Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
March-2022 Volume 25 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
March-2022 Volume 25 Issue 3

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article Open Access

CpG site hypomethylation at ETS1‑binding region regulates DLK1 expression in Chinese patients with Tetralogy of Fallot

  • Authors:
    • Guixiang Tian
    • Lili He
    • Ruoyi Gu
    • Jingwei Sun
    • Weicheng Chen
    • Yanyan Qian
    • Xiaojing Ma
    • Weili Yan
    • Zhenshan Zhao
    • Ziqing Xu
    • Meijiao Suo
    • Wei Sheng
    • Guoying Huang
  • View Affiliations / Copyright

    Affiliations: Department of Ultrasound, Cardiovascular Center, Pediatrics Research Institute, National Children's Medical Center, Children's Hospital of Fudan University, Shanghai 201102, P.R. China, Pediatrics Department, Bengbu First People's Hospital, Bengbu, Anhui 233000, P.R. China
    Copyright: © Tian et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 93
    |
    Published online on: January 20, 2022
       https://doi.org/10.3892/mmr.2022.12609
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Tetralogy of Fallot (TOF) is the most common cyanotic congenital heart malformation accounting for ~10% of cases. Although the pathogenesis of TOF is complex and largely unknown, epigenetics plays a huge role, specifically DNA methylation. The protein δ like non‑canonical Notch ligand 1 (DLK1) gene encodes a non‑canonical ligand of the Notch signaling pathway, which is involved in heart development. However, the epigenetic mechanism of DLK1 in the pathogenesis of TOF is yet to be elucidated. Therefore, the present study aimed to clarify its specific mechanism. In this study, immunohistochemistry was used to detect the protein expression of DLK1 and the methylation status of the DLK1 promoter was measured via bisulfite sequencing PCR. Dual‑luciferase reporter assays were performed to examine the influence of transcription factor ETS proto‑oncogene 1 (ETS1) on DLK1 gene expression. The electrophoretic mobility shift assay and chromatin immunoprecipitation assay, both in vivo and in vitro, were used to verify the binding of the ETS1 transcription factor to the DLK1 promoter as well as the influence of methylation status of DLK1 promoter on this binding affinity. The expression of DLK1 in the right ventricular outflow tract was significantly lower in patients with Tetralogy of Fallot (TOF) than that in controls (P<0.001). Moreover, the methylation level of CpG site 10 and CpG site 11 in the DLK1_R region was significantly decreased in TOF cases compared with controls (P<0.01). The integral methylation levels of DLK1_R and the methylation status of the CpG site 11 were both positively associated with DLK1 protein expression in TOF cases. ETS1 was found to inhibit DLK1 transcriptional activity by binding to the CpG site 11 and this affinity could be influenced by the methylation level of the DLK1 promoter. These findings demonstrated that the hypomethylation of the DLK1 promoter could increase the binding affinity of ETS1 transcription factor, which in turn inhibited DLK1 gene transcriptional activity and contributed to the development of TOF.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

View References

1 

Apitz C, Webb GD and Redington AN: Tetralogy of Fallot. Lancet. 374:1462–1471. 2009. View Article : Google Scholar : PubMed/NCBI

2 

Page DJ, Miossec MJ, Williams SG, Monaghan RM, Fotiou E, Cordell HJ, Sutcliffe L, Topf A, Bourgey M, Bourque G, et al: Whole exome sequencing reveals the major genetic contributors to Nonsyndromic Tetralogy of Fallot. Circ Res. 124:553–563. 2019. View Article : Google Scholar : PubMed/NCBI

3 

Grunert M, Dorn C, Cui H, Dunkel I, Schulz K, Schoenhals S, Sun W, Berger F, Chen W and Sperling SR: Comparative DNA methylation and gene expression analysis identifies novel genes for structural congenital heart diseases. Cardiovasc Res. 112:464–477. 2016. View Article : Google Scholar : PubMed/NCBI

4 

Finn J, Sottoriva K, Pajcini KV, Kitajewski JK, Chen C, Zhang W, Malik AB and Liu Y: Dlk1-Mediated Temporal Regulation of Notch Signaling is required for differentiation of Alveolar Type II to Type I cells during repair. Cell Rep. 26:2942–2954.e5. 2019. View Article : Google Scholar : PubMed/NCBI

