Two PARP13 isoforms are associated with induction of antiviral factors in oral mucosal cells

  • Authors:
    • Hiroki Kato
    • Kouji Ohta
    • Miyuki Sakuma
    • Shohei Fukada
    • Takako Naruse
    • Hideo Shigeishi
    • Hiromi Nishi
    • Masaaki Takechi
  • View Affiliations

  • Published online on: January 31, 2022     https://doi.org/10.3892/mmr.2022.12622
  • Article Number: 106
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Abstract

Innate immune systems in the oral cavity have important roles in the host defense against viral invasion of oral mucosa. Poly(ADP‑ribose) polymerase 13 (PARP13), which has a strong antiviral ability, has been reported to possess two isoforms; a full‑length protein, zinc‑finger antiviral protein long (ZAPL), and a shorter protein (ZAPS). However, the expression and function of these two isoforms in oral mucosa remain unknown. In the present study, the expression levels of ZAPL and ZAPS induced by transfected double‑stranded (ds) RNA, Poly(I:C), and dsDNA, Poly(dA:dT), in immortalized oral keratinocytes and fibroblasts (RT7 and GT1 cell lines, respectively) were investigated. Subsequently, the effects of the knockdown of ZAPL and ZAPS on transfected nucleotide‑induced antiviral factors were examined. The results demonstrated constitutive expression of ZAPL and ZAPS in RT7 and GT1 cells, and their expression in both cell types was notably increased by transfection of Poly(I:C) and Poly(dA:dT) when compared with no transfection. Specific knockdown of ZAPL and ZAPS in RT7 cells decreased IFN‑β and C‑X‑C motif chemokine ligand 10 (CXCL10) expression induced by transfected Poly(I:C) and Poly(dA:dT). On the other hand, knockdown of ZAPL and ZAPS in GT1 cells decreased the expression of CXCL10 induced by the transfected nucleotides, whereas that had no effect on IFN‑β expression induced by Poly(dA:dT). Their knockdown was also associated with transfected nucleotides‑induced IFN regulatory factor 3 phosphorylation in both cell types. Taken together, these results indicate that ZAPL and ZAPS, isoforms of PARP13, in oral mucosal cells participate in host defense against viral infection of oral mucosa.
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March-2022
Volume 25 Issue 3

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Kato H, Ohta K, Sakuma M, Fukada S, Naruse T, Shigeishi H, Nishi H and Takechi M: Two PARP13 isoforms are associated with induction of antiviral factors in oral mucosal cells. Mol Med Rep 25: 106, 2022
APA
Kato, H., Ohta, K., Sakuma, M., Fukada, S., Naruse, T., Shigeishi, H. ... Takechi, M. (2022). Two PARP13 isoforms are associated with induction of antiviral factors in oral mucosal cells. Molecular Medicine Reports, 25, 106. https://doi.org/10.3892/mmr.2022.12622
MLA
Kato, H., Ohta, K., Sakuma, M., Fukada, S., Naruse, T., Shigeishi, H., Nishi, H., Takechi, M."Two PARP13 isoforms are associated with induction of antiviral factors in oral mucosal cells". Molecular Medicine Reports 25.3 (2022): 106.
Chicago
Kato, H., Ohta, K., Sakuma, M., Fukada, S., Naruse, T., Shigeishi, H., Nishi, H., Takechi, M."Two PARP13 isoforms are associated with induction of antiviral factors in oral mucosal cells". Molecular Medicine Reports 25, no. 3 (2022): 106. https://doi.org/10.3892/mmr.2022.12622