Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Molecular Medicine Reports
Join Editorial Board Propose a Special Issue
Print ISSN: 1791-2997 Online ISSN: 1791-3004
Journal Cover
April-2022 Volume 25 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
April-2022 Volume 25 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML

  • Supplementary Files
    • Supplementary_Data.pdf
Article Open Access

Molecular mechanism of atractylon in the invasion and migration of hepatic cancer cells based on high‑throughput sequencing

Retraction in: /10.3892/mmr.2024.13373
  • Authors:
    • Yang Cheng
    • Jian Ping
    • Jianjie Chen
    • Yifei Fu
    • Hui Zhao
    • Jiahua Xue
  • View Affiliations / Copyright

    Affiliations: Institute of Liver Disease, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 201203, P.R. China, Institute of Liver Disease, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 201203, P.R. China, Department of Liver Disease, Hospital for Infectious Diseases of Pudong District, Shanghai 201299, P.R. China
    Copyright: © Cheng et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 112
    |
    Published online on: February 2, 2022
       https://doi.org/10.3892/mmr.2022.12628
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

The aim of the present study was to investigate the molecular mechanisms of atractylon in the inhibition of invasion and migration of hepatic cancer cells. High‑throughput sequencing was used to compare the expression of long non‑coding (lnc)RNAs between hepatic carcinoma and healthy controls. A competing endogenous RNA network was constructed. The top significantly differentially expressed lncRNAs were screened and verified by reverse transcription‑quantitative PCR in vitro and in vivo. Small interfering (si)RNA against thymopoietin‑antisense 1 (TMPO‑AS1) or coiled‑coil domain‑containing 183‑antisense 1 (CCDC183‑AS1) overexpression (oe) vectors were transfected into cells following atractylon treatment. Wound healing and Matrigel assays were used to determine the effects of migration and invasion, respectively. Western blot analysis was used to detect the expression levels of invasion‑ and migration‑related proteins, including N‑cadherin, E‑cadherin and MMP‑2. Flow cytometry analysis was used to detect apoptosis. Based on transcriptome sequencing and analysis, the top seven upregulated [(FAM201A, RP11‑640M9.2, AL589743.1, TMEM51‑AS1, clathrin heavy chain‑like 1 (CLTCL1), TMPO‑AS1 and LINC00652] and top six downregulated lncRNAs (RP11‑465B22.5, CCDC183‑AS1, TCONS_00072529, RP11‑401F2.3, RP11‑290F20.1 and TCONS_00070568) were identified. Only TMPO‑AS1 and CCDC183‑AS1 were differently regulated by atractylon in vivo. The proliferative ability of HepG2 liver cancer cells decreased, whereas the apoptotic rate improved after atractylon treatment. Notably, the invasive and migratory ability of HepG2 cells significantly declined. In addition, siTMPO‑AS1 and oeCCDC183‑AS1 reduced the effect of atractylon in vitro. Atractylon was demonstrated to regulate the expression of TMPO‑AS1 and CCDC183‑AS1 and inhibited the invasion and migration of liver cancer cells. Thus, TMPO‑AS1 and CCDC183‑AS1 may be potential targets for diagnosis and treatment of hepatic carcinoma.
View Figures

Figure 1

Figure 2

Figure 3

Figure 4

Figure 5

Figure 6

Figure 7

View References

1 

An L, Zeng HM, Zheng RS, Zhang SW, Sun KX, Zou XN, Chen R, Wang SM, Gu XY, Wei WW and He J: Liver cancer epidemiology in china, 2015. Zhonghua Zhong Liu Za Zhi. 41:721–727. 2019.(In Chinese). PubMed/NCBI

2 

Ding C, Fu X, Zhou Y, Liu X, Wu J, Huang C, Deng M, Li Y, Li L and Yang S: Disease burden of liver cancer in China from 1997 to 2016: An observational study based on the global burden of diseases. BMJ Open. 9:e0256132019. View Article : Google Scholar : PubMed/NCBI

