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MW‑9, a chalcones derivative bearing heterocyclic moieties, attenuates experimental autoimmune encephalomyelitis via suppressing pathogenic TH17 cells

  • Authors:
    • Bei Liu
    • Zewei Mao
    • Na Yin
    • Qianlan Gu
    • Yan Qi
    • Xiaosi Li
    • Haihao Yang
    • Zhao Wu
    • Nanting Zou
    • Sai Ying
    • Chunping Wan
  • View Affiliations / Copyright

    Affiliations: School of Clinical Medicine, School of Pharmacy and School of Basic Medicine, Yunnan University of Traditional Chinese Medicine, Kunming, Yunnan 650021, P.R. China
    Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 308
    |
    Published online on: August 11, 2022
       https://doi.org/10.3892/mmr.2022.12824
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Abstract

Previous studies have indicated that MW‑9, a chalcones derivative bearing heterocyclic moieties, has considerable anti‑inflammatory activity in vitro. Whether MW‑9 may be used to treat inflammation‑based diseases, such as multiple sclerosis, remains unknown. The present study was designed to determine the effect and underlying mechanism of MW‑9 in experimental autoimmune encephalomyelitis (EAE). Female C57BL/6 mice immunized with MOG35‑55 were treated with or without MW‑9, then the clinical scores and other relevant parameters were investigated. Production of cytokines and specific antibodies were monitored by ELISA assays. Surface marker, Treg cell, and intracellular cytokines (IL‑17A and IFN‑γ) were detected by flow cytometry, and mRNA expression in the helper‑T (TH)17 cell‑related signaling pathway was examined by reverse transcription‑quantitative (RT‑q) PCR analysis. TH17 cell differentiation assay was performed. Herein, the present results demonstrated that oral administration of MW‑9 reduced the severity of disease in EAE mice through slowing down infiltration process, inhibiting the demyelination, blocking anti‑MOG35‑55 IgG antibody production (IgG, IgG2a and IgG3), and decreasing accumulation of CD11b+Gr‑1+ neutrophils from EAE mice. MW‑9 treatments also led to significantly decreased IL‑17A production and IL‑17 expression in CD4+ T‑cells, but had no detectable influence on development of TH1 and T‑regulatory cells ex vivo. RT‑qPCR analysis showed that within the spinal cords of the mice, MW‑9 blocked transcriptional expression of TH17‑associated genes, including Il17a, Il17f, Il6 and Ccr6. In TH17 cell differentiation assay, MW‑9 inhibited differentiation of ‘naïve’ CD4+ T‑cells into TH17 cells and reduced the IL‑17A production. The data demonstrated that MW‑9 could attenuate EAE in part through suppressing the formation and activities of pathogenic TH17 cells.
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1 

Liu Q, Gao Q, Zhang Y, Li Z and Mei X: MicroRNA-590 promotes pathogenic Th17 cell differentiation through targeting Tob1 and is associated with multiple sclerosis. Biochem Biophys Res Commun. 493:901–908. 2017. View Article : Google Scholar : PubMed/NCBI

2 

Handel AE, Lincoln MR and Ramagopalan SV: Of mice and men: Experimental autoimmune encephalitis and multiple sclerosis. Eur J Clin Invest. 41:1254–1258. 2011. View Article : Google Scholar : PubMed/NCBI

3 

Gran B, Zhang GX, Yu S, Li J, Chen XH, Ventura ES, Kamoun M and Rostami A: IL-12p35-deficient mice are susceptible to experimental autoimmune encephalomyelitis: Evidence for redundancy in the IL-12 system in the induction of central nervous system autoimmune demyelination. J Immunol. 169:7104–7110. 2002. View Article : Google Scholar : PubMed/NCBI

4 

El-behi M, Rostami A and Ciric B: Current views on the roles of Th1 and Th17 cells in experimental autoimmune encephalomyelitis. J Neuroimmune Pharmacol. 5:189–197. 2010. View Article : Google Scholar : PubMed/NCBI

5 

Cua DJ, Sherlock J, Chen Y, Murphy CA, Joyce B, Seymour B, Lucian L, To W, Kwan S, Churakova T, et al: Interleukin-23 rather than interleukin-12 is the critical cytokine for autoimmune inflammation of the brain. Nature. 421:744–748. 2003. View Article : Google Scholar : PubMed/NCBI

6 

Langrish CL, McKenzie BS, Wilson NJ, de Waal Malefyt R, Kastelein RA and Cua DJ: IL-12 and IL-23: Master regulators of innate and adaptive immunity. Immunol Rev. 202:96–105. 2004. View Article : Google Scholar : PubMed/NCBI

7 

Komiyama Y, Nakae S, Matsuki T, Nambu A, Ishigame H, Kakuta S, Sudo K and Iwakura Y: IL-17 plays an important role in the development of experimental autoimmune encephalomyelitis. J Immunol. 177:566–573. 2006. View Article : Google Scholar : PubMed/NCBI

