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Upregulation of SIRT1 by Evodiamine activates PI3K/AKT pathway and blocks intervertebral disc degeneration

  • Authors:
    • Jianbo Kuai
    • Na Zhang
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedics, Jianhu County People's Hospital, Yancheng, Jiangsu 224700, P.R. China, Department of Orthopedics, Fengfeng General Hospital, North China Medical Health Group, Handan, Hebei 056200, P.R. China
    Copyright: © Kuai et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 265
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    Published online on: June 24, 2022
       https://doi.org/10.3892/mmr.2022.12781
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Abstract

Intervertebral disc degeneration (IDD) is a major cause of a number of spinal diseases, resulting in serious public health problems. Evodiamine (Evo) is an indole quinazoline alkaloid extracted from Evodia rutaecarpa, which has antioxidant, anti‑apoptosis and anti‑inflammatory effects. The purpose of the present study was to investigate lipopolysaccharide (LPS)‑induced IDD progression in human nucleus pulposus cells (NPCs) and its potential mechanism. The viability and apoptosis of NPCs were detected by Cell Counting Kit‑8 (CCK‑8) and TUNEL staining, respectively. Western blotting was used to detect the expression levels of proteins, cell transfection was performed to knockdown Sirtuin 1 (SIRT1) and the expression of tumor necrosis factor‑alpha (TNF‑α) and interleukin 6 (IL‑6) was detected by enzyme‑linked immunosorbent assay kits. The results showed that Evo effectively alleviated LPS‑induced NPCs apoptosis and caspase‑3 activation and Evo treatment reversed the upregulation of matrix metalloproteinase‑13, as well as the downregulation of collagen type II (collagen II), Sry‑type high‑mobility‑group box 9 and aggrecan and reduced the production of pro‑inflammatory factors TNF‑α and IL‑6 in LPS‑stimulated NPCs. In addition, treatment with Evo upregulated SIRT1 and activated the PI3K/Akt pathway, knockdown of SIRT1 inhibited the phosphorylation of Akt and PI3K in LPS‑stimulated NPCs. In general, Evo upregulated SIRT1 and inhibited LPS‑induced NPCs apoptosis, extracellular matrix degradation and inflammation by activating the PI3K/Akt pathway.
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1 

Vergroesen PP, Kingma I, Emanuel KS, Hoogendoorn RJ, Welting TJ, van Royen BJ, van Dieën JH and Smit TH: Mechanics and biology in intervertebral disc degeneration: A vicious circle. Osteoarthritis Cartilage. 23:1057–1070. 2015. View Article : Google Scholar : PubMed/NCBI

2 

Risbud MV and Shapiro IM: Role of cytokines in intervertebral disc degeneration: Pain and disc content. Nat Rev Rheumatol. 10:44–56. 2014. View Article : Google Scholar : PubMed/NCBI

3 

Kos N, Gradisnik L and Velnar T: A brief review of the degenerative intervertebral disc disease. Med Arch. 73:421–424. 2019. View Article : Google Scholar : PubMed/NCBI

4 

González Martínez E, García-Cosamalón J, Cosamalón-Gan I, Esteban Blanco M, García-Suarez O and Vega JA: Biology and mechanobiology of the intervertebral disc. Neurocirugia (Astur). 28:135–140. 2017.(In Spanish). View Article : Google Scholar : PubMed/NCBI

5 

Liao Z, Luo R, Li G, Song Y, Zhan S, Zhao K, Hua W, Zhang Y, Wu X and Yang C: Exosomes from mesenchymal stem cells modulate endoplasmic reticulum stress to protect against nucleus pulposus cell death and ameliorate intervertebral disc degeneration in vivo. Theranostics. 9:4084–4100. 2019. View Article : Google Scholar : PubMed/NCBI

6 

Cheng X, Zhang G, Zhang L, Hu Y, Zhang K, Sun X, Zhao C, Li H, Li YM and Zhao J: Mesenchymal stem cells deliver exogenous miR-21 via exosomes to inhibit nucleus pulposus cell apoptosis and reduce intervertebral disc degeneration. J Cell Mol Med. 22:261–276. 2018. View Article : Google Scholar : PubMed/NCBI

7 

Molinos M, Almeida CR, Caldeira J, Cunha C, Gonçalves RM and Barbosa MA: Inflammation in intervertebral disc degeneration and regeneration. J R Soc Interface. 12:201504292015. View Article : Google Scholar : PubMed/NCBI

8 

Li X, Ge J, Zheng Q, Zhang J, Sun R and Liu R: Evodiamine and rutaecarpine from Tetradium ruticarpum in the treatment of liver diseases. Phytomedicine. 68:1531802020. View Article : Google Scholar : PubMed/NCBI

9 

Xu D, Qiu C, Wang Y, Qiao T and Cui YL: Intranasal co-delivery of berberine and evodiamine by self-assembled thermosensitive in-situ hydrogels for improving depressive disorder. Int J Pharm. 603:1206672021. View Article : Google Scholar : PubMed/NCBI