3 

Fukui N, Golabi P, Otgonsuren M, de Avila L, Bush H and Younossi ZM: Hospice care in medicare patients with primary liver cancer: The impact on resource utilisation and mortality. Aliment Pharmacol Ther. 47:680–688. 2018. View Article : Google Scholar : PubMed/NCBI

4 

Fisher RA, Maluf DG, Wolfe L, Williams B, Cotterell A, Stravitz RT, Heuman D and Posner M: Is hepatic transplantation justified for primary liver cancer? J Surg Oncol. 95:674–679. 2007. View Article : Google Scholar : PubMed/NCBI

5 

Pan YX, Chen JC, Fang AP, Wang XH, Chen JB, Wang JC, He W, Fu YZ, Xu L, Chen MS, et al: A nomogram predicting the recurrence of hepatocellular carcinoma in patients after laparoscopic hepatectomy. Cancer Commun (Lond). 39:552019. View Article : Google Scholar : PubMed/NCBI

6 

Li J, Wang X, Ma M, et al: Liver transplantation for non-hepatocellular carcinoma malignant liver tumors: A single center case study. Pract J Organ Transplant. 7:44–47. 2019.

7 

Kim HK, Yun YK and Ahn YJ: Toxicity of atractylon and atractylenolide III identified in atractylodes ovata rhizome to dermatophagoides farinae and dermatophagoides pteronyssinus. J Agric Food Chem. 55:6027–6031. 2007. View Article : Google Scholar : PubMed/NCBI

8 

Makówka A, Olejniczak-Fortak M and Nowicki M: A comparison of the antihypertensive and anti-inflammatory effects of aliskiren and ramipril add-on therapy in peritoneal dialysis patients-a pilot open label study. Kidney Blood Press Res. 36:18–25. 2012. View Article : Google Scholar : PubMed/NCBI

9 

Chen LG, Jan YS, Tsai PW, Norimoto H, Michihara S, Murayama C and Wang CC: Anti-inflammatory and antinociceptive constituents of atractylodes japonica koidzumi. J Agric Food Chem. 64:2254–2262. 2016. View Article : Google Scholar : PubMed/NCBI

10 

Hwang JM, Tseng TH, Hsieh YS, Chou FP, Wang CJ and Chu CY: Inhibitory effect of atractylon on tert-butyl hydroperoxide induced DNA damage and hepatic toxicity in rat hepatocytes. Arch Toxicol. 70:640–644. 1996. View Article : Google Scholar : PubMed/NCBI

11 

Tin MM, Cho CH, Chan K, James AE and Ko JK: Astragalus saponins induce growth inhibition and apoptosis in human colon cancer cells and tumor xenograft. Carcinogenesis. 28:1347–1355. 2007. View Article : Google Scholar : PubMed/NCBI

12 

Tao Y, Zhan S, Wang Y, Zhou G, Liang H, Chen X and Shen H: Baicalin, the major component of traditional Chinese medicine Scutellaria baicalensis induces colon cancer cell apoptosis through inhibition of oncomiRNAs. Sci Rep. 8:144772018. View Article : Google Scholar : PubMed/NCBI

13 

Cheng X, Su X, Chen X, Zhao H, Bo C, Xu J, Bai H and Ning K: Biological ingredient analysis of traditional Chinese medicine preparation based on high-throughput sequencing: The story for Liuwei Dihuang Wan. Sci Rep. 4:51472014. View Article : Google Scholar : PubMed/NCBI

14 

Nichols AC, Black M, Yoo J, Pinto N, Fernandes A, Haibe-Kains B, Boutros PC and Barrett JW: Exploiting high-throughput cell line drug screening studies to identify candidate therapeutic agents in head and neck cancer. BMC Pharmacol Toxicol. 15:662014. View Article : Google Scholar : PubMed/NCBI

15 

Cheng Y, Chen T, Yang X, Xue J and Chen J: Atractylon induces apoptosis and suppresses metastasis in hepatic cancer cells and inhibits growth in vivo. Cancer Manag Res. 11:5883–5894. 2019. View Article : Google Scholar : PubMed/NCBI

