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Article Open Access

A novel 7‑hypoxia‑related long non‑coding RNA signature associated with prognosis and proliferation in melanoma

  • Authors:
    • Yi Luo
    • Tinghao Li
    • Hengguang Zhao
    • Aijun Chen
  • View Affiliations / Copyright

    Affiliations: Department of Dermatology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, P.R. China, Department of Urology, The First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, P.R. China, Department of Dermatology, The Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, P.R. China
    Copyright: © Luo et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 255
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    Published online on: June 14, 2022
       https://doi.org/10.3892/mmr.2022.12771
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Abstract

Hypoxia‑related long non‑coding RNAs (lncRNAs) are important indicators of the poor prognosis of cancers. The present study aimed to explore the potential relationship between melanoma and hypoxia‑related lncRNAs. The transcriptome and clinical data of patients with melanoma were downloaded from The Cancer Genome Atlas database. The prognostic hypoxia‑related lncRNAs were screened out using Pearson's correlation test and univariate Cox analysis. As a result, a hypoxia‑related‑lncRNA signature based on the expression of 7 lncRNAs was constructed, with one unfavourable [MIR205 host gene (MIR205HG)] and six favourable (T cell receptor β variable 11‑2, HLA‑DQB1 antisense RNA 1, AL365361.1, AC004847.1, ubiquitin specific peptidase 30 antisense RNA 1 and AC022706.1) lncRNAs as prognostic factors for melanoma. Patients with melanoma were divided into high‑ and low‑risk groups based on the risk score obtained. Survival analyses were performed to assess the prognostic value of the present risk model. Potential tumour‑associated biological pathways associated with the present signature were explored using gene set enrichment analysis. The CIBERSORT algorithm demonstrated the important role of the hypoxia‑related lncRNAs in regulating tumour‑infiltrating immune cells. Clinical samples collected from our center partly confirmed our findings. Cell Counting Kit‑8 and flow cytometry assays indicated the suppression of proliferation of melanoma cells following inhibition of MIR205HG expression. Indicators of the canonical Wnt/β‑catenin signalling pathway were detected by western blotting. The present study demonstrated that MIR205HG could promote melanoma cell proliferation partly via the canonical Wnt/β‑catenin signalling pathway. These findings indicated a 7‑hypoxia‑related‑lncRNA signature that can serve as a novel predictor of melanoma prognosis.
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Copy and paste a formatted citation
Spandidos Publications style
Luo Y, Li T, Zhao H and Chen A: A novel 7‑hypoxia‑related long non‑coding RNA signature associated with prognosis and proliferation in melanoma. Mol Med Rep 26: 255, 2022.
APA
Luo, Y., Li, T., Zhao, H., & Chen, A. (2022). A novel 7‑hypoxia‑related long non‑coding RNA signature associated with prognosis and proliferation in melanoma. Molecular Medicine Reports, 26, 255. https://doi.org/10.3892/mmr.2022.12771
MLA
Luo, Y., Li, T., Zhao, H., Chen, A."A novel 7‑hypoxia‑related long non‑coding RNA signature associated with prognosis and proliferation in melanoma". Molecular Medicine Reports 26.2 (2022): 255.
Chicago
Luo, Y., Li, T., Zhao, H., Chen, A."A novel 7‑hypoxia‑related long non‑coding RNA signature associated with prognosis and proliferation in melanoma". Molecular Medicine Reports 26, no. 2 (2022): 255. https://doi.org/10.3892/mmr.2022.12771
Copy and paste a formatted citation
x
Spandidos Publications style
Luo Y, Li T, Zhao H and Chen A: A novel 7‑hypoxia‑related long non‑coding RNA signature associated with prognosis and proliferation in melanoma. Mol Med Rep 26: 255, 2022.
APA
Luo, Y., Li, T., Zhao, H., & Chen, A. (2022). A novel 7‑hypoxia‑related long non‑coding RNA signature associated with prognosis and proliferation in melanoma. Molecular Medicine Reports, 26, 255. https://doi.org/10.3892/mmr.2022.12771
MLA
Luo, Y., Li, T., Zhao, H., Chen, A."A novel 7‑hypoxia‑related long non‑coding RNA signature associated with prognosis and proliferation in melanoma". Molecular Medicine Reports 26.2 (2022): 255.
Chicago
Luo, Y., Li, T., Zhao, H., Chen, A."A novel 7‑hypoxia‑related long non‑coding RNA signature associated with prognosis and proliferation in melanoma". Molecular Medicine Reports 26, no. 2 (2022): 255. https://doi.org/10.3892/mmr.2022.12771
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