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Role and mechanism of the lncRNA SNHG1/miR‑450b‑5p/IGF1 axis in the regulation of myocardial ischemia reperfusion injury

  • Authors:
    • Junfeng Zhan
    • Qiulin Yin
    • Peng Zhao
    • Lang Hong
  • View Affiliations / Copyright

    Affiliations: Department of Cardiology, Jiangxi Provincial People's Hospital Affiliated to Nanchang University, Nanchang, Jiangxi 330006, P.R. China, Department of Cardiology, People's Hospital of Zixi County, Fuzhou, Jiangxi 335300, P.R. China
    Copyright: © Zhan et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 176
    |
    Published online on: March 21, 2022
       https://doi.org/10.3892/mmr.2022.12692
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Abstract

The increasing rates of morbidity and mortality caused by ischemic heart disease pose a serious threat to human health. Long non‑coding (lnc)RNA small nucleolar RNA host gene 1 (SNHG1) has a protective effect on the myocardium. In the present study, the role of lncRNA SNHG1 in myocardial ischemia reperfusion injury (MIRI) and the underlying mechanisms were investigated. After hypoxia/reoxygenation (H/R) induction, the expression levels of lncRNA SNHG1 were detected using reverse transcription‑quantitative PCR. After lncRNA SNHG1 overexpression via cell transfection, cell viability was detected using an MTT assay, apoptotic rates were detected using TUNEL staining, apoptosis‑related protein expression levels were detected using western blotting and respective kits were used to measure the oxidative stress levels. The Encyclopedia of RNA Interactomes database predicted the presence of binding sites between lncRNA SNHG1 and microRNA (miR)‑450b‑5p, and between miR‑450b‑5p and insulin‑like growth factor 1 (IGF1). These interactions were then verified using luciferase reporter assays. Subsequently, the regulatory mechanism underlying the lncRNA SNHG1/miR‑450b‑5p/IGF1 axis in MIRI was investigated by overexpressing miR‑450b‑5p and knocking down IGF1 expression in H/R‑induced cells. Finally, the expression of PI3K/Akt signaling pathway‑related proteins was detected using western blotting. lncRNA SNHG1 expression was significantly downregulated in H/R‑induced AC16 cells. lncRNA SNHG1 overexpression significantly inhibited apoptosis and decreased oxidative stress levels in H/R‑induced AC16 cells, which was mediated via regulation of the miR‑450b‑5p/IGF1 axis and activation of the PI3K/Akt signaling pathway. Therefore, the present study suggested that activation of the PI3K/Akt signaling pathway via the lncRNA SNHG1/miR‑450b‑5p/IGF1 axis inhibited the apoptosis and oxidative stress levels of H/R‑induced AC16 cells.
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1 

Severino P, D'Amato A, Pucci M, Infusino F, Adamo F, Birtolo LI, Netti L, Montefusco G, Chimenti C, Lavalle C, et al: Ischemic heart disease pathophysiology paradigms overview: From plaque activation to microvascular dysfunction. Int J Mol Sci. 21:81182020. View Article : Google Scholar : PubMed/NCBI

2 

Kaski JC, Crea F, Gersh BJ and Camici PG: Reappraisal of ischemic heart disease. Circulation. 138:1463–1480. 2018. View Article : Google Scholar : PubMed/NCBI

3 

Frank A, Bonney M, Bonney S, Weitzel L, Koeppen M and Eckle T: Myocardial ischemia reperfusion injury: From basic science to clinical bedside. Semin Cardiothorac Vasc Anesth. 16:123–132. 2012. View Article : Google Scholar : PubMed/NCBI

4 

Wang J, Su Z, Lu S, Fu W, Liu Z, Jiang X and Tai S: LncRNA HOXA-AS2 and its molecular mechanisms in human cancer. Clin Chim Acta. 485:229–233. 2018. View Article : Google Scholar : PubMed/NCBI

5 

Thin KZ, Tu JC and Raveendran S: Long non-coding SNHG1 in cancer. Clin Chim Acta. 494:38–47. 2019. View Article : Google Scholar : PubMed/NCBI

6 

Yan SM, Li H, Shu Q, Wu WJ, Luo XM and Lu L: LncRNA SNHG1 exerts a protective role in cardiomyocytes hypertrophy via targeting miR-15a-5p/HMGA1 axis. Cell Biol Int. 44:1009–1019. 2020. View Article : Google Scholar : PubMed/NCBI

7 

Liu Y, Yang Y, Li L, Liu Y, Geng P, Li G and Song H: LncRNA SNHG1 enhances cell proliferation, migration, and invasion in cervical cancer. Biochem Cell Biol. 96:38–43. 2018. View Article : Google Scholar : PubMed/NCBI

