Dihydromyricetin ameliorates oxygen‑glucose deprivation and re‑oxygenation‑induced injury in HT22 cells by activating the Wnt/β‑catenin signaling pathway

Tao,Xiaoxiao; Jiang,Yaping; Zheng,Xian; Ji,Xiaoxiao; Peng,Feifei

doi:10.3892/mmr.2022.12619

Dihydromyricetin ameliorates oxygen‑glucose deprivation and re‑oxygenation‑induced injury in HT22 cells by activating the Wnt/β‑catenin signaling pathway

Authors:
- Xiaoxiao Tao
- Yaping Jiang
- Xian Zheng
- Xiaoxiao Ji
- Feifei Peng
View Affiliations

Affiliations: Department of Neurology, Huangyan Hospital of Wenzhou Medical University, Taizhou First People's Hospital, Taizhou, Zhejiang 318020, P.R. China, Department of Clinical Laboratory, Huangyan Hospital of Wenzhou Medical University, Taizhou First People's Hospital, Taizhou, Zhejiang 318020, P.R. China
Published online on: January 26, 2022 https://doi.org/10.3892/mmr.2022.12619
Article Number: 103
Copyright: © Tao et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

Dihydromyricetin (DMY) is a natural flavonoid that possesses a wide range of pharmacological properties. The aim of the present study was to determine whether DMY could protect against nerve cell injury following ischemic stroke through antioxidant and neuroprotective effects. The effects of DMY on the viability, oxidative stress and apoptosis of HT22 cells following oxygen‑glucose deprivation and re‑oxygenation (OGD/R) were examined using MTT, lactate dehydrogenase (LDH), superoxide (SOD), malondialdehyde (MDA), western blot and TUNEL assays. Furthermore, Wnt/β‑catenin signaling proteins in OGD/R‑stimulated HT22 cells were detected in the presence or absence of DMY. In a separate experiment, the effect of DMY on OGD/R‑induced HT22 cell injury was also observed in the presence of the Wnt/β‑catenin inhibitor, XAV939. The results demonstrated that DMY had no impact on the survival of untreated HT22 cells, although DMY treatment significantly increased cell viability and inhibited cytotoxicity, oxidative stress and apoptosis following OGD/R. In addition, DMY upregulated the expression of Wnt/β‑catenin in OGD/R‑stimulated HT22 cells. In conclusion, DMY protected HT22 cells from OGD/R‑induced oxidative stress and apoptosis, and its effects may be mediated by the activation of the Wnt/β‑catenin signaling pathway.