16 

Mane VP, Heuer MA, Hillyer P, Navarro MB and Rabin RL: Systematic method for determining an ideal housekeeping gene for real-time PCR analysis. J Biomol Tech. 19:342–347. 2008.PubMed/NCBI

17 

Pertea M, Pertea GM, Antonescu CM, Chang TC, Mendell JT and Salzberg SL: StringTie enables improved reconstruction of a transcriptome from RNA-seq reads. Nat Biotechnol. 33:290–295. 2015. View Article : Google Scholar : PubMed/NCBI

18 

Cheng Y, Geng L, Wang K, Sun J, Xu W, Gong S and Zhu Y: Long noncoding RNA expression signatures of colon cancer based on the ceRNA network and their prognostic value. Dis Markers. 2019:76367572019. View Article : Google Scholar : PubMed/NCBI

19 

Zhao C and He C: Preparative isolation and purification of atractylon and atractylenolide III from the Chinese medicinal plant Atractylodes macrocephala by high-speed counter-current chromatography. J Sep Sci. 29:1630–1636. 2006. View Article : Google Scholar : PubMed/NCBI

20 

Kiso Y, Tohkin M and Hikino H: Antihepatotoxic principles of atractylodes rhizomes. J Nat Prod. 46:651–654. 1983. View Article : Google Scholar : PubMed/NCBI

21 

Cui XB, Shan CX, Wen HM, Wei L and Hao W: UFLC/Q-TOF-MS based analysis on material base of atractylodis macrocephalae rhizoma stir-fried with wheat bran. Zhongguo Zhong Yao Za Zhi. 38:1929–1933. 2013.(In Chinese). PubMed/NCBI

22 

Cheng Y, Mai JY, Hou TL, Ping J and Chen JJ: Antiviral activities of atractylon from atractylodis rhizoma. Mol Med Rep. 14:3704–3710. 2016. View Article : Google Scholar : PubMed/NCBI

23 

Shou D, Dai S, Zhang J, Li H and Yu Z: Simultaneous determination of atractylenolide III, atractylenolide I and atractylon in artactylodis macrocephala using microbore liquid chromatography. Se Pu. 26:637–639. 2008.(In Chinese). PubMed/NCBI

24 

Satoh K, Nagai F, Ushiyama K and Kano I: Specific inhibition of Na+,K(+)-ATPase activity by atractylon, a major component of byaku-jutsu, by interaction with enzyme in the E2 state. Biochem Pharmacol. 51:339–343. 1996. View Article : Google Scholar : PubMed/NCBI

25 

Tang DN, Wei JM, Zhu ZM, Wen HX and Guo LY: Experimental study the on relationship between inhibiting the ubiquitin-proteasome pathway and drug sensitivity in human liver carcinoma cell. Chin J Hepatobiliary Surg. 9:634–636. 2006.(In Chinese).

26 

Li DS, Ainiwaer JL, Sheyhiding I, Zhang Z and Zhang LW: Identification of key long non-coding RNAs as competing endogenous RNAs for miRNA-mRNA in lung adenocarcinoma. Eur Rev Med Pharmacol Sci. 20:2285–2295. 2016.PubMed/NCBI

27 

Mu X, Wu H, Liu J, Hu X, Wu H, Chen L, Liu W, Luo S and Zhao Y: Long noncoding RNA TMPO-AS1 promotes lung adenocarcinoma progression and is negatively regulated by miR-383-5p. Biomed Pharmacother. 125:1099892020. View Article : Google Scholar : PubMed/NCBI

28 

Huang W, Su X, Yan W, Kong Z, Wang D, Huang Y, Zhai Q, Zhang X, Wu H, Li Y, et al: Overexpression of AR-regulated lncRNA TMPO-AS1 correlates with tumor progression and poor prognosis in prostate cancer. Prostate. 78:1248–1261. 2018. View Article : Google Scholar : PubMed/NCBI

29 

Qin Z, Zheng X and Fang Y: Long noncoding RNA TMPO-AS1 promotes progression of non-small cell lung cancer through regulating its natural antisense transcript TMPO. Biochem Biophys Res Commun. 516:486–493. 2019. View Article : Google Scholar : PubMed/NCBI

