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Preparation of Nereid oligopeptide and investigation of the mechanism underlying the induction of apoptosis in human lung cancer H1299 cells

  • Authors:
    • Guomei Zhang
    • Han Li
    • Shanshan Liu
    • Mingyang Lu
    • Liang Tang
    • Lihua Sun
  • View Affiliations / Copyright

    Affiliations: College of Food Science and Engineering, Hangzhou Medical College, Hangzhou, Zhejiang 310013, P.R. China
    Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 194
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    Published online on: April 14, 2022
       https://doi.org/10.3892/mmr.2022.12710
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Abstract

In the present study, oligopeptides from Nereid (Perinereis aibuhitensis) were prepared via enzymatic hydrolysis, and the mechanism underlying the induction of apoptosis in H1299 cells was investigated. According to the analysis of the inhibition rate on proliferation, alkaline protease demonstrated the best enzymatic efficiency. The optimal conditions for hydrolysis were as follows: 50˚C and pH 10 for 6 h; a material‑to‑liquid ratio of 1:1 (g/ml); and addition of 400 U/g enzyme. The hydrolysates were purified using ultrafiltration, anion chromatography, gel filtration chromatography, and high‑performance liquid chromatography. The Nereid oligopeptide (NOP), with a molecular weight of 841 kDa and an amino acid sequence of glutamine‑isoleucine‑asparagine‑glutamine‑histidine‑leucine, was obtained. NOP inhibited the proliferation of H1299 cells in a time‑ and dose‑dependent manner. Morphological changes and apoptosis were also induced by NOP in H1299 cells. The western blot analysis revealed that the B‑cell lymphoma 2/Bcl‑2 associated X (Bcl‑2/Bax) ratio was reduced by 24.7% in the NOP treatment group compared with the control group. The relative expression levels of cleaved caspase‑9 (cleaved‑CASP9) and cleaved caspase‑3 (cleaved‑CASP3) in the NOP treatment group were 2.55‑ and 1.71‑fold higher than those measured in the control group, respectively. These results suggested that NOP exerts antitumor effects by influencing the proliferation and apoptosis of H1299 cells.
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View References

1 

Guo Q: Current situation of medical treatment of non-small cell lung cancer. Chin J Cancer Prev Treat. 16:721–725. 2009.

2 

Ahmed S, Mirzaei H, Aschner M, Khan A, Al-Harrasi A and Khan H: Marine peptides in breast cancer: Therapeutic and mechanistic understanding. Biomed Pharmacother. 142:1120382021. View Article : Google Scholar : PubMed/NCBI

3 

Shi F, Yan X, Cheong KL and Liu Y: Extraction, purification, and characterization of polysaccharides from marine algae gracilaria lemaneiformis with anti-tumor activity. Process Biochem. 73:197–203. 2018. View Article : Google Scholar

4 

Huang ZZ, Du X, Ma CD, Zhang RR, Gong WL and Liu F: Identification of antitumor active constituents in polygonatum sibiricum flower by UPLC-Q-TOF-MSE and network pharmacology. ACS Omega. 5:29755–29764. 2020. View Article : Google Scholar : PubMed/NCBI

5 

Margolin K, Longmate J, Synold TW, Gandara DR, Weber J, Gonzalez R, Johansen MJ, Newman R, Baratta T and Doroshow JH: Dolastatin-10 in metastatic melanoma: A phase II and pharmokinetic trial of the California cancer consortium. Invest New Drugs. 19:335–340. 2001. View Article : Google Scholar : PubMed/NCBI

6 

Takahashi T, Furukawa Y, Muneoka Y, Matsushima O, Ikeda T, Fujita T, Minakata H and Nomoto K: Isolation and characterization of four novel bioactive peptides from a polychaete annelid, Perinereis vancaurica. Comp Biochem Physiol C Pharmacol Toxicol Endocrinol. 110:297–304. 1995. View Article : Google Scholar : PubMed/NCBI

7 

LI Q: Purification, characterization and pharmacodynamic activity of proteases and its isoenzyme from Nereis virens (unpublished PhD thesis). Jilin University; 2008

8 

Bai R, Li Q, Liu J, Jiang X and Hong M: Effects of Nereis virens proteinase on platelet aggregation and hemorheology. Chin J New Drugs. 18:930–933. 2009.(In Chinese).