View Figures

View References

1	Katan M and Luft A: Global burden of stroke. Semin Neurol. 38:208–211. 2018. View Article : Google Scholar : PubMed/NCBI
2	Diener HC and Hankey GJ: Primary and secondary prevention of ischemic stroke and cerebral hemorrhage: JACC focus seminar. J Am Coll Cardiol. 75:1804–1818. 2020. View Article : Google Scholar : PubMed/NCBI
3	Suda S, Nito C, Yokobori S, Sakamoto Y, Nakajima M, Sowa K, Obinata H, Sasaki K, Savitz SI and Kimura K: Recent advances in cell-based therapies for ischemic stroke. Int J Mol Sci. 21:67182020. View Article : Google Scholar : PubMed/NCBI
4	Sun MS, Jin H, Sun X, Huang S, Zhang FL, Guo ZN and Yang Y: Free radical damage in ischemia-reperfusion injury: An obstacle in acute ischemic stroke after revascularization therapy. Oxid Med Cell Longev. 2018:38049792018. View Article : Google Scholar : PubMed/NCBI
5	Ulamek-Koziol M, Czuczwar SJ, Januszewski S and Pluta R: Proteomic and genomic changes in tau protein, which are associated with Alzheimer's disease after ischemia-reperfusion brain injury. Int J Mol Sci. 21:8922020. View Article : Google Scholar : PubMed/NCBI
6	Feng Z, Sun Q, Chen W, Bai Y, Hu D and Xie X: The neuroprotective mechanisms of ginkgolides and bilobalide in cerebral ischemic injury: A literature review. Mol Med. 25:572019. View Article : Google Scholar : PubMed/NCBI
7	Bhaskar S, Stanwell P, Cordato D, Attia J and Levi C: Reperfusion therapy in acute ischemic stroke: Dawn of a new era? BMC Neurol. 18:82018. View Article : Google Scholar : PubMed/NCBI
8	Zhang J, Chen Y, Luo H, Sun L, Xu M, Yu J, Zhou Q, Meng G and Yang S: Recent update on the pharmacological effects and mechanisms of dihydromyricetin. Front Pharmacol. 9:12042018. View Article : Google Scholar : PubMed/NCBI
9	Ren ZX, Zhao YF, Cao T and Zhen XC: Dihydromyricetin protects neurons in an MPTP-induced model of Parkinson's disease by suppressing glycogen synthase kinase-3 beta activity. Acta Pharmacol Sin. 37:1315–1324. 2016. View Article : Google Scholar : PubMed/NCBI
10	Wei L, Sun X, Qi X, Zhang Y, Li Y and Xu Y: Dihydromyricetin ameliorates cardiac ischemia/reperfusion injury through Sirt3 activation. Biomed Res Int. 2019:68039432019. View Article : Google Scholar : PubMed/NCBI
11	Chen Y, Lv L, Pi H, Qin W, Chen J, Guo D, Lin J, Chi X, Jiang Z, Yang H and Jiang Y: Dihydromyricetin protects against liver ischemia/reperfusion induced apoptosis via activation of FOXO3a-mediated autophagy. Oncotarget. 7:76508–76522. 2016. View Article : Google Scholar : PubMed/NCBI
12	Liu P, Zou D, Chen K, Zhou Q, Gao Y, Huang Y, Zhu J, Zhang Q and Mi M: Dihydromyricetin improves hypobaric hypoxia-induced memory impairment via modulation of SIRT3 signaling. Mol Neurobiol. 53:7200–7212. 2016. View Article : Google Scholar : PubMed/NCBI
13	Zhang W, Wang S, Yin H, Chen E, Xue D, Zheng Q, Gao X and Pan Z: Dihydromyricetin enhances the osteogenic differentiation of human bone marrow mesenchymal stem cells in vitro partially via the activation of Wnt/β-catenin signaling pathway. Fundam Clin Pharmacol. 30:596–606. 2016. View Article : Google Scholar : PubMed/NCBI
14	Li P, Zhang Y and Liu H: The role of Wnt/β-catenin pathway in the protection process by dexmedetomidine against cerebral ischemia/reperfusion injury in rats. Life Sci. 236:1169212019. View Article : Google Scholar : PubMed/NCBI
15	Du Y, Ma X, Ma L, Li S, Zheng J and Lv J, Cui L and Lv J: Inhibition of microRNA-148b-3p alleviates oxygen-glucose deprivation/reoxygenation-induced apoptosis and oxidative stress in HT22 hippocampal neuron via reinforcing Sestrin2/Nrf2 signalling. Clin Exp Pharmacol Physiol. 47:561–570. 2020. View Article : Google Scholar : PubMed/NCBI
16	Du L, Li X, Zhen L, Chen W, Mu L, Zhang Y and Song A: Everolimus inhibits breast cancer cell growth through PI3K/AKT/mTOR signaling pathway. Mol Med Rep. 17:7163–7169. 2018.PubMed/NCBI
17	Guo Y and Pei X: Tetrandrine-induced autophagy in MDA-MB-231 triple-negative breast cancer cell through the inhibition of PI3K/AKT/mTOR signaling. Evid Based Complement Alternat Med. 2019:75174312019. View Article : Google Scholar : PubMed/NCBI
