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Total glucosides of paeony ameliorates oxidative stress, apoptosis and inflammatory response by regulating the Smad7‑TGF‑β pathway in allergic rhinitis

  • Authors:
    • Yangzi Jin
    • Aichun Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Otolaryngology, The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, Zhejiang 310000, P.R. China
    Copyright: © Jin et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 83
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    Published online on: January 13, 2022
       https://doi.org/10.3892/mmr.2022.12599
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Abstract

Total glucosides of paeony (TGP), an active ingredient extracted from the root of Paeonia alba, has been reported to display an anti‑inflammatory effect. However, the effect of TGP on allergic rhinitis (AR) is still unknown. The present study aimed to assess the role of TGP in an AR mouse model. An AR mouse model was established using the ovalbumin method. The expression levels of Smad7/TGF‑β pathway‑related prtoeins in nasal mucosa tissues were determined by immunofluorescence, immunohistochemistry and western blotting. The severity of nasal allergic symptoms was detected by recording the frequency of sneezing and nose rubbing motions in all mice for 20 min. The levels of IgE and inflammatory cytokines, including IL‑4, IL‑5, IL‑17 and IFN‑γ, in the serum were measured by conducting ELISAs. H&E staining, periodic acid‑Schiff staining and Masson staining were used to detected histopathological changes in mice. The concentrations of malondialdehyde and glutathione, and the activities of superoxide dismutase and catalase in tissue supernatant and serum were quantified using commercial assay kits. Apoptosis of nasal tissue cells was detected by performing TUNEL assays and western blotting. The expression of Smad7 was upregulated and that of TGF‑β was downregulated in the nasal tissue of AR mice. Additionally, TGP regulated the Smad7/TGF‑β pathway in the nasal tissue of AR mice. TGP alleviated serum IgE, nasal symptoms and histopathological changes in AR mice. Moreover, TGP ameliorated oxidative stress, cell apoptosis and inflammatory response. Smad7 small interfering RNA intervention aggravated the symptoms of AR mice via activation of the TGF‑β pathway and reversed the protective effect of TGP in AR mice. TGP ameliorated oxidative stress, apoptosis and inflammatory response via the Smad7/TGF‑β pathway in AR.
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Copy and paste a formatted citation
Spandidos Publications style
Jin Y and Zhang A: Total glucosides of paeony ameliorates oxidative stress, apoptosis and inflammatory response by regulating the Smad7‑TGF‑β pathway in allergic rhinitis. Mol Med Rep 25: 83, 2022.
APA
Jin, Y., & Zhang, A. (2022). Total glucosides of paeony ameliorates oxidative stress, apoptosis and inflammatory response by regulating the Smad7‑TGF‑β pathway in allergic rhinitis. Molecular Medicine Reports, 25, 83. https://doi.org/10.3892/mmr.2022.12599
MLA
Jin, Y., Zhang, A."Total glucosides of paeony ameliorates oxidative stress, apoptosis and inflammatory response by regulating the Smad7‑TGF‑β pathway in allergic rhinitis". Molecular Medicine Reports 25.3 (2022): 83.
Chicago
Jin, Y., Zhang, A."Total glucosides of paeony ameliorates oxidative stress, apoptosis and inflammatory response by regulating the Smad7‑TGF‑β pathway in allergic rhinitis". Molecular Medicine Reports 25, no. 3 (2022): 83. https://doi.org/10.3892/mmr.2022.12599
Copy and paste a formatted citation
x
Spandidos Publications style
Jin Y and Zhang A: Total glucosides of paeony ameliorates oxidative stress, apoptosis and inflammatory response by regulating the Smad7‑TGF‑β pathway in allergic rhinitis. Mol Med Rep 25: 83, 2022.
APA
Jin, Y., & Zhang, A. (2022). Total glucosides of paeony ameliorates oxidative stress, apoptosis and inflammatory response by regulating the Smad7‑TGF‑β pathway in allergic rhinitis. Molecular Medicine Reports, 25, 83. https://doi.org/10.3892/mmr.2022.12599
MLA
Jin, Y., Zhang, A."Total glucosides of paeony ameliorates oxidative stress, apoptosis and inflammatory response by regulating the Smad7‑TGF‑β pathway in allergic rhinitis". Molecular Medicine Reports 25.3 (2022): 83.
Chicago
Jin, Y., Zhang, A."Total glucosides of paeony ameliorates oxidative stress, apoptosis and inflammatory response by regulating the Smad7‑TGF‑β pathway in allergic rhinitis". Molecular Medicine Reports 25, no. 3 (2022): 83. https://doi.org/10.3892/mmr.2022.12599
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