5 

de la Pompa JL and Epstein JA: Coordinating tissue interactions: Notch signaling in cardiac development and disease. Dev Cell. 22:244–254. 2012. View Article : Google Scholar : PubMed/NCBI

6 

McCright B, Lozier J and Gridley T: A mouse model of Alagille syndrome: Notch2 as a genetic modifier of Jag1 haploinsufficiency. Development. 129:1075–1082. 2002. View Article : Google Scholar : PubMed/NCBI

7 

Digilio MC, Luca AD, Lepri F, Guida V, Ferese R, Dentici ML, Angioni A, Marino B and Dallapiccola B: JAG1 mutation in a patient with deletion 22q11.2 syndrome and tetralogy of Fallot. Am J Med Genet A. 161A:3133–3136. 2013. View Article : Google Scholar : PubMed/NCBI

8 

Garg V: Notch signaling in aortic valve development and disease, Etiology and Morphogenesis of Congenital Heart Disease: From Gene Function and Cellular Interaction to Morphology. Nakanishi T, Markwald RR, Baldwin HS, Keller BB, Srivastava D and Yamagishi H: Springer; Tokyo: pp. 371–376. 2016

9 

Huang CC, Kuo HM, Wu PC, Cheng SH, Chang TT, Chang YC, Kung ML, Wu DC, Chuang JH and Tai MH: Soluble delta-like 1 homolog (DLK1) stimulates angiogenesis through Notch1/Akt/eNOS signaling in endothelial cells. Angiogenesis. 21:299–312. 2018. View Article : Google Scholar : PubMed/NCBI

10 

Shamis Y, Cullen DE, Liu L, Yang G, Ng SF, Xiao L, Bell FT, Ray C, Takikawa S, Moskowitz IP, et al: Maternal and zygotic Zfp57 modulate NOTCH signaling in cardiac development. Proc Natl Acad Sci USA. 112:E2020–2029. 2015. View Article : Google Scholar : PubMed/NCBI

11 

Rodriguez P, Sassi Y, Troncone L, Benard L, Ishikawa K, Gordon RE, Lamas S, Laborda J, Hajjar RJ and Lebeche D: Deletion of delta-like 1 homologue accelerates fibroblast-myofibroblast differentiation and induces myocardial fibrosis. Eur Heart J. 40:967–978. 2019. View Article : Google Scholar : PubMed/NCBI

12 

Zhu H, Wang G and Qian J: Transcription factors as readers and effectors of DNA methylation. Nat Rev Genet. 17:551–565. 2016. View Article : Google Scholar : PubMed/NCBI

13 

Moore-Morris T, van Vliet PP, Andelfinger G and Puceat M: Role of epigenetics in cardiac development and congenital diseases. Physiol Rev. 98:2453–2475. 2018. View Article : Google Scholar : PubMed/NCBI

14 

Serra-Juhé C, Cuscó I, Homs A, Flores R, Torán N and Pérez-Jurado LA: DNA methylation abnormalities in congenital heart disease. Epigenetics. 10:167–177. 2015. View Article : Google Scholar : PubMed/NCBI

15 

Cao J, Wu Q, Huang Y, Wang L, Su Z and Ye H: The role of DNA methylation in syndromic and non-syndromic congenital heart disease. Clin Epigenetics. 13:932021. View Article : Google Scholar : PubMed/NCBI

16 

Sheng W, Qian Y, Wang H, Ma X, Zhang P, Diao L, An Q, Chen L, Ma D and Huang G: DNA methylation status of NKX2-5, GATA4 and HAND1 in patients with tetralogy of fallot. BMC Med Genomics. 6:462013. View Article : Google Scholar : PubMed/NCBI

17 

Fornes O, Castro-Mondragon JA, Khan A, van der Lee R, Zhang X, Richmond PA, Modi BP, Correard S, Gheorghe M, Baranasic D, et al: JASPAR 2020: update of the open-access database of transcription factor binding profiles. Nucleic Acids Res. 48(D1): D87–D92. 2020.PubMed/NCBI

18 

Lacazette E: A laboratory practical illustrating the use of the ChIP-qPCR method in a robust model: Estrogen receptor alpha immunoprecipitation using Mcf-7 culture cells. Biochem Mol Biol Educ. 45:152–160. 2017. View Article : Google Scholar : PubMed/NCBI