10 

Wang MX, Lin L, Chen YD, Zhong YP, Lin YX, Li P, Tian X, Han B, Xie ZY and Liao QF: Evodiamine has therapeutic efficacy in ulcerative colitis by increasing Lactobacillus acidophilus levels and acetate production. Pharmacol Res. 159:1049782020. View Article : Google Scholar : PubMed/NCBI

11 

Li CG, Zeng QZ, Chen MY, Xu LH, Zhang CC, Mai FY, Zeng CY, He XH and Ouyang DY: Evodiamine augments NLRP3 inflammasome activation and anti-bacterial responses through inducing α-tubulin acetylation. Front Pharmacol. 10:2902019. View Article : Google Scholar : PubMed/NCBI

12 

Zhang WD, Chen XY, Wu C, Lian YN, Wang YJ, Wang JH, Yang F, Liu CH and Li XY: Evodiamine reduced peripheral hypersensitivity on the mouse with nerve injury or inflammation. Mol Pain. 16:17448069209025632020. View Article : Google Scholar : PubMed/NCBI

13 

Wang Y, Yang W, Peng F, Chen T, Fu Y and Tian M: Evodiamine inhibits injury of HUVECs induced by lipopolysaccharide through TLR4/NF-κB signaling pathway. Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 35:1088–1093. 2019.(In Chinese). PubMed/NCBI

14 

Shi Y, Hua Q, Li N, Zhao M and Cui Y: Protective effects of evodiamine against LPS-induced acute kidney injury through regulation of ROS-NF-κB-mediated inflammation. Evid Based Complement Alternat Med. 2019:21908472019. View Article : Google Scholar : PubMed/NCBI

15 

Guo J, Shao M, Lu F, Jiang J and Xia X: Role of Sirt1 plays in nucleus pulposus cells and intervertebral disc degeneration. Spine (Phila Pa 1976). 42:E757–E766. 2017. View Article : Google Scholar : PubMed/NCBI

16 

Xia X, Guo J, Lu F and Jiang J: SIRT1 plays a protective role in intervertebral disc degeneration in a puncture-induced rodent model. Spine (Phila Pa 1976). 40:E515–E524. 2015. View Article : Google Scholar : PubMed/NCBI

17 

Zhou P, Li XP, Jiang R, Chen Y, Lv XT, Guo XX, Tian K, Yuan DZ, Lv YW, Ran JH, et al: Evodiamine inhibits migration and invasion by Sirt1-mediated post-translational modulations in colorectal cancer. Anticancer Drugs. 30:611–617. 2019. View Article : Google Scholar : PubMed/NCBI

18 

Wang C, Wang MW, Tashiro S, Onodera S and Ikejima T: Roles of SIRT1 and phosphoinositide 3-OH kinase/protein kinase C pathways in evodiamine-induced human melanoma A375-S2 cell death. J Pharmacol Sci. 97:494–500. 2005. View Article : Google Scholar : PubMed/NCBI

19 

Ouyang ZH, Wang WJ, Yan YG, Wang B and Lv GH: The PI3K/Akt pathway: A critical player in intervertebral disc degeneration. Oncotarget. 8:57870–57881. 2017. View Article : Google Scholar : PubMed/NCBI

20 

Tan Y, Yao X, Dai Z, Wang Y and Lv G: Bone morphogenetic protein 2 alleviated intervertebral disc degeneration through mediating the degradation of ECM and apoptosis of nucleus pulposus cells via the PI3K/Akt pathway. Int J Mol Med. 43:583–592. 2019.PubMed/NCBI

21 

Guo HT, Yang SD, Zhang F, Liu S, Yang DL, Ma L, Wang H and Ding WY: 17β-Estradiol protects against interleukin-1β-induced apoptosis in rat nucleus pulposus cells via the mTOR/caspase-3 pathway. Mol Med Rep. 20:1523–1530. 2019.PubMed/NCBI

22 

Ren BC, Zhang YF, Liu SS, Cheng XJ, Yang X, Cui XG, Zhao XR, Zhao H, Hao MF, Li MD, et al: Curcumin alleviates oxidative stress and inhibits apoptosis in diabetic cardiomyopathy via Sirt1-Foxo1 and PI3K-Akt signalling pathways. J Cell Mol Med. 24:12355–12367. 2020. View Article : Google Scholar : PubMed/NCBI

23 

Qi W, Ren D, Wang P, Song Z, Wu H, Yao S, Geng L, Su Y and Bai X: Upregulation of Sirt1 by tyrosol suppresses apoptosis and inflammation and modulates extracellular matrix remodeling in interleukin-1β-stimulated human nucleus pulposus cells through activation of PI3K/Akt pathway. Int Immunopharmacol. 88:1069042020. View Article : Google Scholar : PubMed/NCBI

24 

Brinjikji W, Diehn FE, Jarvik JG, Carr CM, Kallmes DF, Murad MH and Luetmer PH: MRI findings of disc degeneration are more prevalent in adults with low back pain than in asymptomatic controls: A systematic review and meta-analysis. AJNR Am J Neuroradiol. 36:2394–2399. 2015. View Article : Google Scholar : PubMed/NCBI

