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Pulsatilla decoction alleviates colitis by enhancing autophagy and regulating PI3K‑Akt‑mTORC1 signaling pathway

  • Authors:
    • Xuewei Wang
    • Lijun Xu
    • Tao Wang
    • Jian Xu
    • Fugang Fan
    • Yu Zhang
    • Jinpin Wang
    • Qin Cao
  • View Affiliations / Copyright

    Affiliations: Division of Gastroenterology, Putuo Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200062, P.R. China
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 108
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    Published online on: January 31, 2022
       https://doi.org/10.3892/mmr.2022.12624
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Abstract

The aim of the present study was to investigate the therapeutic effect of Pulsatilla decoction (PD) on ulcerative colitis (UC) and to elucidate its potential molecular mechanisms. C57BL/6 mice expressing natural killer (NK)1.1 were used as experimental animals in the present study and a model of oxazolone‑induced colitis was established. Mice were randomly divided into the following five groups: i) PD group; ii) oxazolone‑induced colitis group; iii) IL‑13 intervention group; iv) 5‑aminosalicylic acid positive control group; and v) negative control group (equal volume saline gavage). A total of 10 animals were used in each group. The effects of PD on UC and the association between this regimen and the PI3K‑Akt‑mTORC1 signaling pathway were evaluated by disease activity index (DAI), hematoxylin and eosin staining, reverse transcription‑quantitative PCR (RT‑qPCR), immunofluorescence assay, ELISA and western blotting. The UC models were successfully established by injecting oxazolone gavage solution. Clinical colitis evaluation and histological examination revealed that PD reduced the DAI values in oxazolone‑induced colitis in mice and the degree of infiltration in NK1.1 cells. PD significantly reduced the secretion of IL‑13, as determined using an ELISA. In addition, western blotting and RT‑qPCR analyses demonstrated that Beclin1 and LC3II/I expression levels were downregulated following treatment of the mice with PD. In addition, PD not only partially restored alterations in the expression of tight junction proteins in the colon tissues, but also suppressed the activation of the PI3K‑Akt‑mTORC1 signaling pathway. The data indicated that this regimen could alleviate oxazolone‑induced UC in mice, which could significantly reduce tissue inflammation and autophagy. The mechanism of action was associated with the PI3K‑Akt‑mTORC1 signaling pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Wang X, Xu L, Wang T, Xu J, Fan F, Zhang Y, Wang J and Cao Q: Pulsatilla decoction alleviates colitis by enhancing autophagy and regulating PI3K‑Akt‑mTORC1 signaling pathway. Mol Med Rep 25: 108, 2022.
APA
Wang, X., Xu, L., Wang, T., Xu, J., Fan, F., Zhang, Y. ... Cao, Q. (2022). Pulsatilla decoction alleviates colitis by enhancing autophagy and regulating PI3K‑Akt‑mTORC1 signaling pathway. Molecular Medicine Reports, 25, 108. https://doi.org/10.3892/mmr.2022.12624
MLA
Wang, X., Xu, L., Wang, T., Xu, J., Fan, F., Zhang, Y., Wang, J., Cao, Q."Pulsatilla decoction alleviates colitis by enhancing autophagy and regulating PI3K‑Akt‑mTORC1 signaling pathway". Molecular Medicine Reports 25.3 (2022): 108.
Chicago
Wang, X., Xu, L., Wang, T., Xu, J., Fan, F., Zhang, Y., Wang, J., Cao, Q."Pulsatilla decoction alleviates colitis by enhancing autophagy and regulating PI3K‑Akt‑mTORC1 signaling pathway". Molecular Medicine Reports 25, no. 3 (2022): 108. https://doi.org/10.3892/mmr.2022.12624
Copy and paste a formatted citation
x
Spandidos Publications style
Wang X, Xu L, Wang T, Xu J, Fan F, Zhang Y, Wang J and Cao Q: Pulsatilla decoction alleviates colitis by enhancing autophagy and regulating PI3K‑Akt‑mTORC1 signaling pathway. Mol Med Rep 25: 108, 2022.
APA
Wang, X., Xu, L., Wang, T., Xu, J., Fan, F., Zhang, Y. ... Cao, Q. (2022). Pulsatilla decoction alleviates colitis by enhancing autophagy and regulating PI3K‑Akt‑mTORC1 signaling pathway. Molecular Medicine Reports, 25, 108. https://doi.org/10.3892/mmr.2022.12624
MLA
Wang, X., Xu, L., Wang, T., Xu, J., Fan, F., Zhang, Y., Wang, J., Cao, Q."Pulsatilla decoction alleviates colitis by enhancing autophagy and regulating PI3K‑Akt‑mTORC1 signaling pathway". Molecular Medicine Reports 25.3 (2022): 108.
Chicago
Wang, X., Xu, L., Wang, T., Xu, J., Fan, F., Zhang, Y., Wang, J., Cao, Q."Pulsatilla decoction alleviates colitis by enhancing autophagy and regulating PI3K‑Akt‑mTORC1 signaling pathway". Molecular Medicine Reports 25, no. 3 (2022): 108. https://doi.org/10.3892/mmr.2022.12624
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