8 

Zhang Z and Wang H: lncRNA SNHG1 suppresses gastric cancer cell proliferation and promotes apoptosis via Notch1 pathway. J BUON. 25:302–307. 2020.PubMed/NCBI

9 

Chen S, Wang J and Zhou Y: Long non-coding RNA SNHG1 protects human AC16 cardiomyocytes from doxorubicin toxicity by regulating miR-195/Bcl-2 axis. Biosci Rep. Jul 25–2019.(Epub ahead of print). doi: 10.1042/BSR20191050. View Article : Google Scholar

10 

Liang S, Ren K, Li B, Li F, Liang Z, Hu J, Xu B and Zhang A: LncRNA SNHG1 alleviates hypoxia-reoxygenation-induced vascular endothelial cell injury as a competing endogenous RNA through the HIF-1α/VEGF signal pathway. Mol Cell Biochem. 465:1–11. 2020. View Article : Google Scholar : PubMed/NCBI

11 

Huang Y: The novel regulatory role of lncRNA-miRNA-mRNA axis in cardiovascular diseases. J Cell Mol Med. 22:5768–5775. 2018. View Article : Google Scholar : PubMed/NCBI

12 

Huang Z, Mou T, Luo Y, Pu X, Pu J, Wan L, Gong J, Yang H, Liu Y, Li Z, et al: Inhibition of miR-450b-5p ameliorates hepatic ischemia/reperfusion injury via targeting CRYAB. Cell Death Dis. 11:4552020. View Article : Google Scholar : PubMed/NCBI

13 

Liao Y, Li H, Pi Y, Li Z and Jin S: Cardioprotective effect of IGF-1 against myocardial ischemia/reperfusion injury through activation of PI3K/Akt pathway in rats in vivo. J Int Med Res. 47:3886–3897. 2019. View Article : Google Scholar : PubMed/NCBI

14 

Sheng M, Huang Z, Pan L, Yu M, Yi C, Teng L, He L, Gu C, Xu C and Li J: SOCS2 exacerbates myocardial injury induced by ischemia/reperfusion in diabetic mice and H9c2 cells through inhibiting the JAK-STAT-IGF-1 pathway. Life Sci. 188:101–109. 2017. View Article : Google Scholar : PubMed/NCBI

15 

Li W, Dong X, He C, Tan G, Li Z, Zhai B, Feng J, Jiang X, Liu C, Jiang H and Sun X: LncRNA SNHG1 contributes to sorafenib resistance by activating the Akt pathway and is positively regulated by miR-21 in hepatocellular carcinoma cells. J Exp Clin Cancer Res. 38:1832019. View Article : Google Scholar : PubMed/NCBI

16 

Chen J, Zhang W, Wu YQ, Chen H and Zhao JF: LncRNA SNHG1 inhibits neuronal apoptosis in cerebral infarction rats through PI3K/Akt signaling pathway. Eur Rev Med Pharmacol Sci. 23:5366–5373. 2019.PubMed/NCBI

17 

Tang M, Pan H, Zheng Z, Guo Y, Peng J, Yang J, Luo Y, He J, Yan S, Wang P, et al: Prostaglandin E1 protects cardiomyocytes against hypoxia-reperfusion induced injury via the miR-21-5p/FASLG axis. Biosci Rep. 39:BSR201905972019. View Article : Google Scholar : PubMed/NCBI

18 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

19 

Eltzschig HK and Eckle T: Ischemia and reperfusion-from mechanism to translation. Nat Med. 17:1391–1401. 2011. View Article : Google Scholar : PubMed/NCBI

20 

Xiao X, Tan Z, Jia M, Zhou X, Wu K, Ding Y and Li W: Long Noncoding RNA SNHG1 knockdown ameliorates apoptosis, oxidative stress and inflammation in models of Parkinson's disease by inhibiting the miR-125b-5p/MAPK1 Axis. Neuropsychiatr Dis Treat. 17:1153–1163. 2021. View Article : Google Scholar : PubMed/NCBI

21 

Zhang N, Meng X, Mei L, Hu J, Zhao C and Chen W: The long non-coding RNA SNHG1 attenuates cell apoptosis by regulating miR-195 and BCL2-Like protein 2 in human cardiomyocytes. Cell Physiol Biochem. 50:1029–1040. 2018. View Article : Google Scholar : PubMed/NCBI

22 

Delic D, Dkhil M, Al-Quraishy S and Wunderlich F: Hepatic miRNA expression reprogrammed by Plasmodium chabaudi malaria. Parasitol Res. 108:1111–1121. 2011. View Article : Google Scholar : PubMed/NCBI