19 

Kuhnert F, Kirshner JR and Thurston G: Dll4-Notch signaling as a therapeutic target in tumor angiogenesis. Vasc Cell. 3:202011. View Article : Google Scholar : PubMed/NCBI

20 

MacGrogan D, Münch J and de la Pompa JL: Notch and interacting signalling pathways in cardiac development, disease, and regeneration. Nat Rev Cardiol. 15:685–704. 2018. View Article : Google Scholar : PubMed/NCBI

21 

Luxan G, D'Amato G, MacGrogan D and de la Pompa JL: Endocardial Notch signaling in cardiac development and disease. Circ Res. 118:e1–e18. 2016. View Article : Google Scholar : PubMed/NCBI

22 

Chen H, Zhang W, Sun X, Yoshimoto M, Chen Z, Zhu W, Liu J, Shen Y, Yong W, Li D, et al: Fkbp1a controls ventricular myocardium trabeculation and compaction by regulating endocardial Notch1 activity. Development. 140:1946–1957. 2013. View Article : Google Scholar : PubMed/NCBI

23 

van Weerd JH, Koshiba-Takeuchi K, Kwon C and Takeuchi JK: Epigenetic factors and cardiac development. Cardiovasc Res. 91:203–211. 2011. View Article : Google Scholar : PubMed/NCBI

24 

Wang Z, Zhai W, Richardson JA, Olson EN, Meneses JJ, Firpo MT, Kang C, Skarnes WC and Tjian R: Polybromo protein BAF180 functions in mammalian cardiac chamber maturation. Genes Dev. 18:3106–3116. 2004. View Article : Google Scholar : PubMed/NCBI

25 

Jones PA: Functions of DNA methylation: Islands, start sites, gene bodies and beyond. Nat Rev Genet. 13:484–492. 2012. View Article : Google Scholar : PubMed/NCBI

26 

Zhu Y, Ye M, Xu H, Gu R, Ma X, Chen M, Li X, Sheng W and Huang G: Methylation status of CpG sites in the NOTCH4 promoter region regulates NOTCH4 expression in patients with tetralogy of Fallot. Mol Med Rep. 22:4412–4422. 2020.PubMed/NCBI

27 

Xiaodi L, Ming Y, Hongfei X, Yanjie Z, Ruoyi G, Ma X, Wei S and Guoying H: DNA methylation at CpG island shore and RXRα regulate NR2F2 in heart tissues of tetralogy of Fallot patients. Biochem Biophys Res Commun. 529:1209–1215. 2020. View Article : Google Scholar : PubMed/NCBI

28 

Vohra M, Adhikari P, Souza SC, Nagri SK, Umakanth S, Satyamoorthy K and Rai PS: CpG-SNP site methylation regulates allele-specific expression of MTHFD1 gene in type 2 diabetes. Lab Invest. 100:1090–1101. 2020. View Article : Google Scholar : PubMed/NCBI

29 

Dayeh TA, Olsson AH, Volkov P, Almgren P, Ronn T and Ling C: Identification of CpG-SNPs associated with type 2 diabetes and differential DNA methylation in human pancreatic islets. Diabetologia. 56:1036–1046. 2013. View Article : Google Scholar : PubMed/NCBI

30 

Bogdanović O and Lister R: DNA methylation and the preservation of cell identity. Curr Opin Genet Dev. 46:9–14. 2017. View Article : Google Scholar : PubMed/NCBI

31 

Greenberg MVC and Bourc'his D: The diverse roles of DNA methylation in mammalian development and disease. Nat Rev Mol Cell Biol. 20:590–607. 2019. View Article : Google Scholar : PubMed/NCBI

32 

Bahar Halpern K, Vana T and Walker MD: Paradoxical role of DNA methylation in activation of FoxA2 gene expression during endoderm development. J Biol Chem. 289:23882–23892. 2014. View Article : Google Scholar : PubMed/NCBI

33 

Nabilsi NH, Broaddus RR and Loose DS: DNA methylation inhibits p53-mediated survivin repression. Oncogene. 28:2046–2050. 2009. View Article : Google Scholar : PubMed/NCBI