8 

Lin Y, Hu C, Zheng X, Wang X and Mao Z: Synthesis and anti-tumor activities of novel 4′-(N-substitued-1-piperazinyl) chalcone derivatives. Chin J Org Chem. 37:237–241. 2017. View Article : Google Scholar

9 

Hui G, Xi Z, Ping Z, Si W, Chunping W, Gaoxionga R and Zewei M: Synthesis and biological evaluation of novel substituted chalcone-piperazine derivatives. Chin J Org Chem. 38:684–691. 2018. View Article : Google Scholar

10 

Mao Z, Zheng X, Lin Y, Qi Y, Hu C, Wan C and Rao G: Concise synthesis and biological evaluation of chalcone derivatives bearing N-heterocyclic moieties. Heterocycles. 92:1102–1110. 2016. View Article : Google Scholar

11 

Li X, Li TT, Zhang XH, Hou LF, Yang XQ, Zhu FH, Tang W and Zuo JP: Artemisinin analogue SM934 ameliorates murine experimental autoimmune encephalomyelitis through enhancing the expansion and functions of regulatory T cell. PLoS One. 8:e741082013. View Article : Google Scholar : PubMed/NCBI

12 

Contarini G, Giusti P and Skaper SD: Active induction of experimental autoimmune encephalomyelitis in C57BL/6 mice. Neurotrophic Factors. Springer; pp. 353–360. 2018, View Article : Google Scholar

13 

Hou L, Rao DA, Yuki K, Cooley J, Henderson LA, Jonsson AH, Kaiserman D, Gorman MP, Nigrovic PA, Bird PI, et al: SerpinB1 controls encephalitogenic T helper cells in neuroinflammation. Proc Natl Acad Sci USA. 116:20635–20643. 2019. View Article : Google Scholar : PubMed/NCBI

14 

Rho TW, Lee SY, Han SY, Kim JH, Lee KH, Kim DS, Kwak HB and Kim YK: Glycyrrhizae radix inhibits osteoclast differentiation by inhibiting c-Fos-dependent NFATc1 expression. Am J Chin Med. 45:283–298. 2017. View Article : Google Scholar : PubMed/NCBI

15 

Xu S, Zuo A, Guo Z and Wan C: Ethyl caffeate ameliorates collagen-induced arthritis by suppressing TH1 immune response. J Immunol Res. 2017. 74167922017.PubMed/NCBI

16 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

17 

Cao YJ, Xu Y, Liu B, Zheng X, Wu J, Zhang Y, Li XS, Qi Y, Sun YM, Wen WB, et al: Dioscin, a steroidal saponin isolated from dioscorea nipponica, attenuates collagen-induced arthritis by inhibiting Th17 cell response. Am J Chin Med. 47:423–437. 2019. View Article : Google Scholar : PubMed/NCBI

18 

Hou LF, He SJ, Li X, Yang Y, He PL, Zhou Y, Zhu FH, Yang YF, Li Y, Tang W and Zuo JP: Oral administration of artemisinin analog SM934 ameliorates lupus syndromes in MRL/lpr mice by inhibiting Th1 and Th17 cell responses. Arthritis Rheum. 63:2445–2455. 2011. View Article : Google Scholar : PubMed/NCBI

19 

Hou L, Cooley J, Swanson R, Ong PC, Pike RN, Bogyo M, Olson ST and Remold-O'Donnell E: The protease cathepsin L regulates Th17 cell differentiation. J Autoimmun. 65:56–63. 2015. View Article : Google Scholar : PubMed/NCBI

20 

Langrish CL, Chen Y, Blumenschein WM, Mattson J, Basham B, Sedgwick JD, McClanahan T, Kastelein RA and Cua DJ: IL-23 drives a pathogenic T cell population that induces autoimmune inflammation. J Exp Med. 201:233–240. 2005. View Article : Google Scholar : PubMed/NCBI

21 

Chung Y, Yang X, Chang SH, Ma L, Tian Q and Dong C: Expression and regulation of IL-22 in the IL-17-producing CD4+ T lymphocytes. Cell Res. 16:902–907. 2006. View Article : Google Scholar : PubMed/NCBI

22 

Brzustewicz E and Bryl E: The role of cytokines in the pathogenesis of rheumatoid arthritis-practical and potential application of cytokines as biomarkers and targets of personalized therapy. Cytokine. 76:527–536. 2015. View Article : Google Scholar : PubMed/NCBI

23 

Weaver CT, Harrington LE, Mangan PR, Gavrieli M and Murphy KM: Th17: An effector CD4 T cell lineage with regulatory T cell ties. Immunity. 24:677–688. 2006. View Article : Google Scholar : PubMed/NCBI

24 

Bettelli E, Oukka M and Kuchroo VK: T(H)-17 cells in the circle of immunity and autoimmunity. Nat Immunol. 8:345–350. 2007. View Article : Google Scholar : PubMed/NCBI

