubMed/NCBI
24	Qiu T, Wang K, Li X and Jin J: miR-671-5p inhibits gastric cancer cell proliferation and promotes cell apoptosis by targeting URGCP. Exp Ther Med. 16:4753–4758. 2018.PubMed/NCBI
25	Song H, Xu Y, Xu T, Fan R, Jiang T, Cao M, Shi L and Song J: CircPIP5K1A activates KRT80 and PI3K/AKT pathway to promote gastric cancer development through sponging miR-671-5p. Biomed Pharmacother. 126:1099412020. View Article : Google Scholar : PubMed/NCBI
26	Jin W, Shi J and Liu M: Overexpression of miR-671-5p indicates a poor prognosis in colon cancer and accelerates proliferation, migration, and invasion of colon cancer cells. OncoTargets Ther. 12:6865–6873. 2019. View Article : Google Scholar : PubMed/NCBI
27	Ma C, Nie ZK, Guo HM and Kong Y: MiR-671-5p plays a promising role in restraining osteosarcoma cell characteristics through targeting TUFT1. J Biochem Mol Toxicol. 34:e224902020. View Article : Google Scholar : PubMed/NCBI
28	Zhang B: Guizhi Fuling pills inhibit the proliferation, migration and invasion of human cutaneous malignant melanoma cells by regulating the molecular axis of LncRNA TPT1-AS1/miR-671-5p. Cell Mol Biol (Noisy-le-grand). 66:148–154. 2020. View Article : Google Scholar : PubMed/NCBI
29	Savci-Heijink CD, Halfwerk H, Koster J and van de Vijver MJ: A novel gene expression signature for bone metastasis in breast carcinomas. Breast Cancer Res Treat. 156:249–259. 2016. View Article : Google Scholar : PubMed/NCBI