Open Access

Rapid detection of FMO3 single nucleotide polymorphisms using a pyrosequencing method

  • Authors:
    • Jin-Woo Park
    • In-Hwan Park
    • Jong-Min Kim
    • Ji Hyeon Noh
    • Kyoung-Ah Kim
    • Ji-Young Park
  • View Affiliations

  • Published online on: December 8, 2021     https://doi.org/10.3892/mmr.2021.12564
  • Article Number: 48
  • Copyright: © Park et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

The present study aimed to develop a reliable pyrosequencing method to detect four single nucleotide polymorphisms (SNPs) of the flavin‑containing monooxygenase 3 (FMO3) gene and to compare the ethnic differences in their allelic frequencies. The pyrosequencing method was used to detect four FMO3 SNPs, namely, c.855C>T (N285N, rs909530), c.441C>T (S147S, rs1800822), c.923A>G (E308G, rs2266780) and c.472G>A (E158K, rs2266782). The allelic frequencies of these SNPs in 122 unrelated Korean subjects were as follows: i) 44.7% for c.855C>T; ii) 23.4% for c.441C>T; iii) 23.0% for c.923A>G; and iv) 27.1% for c.472G>A. Linkage disequilibrium (LD) analysis revealed that the SNPs c.923A>G and c.472G>A exhibited a strong LD (D'=0.8289, r2=0.5332). In conclusion, the pyrosequencing method developed in this study was successfully applied to detect the c.855C>T, c.441C>T, c.923A>G and c.472G>A SNPs of FMO3.
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February-2022
Volume 25 Issue 2

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Park J, Park I, Kim J, Noh JH, Kim K and Park J: Rapid detection of <em>FMO3</em> single nucleotide polymorphisms using a pyrosequencing method. Mol Med Rep 25: 48, 2022
APA
Park, J., Park, I., Kim, J., Noh, J.H., Kim, K., & Park, J. (2022). Rapid detection of <em>FMO3</em> single nucleotide polymorphisms using a pyrosequencing method. Molecular Medicine Reports, 25, 48. https://doi.org/10.3892/mmr.2021.12564
MLA
Park, J., Park, I., Kim, J., Noh, J. H., Kim, K., Park, J."Rapid detection of <em>FMO3</em> single nucleotide polymorphisms using a pyrosequencing method". Molecular Medicine Reports 25.2 (2022): 48.
Chicago
Park, J., Park, I., Kim, J., Noh, J. H., Kim, K., Park, J."Rapid detection of <em>FMO3</em> single nucleotide polymorphisms using a pyrosequencing method". Molecular Medicine Reports 25, no. 2 (2022): 48. https://doi.org/10.3892/mmr.2021.12564