9 

Bo Q, Ge X, Cui J, Jiang X, Liu J and Hong M: Effects of acidic serine protease ASPNJ on inhibition and injury of leukemia cell K562. Chin J Biochem Pharm. 33:736–739. 2012.(In Chinese).

10 

Ge X, Bo Q, Hong X, Cui J, Jiang X, Hong M and Liu J: A novel acidic serine protease, ASPNJ inhibits proliferation, induces apoptosis and enhances chemo-susceptibility of acute promyelocytic leukemia cell. Leuk Res. 37:1697–1703. 2013. View Article : Google Scholar : PubMed/NCBI

11 

McNair K, Forrest CM, Vincenten M, Darlington LG and Stone TW: Serine protease modulation of dependence receptors and EMT protein expression. Cancer Biol Ther. 20:349–367. 2019. View Article : Google Scholar : PubMed/NCBI

12 

Zhang G, Yang M, Ding G, Huang F and Zhao Y: Study on the mechanism of apoptosis induced by SPC-A-1 cells in human lung cancer. Mod Food Technol. 31:6–11. 2015.

13 

Yoon SK, Sung SK, Lee DH and Kim HW: Tissue inhibitor of metalloproteinase-1 (TIMP-1) and IL-23 induced by polysaccharide of the black hoof medicinal mushroom, phellinus linteus (Agaricomycetes). Int J Med Mushrooms. 19:213–223. 2017. View Article : Google Scholar : PubMed/NCBI

14 

Pan W, Liu X, Ge F, Han J and Zheng T: Perinerin, a novel antimicrobial peptide purified from the clamworm Perinereis aibuhitensis grube and its partial characterization. J Biochem. 135:297–304. 2004. View Article : Google Scholar : PubMed/NCBI

15 

Chen X, Luo Y, Qi B, Luo J and Wan Y: Improving the hydrolysis efficiency of soy sauce residue using ultrasonic probe-assisted enzymolysis technology. Ultrason Sonochem. 35:351–358. 2017. View Article : Google Scholar : PubMed/NCBI

16 

Jiang Z, Xu Y and Su Y: Preparation process of active enzymolysis polypeptides from seahorse bone meal. Food Sci Nutr. 2:490–499. 2014. View Article : Google Scholar : PubMed/NCBI

17 

Qu W, Ma H, Jia J, He R, Luo L and Pan Z: Enzymolysis kinetics and activities of ACE inhibitory peptides from wheat germ protein prepared with SFP ultrasound-assisted processing. Ultrason Sonochem. 19:1021–1026. 2012. View Article : Google Scholar : PubMed/NCBI

18 

Kitts DD and Weiler K: Bioactive proteins and peptides from food sources. Applications of bioprocesses used in isolation and recovery. Curr Pharm Des. 9:1309–1023. 2003. View Article : Google Scholar : PubMed/NCBI

19 

Qian B, Zhao X, Yang Y and Tian C: Antioxidant and anti-inflammatory peptide fraction from oyster soft tissue by enzymatic hydrolysis. Food Sci Nutr. 8:3947–3956. 2020. View Article : Google Scholar : PubMed/NCBI

20 

Maqsoudlou A, Sadeghi MA, Mora L, Mohebodini H, Ghorbani M and Toldrá F: Controlled enzymatic hydrolysis of pollen protein as promising tool for production of potential bioactive peptides. J Food Biochem. 43:e128192019. View Article : Google Scholar : PubMed/NCBI

21 

Zhou J: Fucoidan extraction, purification, structure analysis and antioxidant research (unpublished PhD thesis). Hangzhou: Zhejiang University; 2006

22 

Zhu S, Chen M, Niu Q, Li T, Qu Y and Chen Y: Physico-chemical properties and structure analysis of viscera polysaccharide of urechis unicinctus and its lipid peroxidation inhibition activity. Food Sci. 36:67–71. 2015.(In Chinese).

