Cellular response of lung fibroblasts and epithelial cells to particulate matter<sub>10</sub> treatment examined via comparative transcriptome analysis

Park,Kunhyang; Kim,Kwangho; Ryu,Tae Young; Lee,Jinkwon; Lee,Moo-Seung; Son,Mi-Young; Lee,Seon-Jin; Park,Young-Jun; Cho,Hyun-Soo; Kim,Dae-Soo

doi:10.3892/mmr.2022.12598

Cellular response of lung fibroblasts and epithelial cells to particulate matter₁₀ treatment examined via comparative transcriptome analysis

Authors:
- Kunhyang Park
- Kwangho Kim
- Tae Young Ryu
- Jinkwon Lee
- Moo-Seung Lee
- Mi-Young Son
- Seon-Jin Lee
- Young-Jun Park
- Hyun-Soo Cho
- Dae-Soo Kim
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Affiliations: Environmental Diseases Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 34141, Republic of Korea
Published online on: January 13, 2022 https://doi.org/10.3892/mmr.2022.12598
Article Number: 82

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Abstract

Particulate matter (PM) can be categorized by particle size (PM₁₀, PM_2.5 and PM_1.0), which is an important factor affecting the biological response. Exposure to PM in the air (dust, smoke, dirt and biological contaminants) is clearly associated with lung disease (lung cancer, pneumonia and asthma). Although PM primarily affects lung epithelial cells, the specific response of related cell types to PM remains to be elucidated. The present study performed Gene Ontology (GO) analysis programs (Clustering GO and Database for Annotation, Visualization and Integrated Discovery) on differentially expressed genes in lung epithelial cells (WI‑38 VA‑13) and fibroblasts (WI‑38) following treatment with PM₁₀ and evaluated the cell‑specific biological responses related to cell proliferation, apoptosis, adhesion and extracellular matrix production. The results suggested that short‑ or long‑term exposure to PM may affect cell condition and may consequently be related to several human diseases, including lung cancer and cardiopulmonary disease.

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