18	Kangari P, Zarnoosheh Farahany T, Golchin A, Ebadollahzadeh S, Salmaninejad A, Mahboob SA and Nourazarian A: Enzymatic antioxidant and lipid peroxidation evaluation in the newly diagnosed breast cancer patients in Iran. Asian Pac J Cancer Prev. 19:3511–3515. 2018. View Article : Google Scholar : PubMed/NCBI
19	Zhao W, Geng D, Li S, Chen Z and Sun M: LncRNA HOTAIR influences cell growth, migration, invasion, and apoptosis via the miR-20a-5p/HMGA2 axis in breast cancer. Cancer Med. 7:842–855. 2018. View Article : Google Scholar : PubMed/NCBI
20	Huang HL, Wang N, Zhou H and Yu CY: Study on influence of transient ischemic attack on subsequent cerebral infarction. Eur Rev Med Pharmacol Sci. 20:5164–5167. 2016.PubMed/NCBI
21	Cipolla MJ, Liebeskind DS and Chan SL: The importance of comorbidities in ischemic stroke: Impact of hypertension on the cerebral circulation. J Cereb Blood Flow Metab. 38:2129–2149. 2018. View Article : Google Scholar : PubMed/NCBI
22	Paul S and Candelario-Jalil E: Emerging neuroprotective strategies for the treatment of ischemic stroke: An overview of clinical and preclinical studies. Exp Neurol. 335:1135182021. View Article : Google Scholar : PubMed/NCBI
23	Koh SH and Park HH: Neurogenesis in stroke recovery. Transl Stroke Res. 8:3–13. 2017. View Article : Google Scholar : PubMed/NCBI
24	Paci M: Physiotherapy based on the Bobath concept for adults with post-stroke hemiplegia: A review of effectiveness studies. J Rehabil Med. 35:2–7. 2003. View Article : Google Scholar : PubMed/NCBI
25	Gad H, Khan A, Akhtar N, Kamran S, El-Sotouhy A, Dargham SR, Petropoulos IN, Ponirakis G, Shuaib A, Streletz LJ and Malik RA: Corneal nerve and endothelial cell damage in patients with transient ischemic attack and minor ischemic stroke. PLoS One. 14:e02133192019. View Article : Google Scholar : PubMed/NCBI
26	Pertoldi S and Di Benedetto P: Shoulder-hand syndrome after stroke. A complex regional pain syndrome. Eura Medicophys. 41:283–292. 2005.PubMed/NCBI
27	Che F, Du H, Wei J, Zhang W, Cheng Z and Tong Y: MicroRNA-323 suppresses nerve cell toxicity in cerebral infarction via the transforming growth factor-β1/SMAD3 signaling pathway. Int J Mol Med. 43:993–1002. 2019.PubMed/NCBI
28	Guo CH, Cao T, Zheng LT, Waddington JL and Zhen XC: Development and characterization of an inducible Dicer conditional knockout mouse model of Parkinson's disease: Validation of the antiparkinsonian effects of a sigma-1 receptor agonist and dihydromyricetin. Acta Pharmacol Sin. 41:499–507. 2020. View Article : Google Scholar : PubMed/NCBI
29	Zhu H, Luo P, Fu Y, Wang J, Dai J, Shao J, Yang X, Chang L, Weng Q, Yang B and He Q: Dihydromyricetin prevents cardiotoxicity and enhances anticancer activity induced by adriamycin. Oncotarget. 6:3254–3267. 2015. View Article : Google Scholar : PubMed/NCBI
30	Li H, Li Q, Liu Z, Yang K, Chen Z, Cheng Q and Wu L: The versatile effects of dihydromyricetin in health. Evid Based Complement Alternat Med. 2017:10536172017. View Article : Google Scholar : PubMed/NCBI
31	Fan TF, Wu TF, Bu LL, Ma SR, Li YC, Mao L, Sun ZJ and Zhang WF: Dihydromyricetin promotes autophagy and apoptosis through ROS-STAT3 signaling in head and neck squamous cell carcinoma. Oncotarget. 7:59691–59703. 2016. View Article : Google Scholar : PubMed/NCBI
32	Chen N and Wang J: Wnt/β-catenin signaling and obesity. Front Physiol. 9:7922018. View Article : Google Scholar : PubMed/NCBI
33	Zhou L, Chen X, Lu M, Wu Q, Yuan Q, Hu C, Miao J, Zhang Y, Li H, Hou FF, et al: Wnt/β-catenin links oxidative stress to podocyte injury and proteinuria. Kidney Int. 95:830–845. 2019. View Article : Google Scholar : PubMed/NCBI
34	Li P, Wang Y, Liu X, Zhou Z, Wang J, Zhou H, Zheng L and Yang L: Atypical antipsychotics induce human osteoblasts apoptosis via Wnt/β-catenin signaling. BMC Pharmacol Toxicol. 20:102019. View Article : Google Scholar : PubMed/NCBI
35	Jia L, Piña-Crespo J and Li Y: Restoring Wnt/β-catenin signaling is a promising therapeutic strategy for Alzheimer's disease. Mol Brain. 12:1042019. View Article : Google Scholar : PubMed/NCBI