23 

Huang F, Yang Z, Yu D, Wang J, Li R and Ding G: Sepia ink oligopeptide induces apoptosis in prostate cancer cell lines via caspase-3 activation and elevation of Bax/Bcl-2 ratio. Mar Drugs. 10:2153–2165. 2012. View Article : Google Scholar : PubMed/NCBI

24 

Ding G, Huang F, Yang Z, Yu D and Yang Y: Anticancer activity of an oligopeptide isolated from hydrolysates of sepia ink. Chin J Nat Med. 9:151–155. 2011.

25 

Grela E, Kozłowska J and Grabowiecka A: Current methodology of MTT assay in bacteria-a review. Acta Histochem. 120:303–311. 2018. View Article : Google Scholar : PubMed/NCBI

26 

Ma Y, Zhou B, Sun X, Zhang L, Song L and Xiao H: The effect of diosone active component on the proliferation of glomerular mesangial cells was determined by MTT assay. Tradit Chin Med Inf. 27:34–36. 2010.

27 

Chen M, Ge A, Cui P and Liao Z: Study on inhibition effects of phycoerythrin from Gracilaria lemaneiformis on Hela cells and its mechanism. Food Sci. 28:549–552. 2007.(In Chinese).

28 

Pettit GR, Kamano Y, Herald CL, Tuinman AA, Boettner FE, Kizu H, Schmidt JM, Baczynskyj L, Tomer KB and Bontems RJ: The isolation and structure of a remarkable marine animal antineoplastic constituent: Dolastatin 10. J Am Chem Soc. 109:6883–6885. 1987. View Article : Google Scholar

29 

Cao W and Song J: Progress in the antitumor polypeptide Aplysia toxin 10 and its derivatives of marine organisms. Grad Med J. 24:1208–1211. 2011.(In Chinese).

30 

Shnyder SD, Cooper PA, Millington NJ, Pettit GR and Bibby MC: Auristatin PYE, a novel synthetic derivative of dolastatin 10, is highly effective in human colon tumour models. Int J Oncol. 31:353–360. 2007.PubMed/NCBI

31 

Chi CF, Hu FY, Wang B, Li T and Ding GF: Antioxidant and anticancer peptides from the protein hydrolysate of blood clam (Tegillarcagranosa) muscle. J Funct Foods. 15:301–313. 2015. View Article : Google Scholar

32 

Yao R Chu X, Chen S, Wang C and Liu W: Experimental study on the antitumor effects of polypeptides. Chin Pharm J. 41:868–870. 2006.

33 

Wang C, Liu M, Wang F and Lin X: Growth-inhibition effects of a novel anti-tumor polypeptide from Meretrix meretrix Linnaeus associated with tubulin polymerization. Chin J Biochem Pharm. 33:225–228. 2012.(In Chinese).

34 

Zhu X: Progress in studying the morphological characteristics and molecular mechanisms of cell apoptosis. Chin J Gerontol. 31:2595–2597. 2011.(In Chinese).

35 

Chio S, Lew KL, Xiao H, Herman-Antosiewicz A, Xiao D, Brown CK and Singh SV: D,L-Sulforaphane-induced cell death in human prostate cancer cells is regulated by inhibitor of apoptosis family proteins and Apaf-1. Carcinogenesis. 28:151–162. 2007. View Article : Google Scholar : PubMed/NCBI

36 

Clark CS and Maurelli AT: Shigella flexneri inhibits staurosporine-induced apoptosis in epithelial cells. Infect Immun. 75:2531–2539. 2007. View Article : Google Scholar : PubMed/NCBI

37 

Chen ZQ, Jie X and Mo ZN: Curcumin inhibits growth, induces G1 arrest and apoptosis on human prostatic stromal cells by regulating Bcl-2/Bax. Zhongguo Zhong Yao Za Zhi. 33:2022–2025. 2008.(In Chinese). PubMed/NCBI

38 

Liu W, Hμang XF, Qi Q, Dai QS, Yang L, Nie FF, Lu N, Gong DD, Kong LY and Guo QL: Asparanin A induces G(2)/M cell cycle arrest and apoptosis in human hepatocellular carcinoma HepG2 cells. Biochem Biophys Res Commun. 381:700–705. 2009. View Article : Google Scholar : PubMed/NCBI