34 

Jia N, Wang J, Li Q, Tao X, Chang K, Hua K, Yu Y, Wong KK and Feng W: DNA methylation promotes paired box 2 expression via myeloid zinc finger 1 in endometrial cancer. Oncotarget. 7:84785–84797. 2016. View Article : Google Scholar : PubMed/NCBI

35 

Smith J, Sen S, Weeks RJ, Eccles MR and Chatterjee A: Promoter DNA Hypermethylation and Paradoxical Gene Activation. Trends Cancer. 6:392–406. 2020. View Article : Google Scholar : PubMed/NCBI

36 

Ruan H, Liao Y, Ren Z, Mao L, Yao F, Yu P, Ye Y, Zhang Z, Li S, Xu H, et al: Single-cell reconstruction of differentiation trajectory reveals a critical role of ETS1 in human cardiac lineage commitment. BMC Biol. 17:892019. View Article : Google Scholar : PubMed/NCBI

37 

Nie S and Bronner ME: Dual developmental role of transcriptional regulator Ets1 in Xenopus cardiac neural crest vs. heart mesoderm. Cardiovasc Res. 106:67–75. 2015. View Article : Google Scholar : PubMed/NCBI

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Tian G, He L, Gu R, Sun J, Chen W, Qian Y, Ma X, Yan W, Zhao Z, Xu Z, Xu Z, et al: CpG site hypomethylation at ETS1‑binding region regulates DLK1 expression in Chinese patients with Tetralogy of Fallot. Mol Med Rep 25: 93, 2022.
APA
Tian, G., He, L., Gu, R., Sun, J., Chen, W., Qian, Y. ... Huang, G. (2022). CpG site hypomethylation at ETS1‑binding region regulates DLK1 expression in Chinese patients with Tetralogy of Fallot. Molecular Medicine Reports, 25, 93. https://doi.org/10.3892/mmr.2022.12609
MLA
Tian, G., He, L., Gu, R., Sun, J., Chen, W., Qian, Y., Ma, X., Yan, W., Zhao, Z., Xu, Z., Suo, M., Sheng, W., Huang, G."CpG site hypomethylation at ETS1‑binding region regulates DLK1 expression in Chinese patients with Tetralogy of Fallot". Molecular Medicine Reports 25.3 (2022): 93.
Chicago
Tian, G., He, L., Gu, R., Sun, J., Chen, W., Qian, Y., Ma, X., Yan, W., Zhao, Z., Xu, Z., Suo, M., Sheng, W., Huang, G."CpG site hypomethylation at ETS1‑binding region regulates DLK1 expression in Chinese patients with Tetralogy of Fallot". Molecular Medicine Reports 25, no. 3 (2022): 93. https://doi.org/10.3892/mmr.2022.12609
Copy and paste a formatted citation
x
Spandidos Publications style
Tian G, He L, Gu R, Sun J, Chen W, Qian Y, Ma X, Yan W, Zhao Z, Xu Z, Xu Z, et al: CpG site hypomethylation at ETS1‑binding region regulates DLK1 expression in Chinese patients with Tetralogy of Fallot. Mol Med Rep 25: 93, 2022.
APA
Tian, G., He, L., Gu, R., Sun, J., Chen, W., Qian, Y. ... Huang, G. (2022). CpG site hypomethylation at ETS1‑binding region regulates DLK1 expression in Chinese patients with Tetralogy of Fallot. Molecular Medicine Reports, 25, 93. https://doi.org/10.3892/mmr.2022.12609
MLA
Tian, G., He, L., Gu, R., Sun, J., Chen, W., Qian, Y., Ma, X., Yan, W., Zhao, Z., Xu, Z., Suo, M., Sheng, W., Huang, G."CpG site hypomethylation at ETS1‑binding region regulates DLK1 expression in Chinese patients with Tetralogy of Fallot". Molecular Medicine Reports 25.3 (2022): 93.
Chicago
Tian, G., He, L., Gu, R., Sun, J., Chen, W., Qian, Y., Ma, X., Yan, W., Zhao, Z., Xu, Z., Suo, M., Sheng, W., Huang, G."CpG site hypomethylation at ETS1‑binding region regulates DLK1 expression in Chinese patients with Tetralogy of Fallot". Molecular Medicine Reports 25, no. 3 (2022): 93. https://doi.org/10.3892/mmr.2022.12609
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team