25 

Louten J, Boniface K and de Waal Malefyt R: Development and function of TH17 cells in health and disease. J Allergy Clin Immunol. 123:1004–1011. 2009. View Article : Google Scholar : PubMed/NCBI

26 

Zhou L, Ivanov II, Spolski R, Min R, Shenderov K, Egawa T, Levy DE, Leonard WJ and Littman DR: IL-6 programs T(H)-17 cell differentiation by promoting sequential engagement of the IL-21 and IL-23 pathways. Nat Immunol. 8:967–974. 2007. View Article : Google Scholar : PubMed/NCBI

27 

Zheng Y, Danilenko DM, Valdez P, Kasman I, Eastham-Anderson J, Wu J and Ouyang W: Interleukin-22, a T(H)17 cytokine, mediates IL-23-induced dermal inflammation and acanthosis. Nature. 445:648–651. 2007. View Article : Google Scholar : PubMed/NCBI

28 

Yang XO, Panopoulos AD, Nurieva R, Chang SH, Wang D, Watowich SS and Dong C: STAT3 regulates cytokine-mediated generation of inflammatory helper T cells. J Biol Chem. 282:9358–9363. 2007. View Article : Google Scholar : PubMed/NCBI

29 

Ivanov II, McKenzie BS, Zhou L, Tadokoro CE, Lepelley A, Lafaille JJ, Cua DJ and Littman DR: The orphan nuclear receptor RORgammat directs the differentiation program of proinflammatory IL-17+ T helper cells. Cell. 126:1121–1133. 2006. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Liu B, Mao Z, Yin N, Gu Q, Qi Y, Li X, Yang H, Wu Z, Zou N, Ying S, Ying S, et al: MW‑9, a chalcones derivative bearing heterocyclic moieties, attenuates experimental autoimmune encephalomyelitis via suppressing pathogenic T<sub>H</sub>17 cells. Mol Med Rep 26: 308, 2022.
APA
Liu, B., Mao, Z., Yin, N., Gu, Q., Qi, Y., Li, X. ... Wan, C. (2022). MW‑9, a chalcones derivative bearing heterocyclic moieties, attenuates experimental autoimmune encephalomyelitis via suppressing pathogenic T<sub>H</sub>17 cells. Molecular Medicine Reports, 26, 308. https://doi.org/10.3892/mmr.2022.12824
MLA
Liu, B., Mao, Z., Yin, N., Gu, Q., Qi, Y., Li, X., Yang, H., Wu, Z., Zou, N., Ying, S., Wan, C."MW‑9, a chalcones derivative bearing heterocyclic moieties, attenuates experimental autoimmune encephalomyelitis via suppressing pathogenic T<sub>H</sub>17 cells". Molecular Medicine Reports 26.4 (2022): 308.
Chicago
Liu, B., Mao, Z., Yin, N., Gu, Q., Qi, Y., Li, X., Yang, H., Wu, Z., Zou, N., Ying, S., Wan, C."MW‑9, a chalcones derivative bearing heterocyclic moieties, attenuates experimental autoimmune encephalomyelitis via suppressing pathogenic T<sub>H</sub>17 cells". Molecular Medicine Reports 26, no. 4 (2022): 308. https://doi.org/10.3892/mmr.2022.12824
Copy and paste a formatted citation
x
Spandidos Publications style
Liu B, Mao Z, Yin N, Gu Q, Qi Y, Li X, Yang H, Wu Z, Zou N, Ying S, Ying S, et al: MW‑9, a chalcones derivative bearing heterocyclic moieties, attenuates experimental autoimmune encephalomyelitis via suppressing pathogenic T<sub>H</sub>17 cells. Mol Med Rep 26: 308, 2022.
APA
Liu, B., Mao, Z., Yin, N., Gu, Q., Qi, Y., Li, X. ... Wan, C. (2022). MW‑9, a chalcones derivative bearing heterocyclic moieties, attenuates experimental autoimmune encephalomyelitis via suppressing pathogenic T<sub>H</sub>17 cells. Molecular Medicine Reports, 26, 308. https://doi.org/10.3892/mmr.2022.12824
MLA
Liu, B., Mao, Z., Yin, N., Gu, Q., Qi, Y., Li, X., Yang, H., Wu, Z., Zou, N., Ying, S., Wan, C."MW‑9, a chalcones derivative bearing heterocyclic moieties, attenuates experimental autoimmune encephalomyelitis via suppressing pathogenic T<sub>H</sub>17 cells". Molecular Medicine Reports 26.4 (2022): 308.
Chicago
Liu, B., Mao, Z., Yin, N., Gu, Q., Qi, Y., Li, X., Yang, H., Wu, Z., Zou, N., Ying, S., Wan, C."MW‑9, a chalcones derivative bearing heterocyclic moieties, attenuates experimental autoimmune encephalomyelitis via suppressing pathogenic T<sub>H</sub>17 cells". Molecular Medicine Reports 26, no. 4 (2022): 308. https://doi.org/10.3892/mmr.2022.12824
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