39 

Xu X, Liu Y, Wang L, He J, Zhang H, Chen X, Li Y, Yang J and Tao J: Gambogic acid induces apoptosis by regulating the expression of Bax and Bcl-2 and enhancing caspase-3 activity in human malignant melanoma A375 cells. Int J Dermatol. 48:186–192. 2009. View Article : Google Scholar : PubMed/NCBI

40 

Bhattacharjee M, Acharya S, Ghosh A, Sarkar P, Chatterjee S, Kumar P and Chaudhuri S: Bax and Bid act in synergy to bring about T11TS-mediated glioma apoptosis via the release of mitochondrial cytochrome c and subsequent caspase activation. Int Immunol. 20:1489–1505. 2008. View Article : Google Scholar : PubMed/NCBI

41 

Liu HR, Peng XD, He HB, Wang YH, Li Y, He GX, Liu YL, Li YL and Zeng CJ: Antiproliferative activity of the total saponin of solanum lyratum thunb in Hela cells by inducing apoptosis. Pharmazie. 63:836–842. 2008.PubMed/NCBI

42 

Bressenot A, Marchal S, Bezdetnaya L, Garrier J, Guillemin F and Plénat F: Assessment of apoptosis by immunohistochemistry to active caspase-3, active caspase-7, or cleaved PARP in monolayer cells and spheroid and subcutaneous xenografts of human carcinoma. J Histochem Cytochem. 57:289–300. 2009. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Zhang G, Li H, Liu S, Lu M, Tang L and Sun L: Preparation of Nereid oligopeptide and investigation of the mechanism underlying the induction of apoptosis in human lung cancer H1299 cells. Mol Med Rep 25: 194, 2022.
APA
Zhang, G., Li, H., Liu, S., Lu, M., Tang, L., & Sun, L. (2022). Preparation of Nereid oligopeptide and investigation of the mechanism underlying the induction of apoptosis in human lung cancer H1299 cells. Molecular Medicine Reports, 25, 194. https://doi.org/10.3892/mmr.2022.12710
MLA
Zhang, G., Li, H., Liu, S., Lu, M., Tang, L., Sun, L."Preparation of Nereid oligopeptide and investigation of the mechanism underlying the induction of apoptosis in human lung cancer H1299 cells". Molecular Medicine Reports 25.6 (2022): 194.
Chicago
Zhang, G., Li, H., Liu, S., Lu, M., Tang, L., Sun, L."Preparation of Nereid oligopeptide and investigation of the mechanism underlying the induction of apoptosis in human lung cancer H1299 cells". Molecular Medicine Reports 25, no. 6 (2022): 194. https://doi.org/10.3892/mmr.2022.12710
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang G, Li H, Liu S, Lu M, Tang L and Sun L: Preparation of Nereid oligopeptide and investigation of the mechanism underlying the induction of apoptosis in human lung cancer H1299 cells. Mol Med Rep 25: 194, 2022.
APA
Zhang, G., Li, H., Liu, S., Lu, M., Tang, L., & Sun, L. (2022). Preparation of Nereid oligopeptide and investigation of the mechanism underlying the induction of apoptosis in human lung cancer H1299 cells. Molecular Medicine Reports, 25, 194. https://doi.org/10.3892/mmr.2022.12710
MLA
Zhang, G., Li, H., Liu, S., Lu, M., Tang, L., Sun, L."Preparation of Nereid oligopeptide and investigation of the mechanism underlying the induction of apoptosis in human lung cancer H1299 cells". Molecular Medicine Reports 25.6 (2022): 194.
Chicago
Zhang, G., Li, H., Liu, S., Lu, M., Tang, L., Sun, L."Preparation of Nereid oligopeptide and investigation of the mechanism underlying the induction of apoptosis in human lung cancer H1299 cells". Molecular Medicine Reports 25, no. 6 (2022): 194. https://doi.org/10.3892/mmr.2022.12710
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