23 

Ohuchi K, Watanabe M, Hirasawa N, Tsurufuji S, Ozeki T and Fujiki H: Inhibition by gossypol of tumor promoter-induced arachidonic acid metabolism in rat peritoneal macrophages. Biochim Biophys Acta. 971:85–91. 1988. View Article : Google Scholar : PubMed/NCBI

24 

Wang L, Niu X, Hu J, Xing H, Sun M, Wang J, Jian Q and Yang H: After myocardial ischemia-reperfusion, miR-29a, and Let7 could affect apoptosis through regulating IGF-1. Biomed Res Int. 2015:2454122015. View Article : Google Scholar : PubMed/NCBI

25 

Song CL, Liu B, Diao HY, Shi YF, Zhang JC, Li YX, Liu N, Yu YP, Wang G, Wang JP and Li Q: Down-regulation of microRNA-320 suppresses cardiomyocyte apoptosis and protects against myocardial ischemia and reperfusion injury by targeting IGF-1. Oncotarget. 7:39740–39757. 2016. View Article : Google Scholar : PubMed/NCBI

26 

Tejada T, Tan L, Torres RA, Calvert JW, Lambert JP, Zaidi M, Husain M, Berce MD, Naib H, Pejler G, et al: IGF-1 degradation by mouse mast cell protease 4 promotes cell death and adverse cardiac remodeling days after a myocardial infarction. Proc Natl Acad Sci USA. 113:6949–6954. 2016. View Article : Google Scholar : PubMed/NCBI

27 

Arslan F, Lai RC, Smeets MB, Akeroyd L, Choo A, Aguor EN, Timmers L, van Rijen HV, Doevendans PA, Pasterkamp G, et al: Mesenchymal stem cell-derived exosomes increase ATP levels, decrease oxidative stress and activate PI3K/Akt pathway to enhance myocardial viability and prevent adverse remodeling after myocardial ischemia/reperfusion injury. Stem Cell Res. 10:301–312. 2013. View Article : Google Scholar : PubMed/NCBI

28 

Chen E, Chen C, Niu Z, Gan L, Wang Q, Li M, Cai X, Gao R, Katakam S, Chen H, et al: Poly(I:C) preconditioning protects the heart against myocardial ischemia/reperfusion injury through TLR3/PI3K/Akt-dependent pathway. Signal Transduct Target Ther. 5:2162020. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Zhan J, Yin Q, Zhao P and Hong L: Role and mechanism of the lncRNA SNHG1/miR‑450b‑5p/IGF1 axis in the regulation of myocardial ischemia reperfusion injury. Mol Med Rep 25: 176, 2022.
APA
Zhan, J., Yin, Q., Zhao, P., & Hong, L. (2022). Role and mechanism of the lncRNA SNHG1/miR‑450b‑5p/IGF1 axis in the regulation of myocardial ischemia reperfusion injury. Molecular Medicine Reports, 25, 176. https://doi.org/10.3892/mmr.2022.12692
MLA
Zhan, J., Yin, Q., Zhao, P., Hong, L."Role and mechanism of the lncRNA SNHG1/miR‑450b‑5p/IGF1 axis in the regulation of myocardial ischemia reperfusion injury". Molecular Medicine Reports 25.5 (2022): 176.
Chicago
Zhan, J., Yin, Q., Zhao, P., Hong, L."Role and mechanism of the lncRNA SNHG1/miR‑450b‑5p/IGF1 axis in the regulation of myocardial ischemia reperfusion injury". Molecular Medicine Reports 25, no. 5 (2022): 176. https://doi.org/10.3892/mmr.2022.12692
Copy and paste a formatted citation
x
Spandidos Publications style
Zhan J, Yin Q, Zhao P and Hong L: Role and mechanism of the lncRNA SNHG1/miR‑450b‑5p/IGF1 axis in the regulation of myocardial ischemia reperfusion injury. Mol Med Rep 25: 176, 2022.
APA
Zhan, J., Yin, Q., Zhao, P., & Hong, L. (2022). Role and mechanism of the lncRNA SNHG1/miR‑450b‑5p/IGF1 axis in the regulation of myocardial ischemia reperfusion injury. Molecular Medicine Reports, 25, 176. https://doi.org/10.3892/mmr.2022.12692
MLA
Zhan, J., Yin, Q., Zhao, P., Hong, L."Role and mechanism of the lncRNA SNHG1/miR‑450b‑5p/IGF1 axis in the regulation of myocardial ischemia reperfusion injury". Molecular Medicine Reports 25.5 (2022): 176.
Chicago
Zhan, J., Yin, Q., Zhao, P., Hong, L."Role and mechanism of the lncRNA SNHG1/miR‑450b‑5p/IGF1 axis in the regulation of myocardial ischemia reperfusion injury". Molecular Medicine Reports 25, no. 5 (2022): 176. https://doi.org/10.3892/mmr.2022.12692
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