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Propofol postconditioning alleviates diabetic myocardial ischemia‑reperfusion injury via the miR‑200c‑3p/AdipoR2/STAT3 signaling pathway

  • Authors:
    • Lijun Huang
    • Li Ding
    • Shenghui Yu
    • Xin Huang
    • Qiusheng Ren
  • View Affiliations / Copyright

    Affiliations: Department of Anesthesiology, The Affiliated People's Hospital of Ningbo University, Ningbo, Zhejiang 315040, P.R. China
    Copyright: © Huang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 137
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    Published online on: February 22, 2022
       https://doi.org/10.3892/mmr.2022.12653
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Abstract

Myocardial ischemia/reperfusion (MI/RI) syndrome is one of the leading causes of mortality and disability. Propofol postconditioning is known to improve myocardial ischemia/reperfusion injury (MI/RI). The present study aimed to explore the mechanism of propofol postconditioning in diabetic MI/RI. Diabetic MI/RI rat models were established and the rats were treated via propofol postconditioning. Staining with 2,3,5‑triphenyl‑2H‑tetrazolium chloride, H&E staining, TUNEL staining and ELISA were applied to detect infarct size, pathological changes, apoptosis and oxidative stress‑related factor and apoptotic factor levels, respectively. Subsequently, the effect of propofol on H9C2 cells was also assessed using the Cell Counting Kit‑8 assay. High‑glucose hypoxia/reperfusion (H/R) models of H9C2 cardiomyocytes were established. miR‑200c‑3p overexpression or AdipoR2 silencing combined with propofol postconditioning was performed in H/R‑induced H9C2 cells and STAT3 protein expression levels were determined. Propofol postconditioning significantly reduced myocardial infarct size, oxidative stress and apoptosis in diabetic MI/RI models. Furthermore, propofol postconditioning significantly reduced the oxidative stress and apoptosis of H9C2 cells in high‑glucose H/R models. Propofol postconditioning also significantly downregulated miR‑200c‑3p expression levels and promoted AdipoR2 expression levels. miR‑200c‑3p overexpression or AdipoR2 downregulation significantly reversed the effects of propofol postconditioning on its antioxidation and anti‑apoptotic effects in H9C2 cells and on decreasing STAT3 phosphorylation levels. Together, the results of the present study demonstrated that propofol postconditioning inhibited miR‑200c‑3p, upregulated AdipoR2 and activated the STAT3 signaling pathway, thus alleviating diabetic MI/RI and therefore highlighting its potential as a treatment of diabetic MI/RI.
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Copy and paste a formatted citation
Spandidos Publications style
Huang L, Ding L, Yu S, Huang X and Ren Q: Propofol postconditioning alleviates diabetic myocardial ischemia‑reperfusion injury <em>via</em> the miR‑200c‑3p/AdipoR2/STAT3 signaling pathway. Mol Med Rep 25: 137, 2022.
APA
Huang, L., Ding, L., Yu, S., Huang, X., & Ren, Q. (2022). Propofol postconditioning alleviates diabetic myocardial ischemia‑reperfusion injury <em>via</em> the miR‑200c‑3p/AdipoR2/STAT3 signaling pathway. Molecular Medicine Reports, 25, 137. https://doi.org/10.3892/mmr.2022.12653
MLA
Huang, L., Ding, L., Yu, S., Huang, X., Ren, Q."Propofol postconditioning alleviates diabetic myocardial ischemia‑reperfusion injury <em>via</em> the miR‑200c‑3p/AdipoR2/STAT3 signaling pathway". Molecular Medicine Reports 25.4 (2022): 137.
Chicago
Huang, L., Ding, L., Yu, S., Huang, X., Ren, Q."Propofol postconditioning alleviates diabetic myocardial ischemia‑reperfusion injury <em>via</em> the miR‑200c‑3p/AdipoR2/STAT3 signaling pathway". Molecular Medicine Reports 25, no. 4 (2022): 137. https://doi.org/10.3892/mmr.2022.12653
Copy and paste a formatted citation
x
Spandidos Publications style
Huang L, Ding L, Yu S, Huang X and Ren Q: Propofol postconditioning alleviates diabetic myocardial ischemia‑reperfusion injury <em>via</em> the miR‑200c‑3p/AdipoR2/STAT3 signaling pathway. Mol Med Rep 25: 137, 2022.
APA
Huang, L., Ding, L., Yu, S., Huang, X., & Ren, Q. (2022). Propofol postconditioning alleviates diabetic myocardial ischemia‑reperfusion injury <em>via</em> the miR‑200c‑3p/AdipoR2/STAT3 signaling pathway. Molecular Medicine Reports, 25, 137. https://doi.org/10.3892/mmr.2022.12653
MLA
Huang, L., Ding, L., Yu, S., Huang, X., Ren, Q."Propofol postconditioning alleviates diabetic myocardial ischemia‑reperfusion injury <em>via</em> the miR‑200c‑3p/AdipoR2/STAT3 signaling pathway". Molecular Medicine Reports 25.4 (2022): 137.
Chicago
Huang, L., Ding, L., Yu, S., Huang, X., Ren, Q."Propofol postconditioning alleviates diabetic myocardial ischemia‑reperfusion injury <em>via</em> the miR‑200c‑3p/AdipoR2/STAT3 signaling pathway". Molecular Medicine Reports 25, no. 4 (2022): 137. https://doi.org/10.3892/mmr.2022.12653
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