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Article Open Access

CXCL16/ERK1/2 pathway regulates human podocytes growth, migration, apoptosis and epithelial mesenchymal transition

  • Authors:
    • Yuan Chen
    • Zhiyi Wang
    • Qian Li
    • Minle Tian
    • Yanji Zhu
    • Lichun Yu
    • Jing Wang
    • Shuzhen Sun
  • View Affiliations / Copyright

    Affiliations: Department of Pediatrics, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong 250021, P.R. China, Department of Hepatobiliary Surgery, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong 250021, P.R. China, Department of Pediatrics, People's Hospital of Rizhao, Rizhao, Shandong 276800, P.R. China
    Copyright: © Chen et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 212
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    Published online on: May 4, 2022
       https://doi.org/10.3892/mmr.2022.12728
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Abstract

Primary nephrotic syndrome (PNS) is the commonest glomerular disease affecting children. Previous studies have confirmed that CXC motif chemokine ligand 16 (CXCL16) is involved in the pathogenesis of PNS. However, the exact mechanisms underlying the pathogenesis of PNS remain to be elucidated. Thus, the present study aimed to elucidate the role of CXCL16 in PNS. It was found that the expression of CXCL16 and extracellular signal‑regulated kinases 1 and 2 (ERK1/2) were significantly increased in clinical PNS renal tissues using reverse transcription‑quantitative PCR, western blot analysis and immunohistochemistry. Lentivirus overexpression or short hairpin RNA vector was used to induce the overexpression or knockdown of CXCL16 in podocytes, respectively. Overexpression of CXCL16 in podocytes could decrease the cell proliferation and increase the migration and apoptosis, whereas CXCL16 knockdown increased cell proliferation and decreased cell migration and apoptosis. Results of the present study further demonstrated that ERK2 protein expression was regulated by CXCL16. The knockdown of ERK2 expression reversed the effects of CXCL16 on the proliferation, apoptosis, migration and epithelial mesenchymal transition (EMT) of podocytes. Collectively, the findings of the present study highlighted that the CXCL16/ERK1/2 pathway regulates the growth, migration, apoptosis and EMT of human podocytes.
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Copy and paste a formatted citation
Spandidos Publications style
Chen Y, Wang Z, Li Q, Tian M, Zhu Y, Yu L, Wang J and Sun S: CXCL16/ERK1/2 pathway regulates human podocytes growth, migration, apoptosis and epithelial mesenchymal transition. Mol Med Rep 25: 212, 2022.
APA
Chen, Y., Wang, Z., Li, Q., Tian, M., Zhu, Y., Yu, L. ... Sun, S. (2022). CXCL16/ERK1/2 pathway regulates human podocytes growth, migration, apoptosis and epithelial mesenchymal transition. Molecular Medicine Reports, 25, 212. https://doi.org/10.3892/mmr.2022.12728
MLA
Chen, Y., Wang, Z., Li, Q., Tian, M., Zhu, Y., Yu, L., Wang, J., Sun, S."CXCL16/ERK1/2 pathway regulates human podocytes growth, migration, apoptosis and epithelial mesenchymal transition". Molecular Medicine Reports 25.6 (2022): 212.
Chicago
Chen, Y., Wang, Z., Li, Q., Tian, M., Zhu, Y., Yu, L., Wang, J., Sun, S."CXCL16/ERK1/2 pathway regulates human podocytes growth, migration, apoptosis and epithelial mesenchymal transition". Molecular Medicine Reports 25, no. 6 (2022): 212. https://doi.org/10.3892/mmr.2022.12728
Copy and paste a formatted citation
x
Spandidos Publications style
Chen Y, Wang Z, Li Q, Tian M, Zhu Y, Yu L, Wang J and Sun S: CXCL16/ERK1/2 pathway regulates human podocytes growth, migration, apoptosis and epithelial mesenchymal transition. Mol Med Rep 25: 212, 2022.
APA
Chen, Y., Wang, Z., Li, Q., Tian, M., Zhu, Y., Yu, L. ... Sun, S. (2022). CXCL16/ERK1/2 pathway regulates human podocytes growth, migration, apoptosis and epithelial mesenchymal transition. Molecular Medicine Reports, 25, 212. https://doi.org/10.3892/mmr.2022.12728
MLA
Chen, Y., Wang, Z., Li, Q., Tian, M., Zhu, Y., Yu, L., Wang, J., Sun, S."CXCL16/ERK1/2 pathway regulates human podocytes growth, migration, apoptosis and epithelial mesenchymal transition". Molecular Medicine Reports 25.6 (2022): 212.
Chicago
Chen, Y., Wang, Z., Li, Q., Tian, M., Zhu, Y., Yu, L., Wang, J., Sun, S."CXCL16/ERK1/2 pathway regulates human podocytes growth, migration, apoptosis and epithelial mesenchymal transition". Molecular Medicine Reports 25, no. 6 (2022): 212. https://doi.org/10.3892/mmr.2022.12728
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