30 

Simsir A, Fetsch P, Mehta D, Zakowski M and Abati A: E-cadherin, N-cadherin, and calretinin in pleural effusions: The good, the bad, the worthless. Diagn Cytopathol. 20:125–130. 1999. View Article : Google Scholar : PubMed/NCBI

31 

Andersen H, Mejlvang J, Mahmood S, Gromova I, Gromov P, Lukanidin E, Kriajevska M, Mellon JK and Tulchinsky E: Immediate and delayed effects of E-cadherin inhibition on gene regulation and cell motility in human epidermoid carcinoma cells. Mol Cell Biol. 25:9138–9150. 2005. View Article : Google Scholar : PubMed/NCBI

32 

Lin CJ, Lin CY, Wu JC, Huang SH, Wang SM and Chen GH: 357 Macrophage activation promotes invasive properties of hepatocellular carcinoma cells through destablization of the E-cadherin/B-catenin complex. J Hepatol. 44 (Suppl 2):S1362006. View Article : Google Scholar

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Cheng Y, Ping J, Chen J, Fu Y, Zhao H and Xue J: Molecular mechanism of atractylon in the invasion and migration of hepatic cancer cells based on high‑throughput sequencing Retraction in /10.3892/mmr.2024.13373. Mol Med Rep 25: 112, 2022.
APA
Cheng, Y., Ping, J., Chen, J., Fu, Y., Zhao, H., & Xue, J. (2022). Molecular mechanism of atractylon in the invasion and migration of hepatic cancer cells based on high‑throughput sequencing Retraction in /10.3892/mmr.2024.13373. Molecular Medicine Reports, 25, 112. https://doi.org/10.3892/mmr.2022.12628
MLA
Cheng, Y., Ping, J., Chen, J., Fu, Y., Zhao, H., Xue, J."Molecular mechanism of atractylon in the invasion and migration of hepatic cancer cells based on high‑throughput sequencing Retraction in /10.3892/mmr.2024.13373". Molecular Medicine Reports 25.4 (2022): 112.
Chicago
Cheng, Y., Ping, J., Chen, J., Fu, Y., Zhao, H., Xue, J."Molecular mechanism of atractylon in the invasion and migration of hepatic cancer cells based on high‑throughput sequencing Retraction in /10.3892/mmr.2024.13373". Molecular Medicine Reports 25, no. 4 (2022): 112. https://doi.org/10.3892/mmr.2022.12628
Copy and paste a formatted citation
x
Spandidos Publications style
Cheng Y, Ping J, Chen J, Fu Y, Zhao H and Xue J: Molecular mechanism of atractylon in the invasion and migration of hepatic cancer cells based on high‑throughput sequencing Retraction in /10.3892/mmr.2024.13373. Mol Med Rep 25: 112, 2022.
APA
Cheng, Y., Ping, J., Chen, J., Fu, Y., Zhao, H., & Xue, J. (2022). Molecular mechanism of atractylon in the invasion and migration of hepatic cancer cells based on high‑throughput sequencing Retraction in /10.3892/mmr.2024.13373. Molecular Medicine Reports, 25, 112. https://doi.org/10.3892/mmr.2022.12628
MLA
Cheng, Y., Ping, J., Chen, J., Fu, Y., Zhao, H., Xue, J."Molecular mechanism of atractylon in the invasion and migration of hepatic cancer cells based on high‑throughput sequencing Retraction in /10.3892/mmr.2024.13373". Molecular Medicine Reports 25.4 (2022): 112.
Chicago
Cheng, Y., Ping, J., Chen, J., Fu, Y., Zhao, H., Xue, J."Molecular mechanism of atractylon in the invasion and migration of hepatic cancer cells based on high‑throughput sequencing Retraction in /10.3892/mmr.2024.13373". Molecular Medicine Reports 25, no. 4 (2022): 112. https://doi.org/10.3892/mmr.2022.12628
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team