25 

Cazzanelli P and Wuertz-Kozak K: MicroRNAs in intervertebral disc degeneration, apoptosis, inflammation, and mechanobiology. Int J Mol Sci. 21:36012020. View Article : Google Scholar : PubMed/NCBI

26 

Xiang Q, Kang L, Wang J, Liao Z, Song Y, Zhao K, Wang K, Yang C and Zhang Y: CircRNA-CIDN mitigated compression loading-induced damage in human nucleus pulposus cells via miR-34a-5p/SIRT1 axis. EBioMedicine. 53:1026792020. View Article : Google Scholar : PubMed/NCBI

27 

Guo HY, Guo MK, Wan ZY, Song F and Wang HQ: Emerging evidence on noncoding-RNA regulatory machinery in intervertebral disc degeneration: A narrative review. Arthritis Res Ther. 22:2702020. View Article : Google Scholar : PubMed/NCBI

28 

Xue Y, Guo T, Zou L, Gong Y, Wu B, Yi Z, Jia T, Zhao S, Shi L, Li L, et al: Evodiamine attenuates P2X7-mediated inflammatory injury of human umbilical vein endothelial cells exposed to high free fatty acids. Oxid Med Cell Longev. 2018:50828172018. View Article : Google Scholar : PubMed/NCBI

29 

Hu YY, He KW and Zhu HD: Chinese herbal medicinal ingredients affect secretion of NO, IL-10, ICAM-1 and IL-2 by endothelial cells. Immunopharmacol Immunotoxicol. 37:324–328. 2015. View Article : Google Scholar : PubMed/NCBI

30 

Tang BL: Sirt1 and the mitochondria. Mol Cells. 39:87–95. 2016. View Article : Google Scholar : PubMed/NCBI

31 

Jiao F and Gong Z: The beneficial roles of SIRT1 in neuroinflammation-related diseases. Oxid Med Cell Longev. 2020:67828722020. View Article : Google Scholar : PubMed/NCBI

32 

Xie L, Huang W, Fang Z, Ding F, Zou F, Ma X, Tao J, Guo J, Xia X, Wang H, et al: CircERCC2 ameliorated intervertebral disc degeneration by regulating mitophagy and apoptosis through miR-182-5p/SIRT1 axis. Cell Death Dis. 10:7512019. View Article : Google Scholar : PubMed/NCBI

33 

Xie Y, Shi X, Sheng K, Han G, Li W, Zhao Q, Jiang B, Feng J, Li J and Gu Y: PI3K/Akt signaling transduction pathway, erythropoiesis and glycolysis in hypoxia (review). Mol Med Rep. 19:783–791. 2019.PubMed/NCBI

34 

Pompura SL and Dominguez-Villar M: The PI3K/AKT signaling pathway in regulatory T-cell development, stability, and function. J Leukoc Biol. Jan 22–2018.(Epub ahead of print). doi: 10.1002/JLB.2MIR0817-349R. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Kuai J and Zhang N: Upregulation of SIRT1 by Evodiamine activates PI3K/AKT pathway and blocks intervertebral disc degeneration. Mol Med Rep 26: 265, 2022.
APA
Kuai, J., & Zhang, N. (2022). Upregulation of SIRT1 by Evodiamine activates PI3K/AKT pathway and blocks intervertebral disc degeneration. Molecular Medicine Reports, 26, 265. https://doi.org/10.3892/mmr.2022.12781
MLA
Kuai, J., Zhang, N."Upregulation of SIRT1 by Evodiamine activates PI3K/AKT pathway and blocks intervertebral disc degeneration". Molecular Medicine Reports 26.2 (2022): 265.
Chicago
Kuai, J., Zhang, N."Upregulation of SIRT1 by Evodiamine activates PI3K/AKT pathway and blocks intervertebral disc degeneration". Molecular Medicine Reports 26, no. 2 (2022): 265. https://doi.org/10.3892/mmr.2022.12781
Copy and paste a formatted citation
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Spandidos Publications style
Kuai J and Zhang N: Upregulation of SIRT1 by Evodiamine activates PI3K/AKT pathway and blocks intervertebral disc degeneration. Mol Med Rep 26: 265, 2022.
APA
Kuai, J., & Zhang, N. (2022). Upregulation of SIRT1 by Evodiamine activates PI3K/AKT pathway and blocks intervertebral disc degeneration. Molecular Medicine Reports, 26, 265. https://doi.org/10.3892/mmr.2022.12781
MLA
Kuai, J., Zhang, N."Upregulation of SIRT1 by Evodiamine activates PI3K/AKT pathway and blocks intervertebral disc degeneration". Molecular Medicine Reports 26.2 (2022): 265.
Chicago
Kuai, J., Zhang, N."Upregulation of SIRT1 by Evodiamine activates PI3K/AKT pathway and blocks intervertebral disc degeneration". Molecular Medicine Reports 26, no. 2 (2022): 265. https://doi.org/10.3892/mmr.2022.12781
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