SAC3D1 activates Wnt/β‑catenin signalling in hepatocellular carcinoma

  • Authors:
    • Haitao Wang
    • Xiufang Shi
  • View Affiliations

  • Published online on: August 24, 2022     https://doi.org/10.3892/mmr.2022.12833
  • Article Number: 317
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Abstract

β‑catenin accumulates in hepatocellular carcinoma (HCC); therefore, understanding the mechanism of Wnt/β‑catenin pathway activation is important for HCC therapy. SAC3 domain containing 1 (SAC3D1) is involved in numerous types of cancer, such as gastric cancer. To the best of our knowledge, however, the role of SAC3D1 in HCC has not yet been elucidated. Here, the expression of SAC3D in HCC was examined by quantitative PCR, western blotting and immunohistochemistry. The function of SAC3D1 in HCC were examined using Cell Counting Kit‑8 and anchorage‑independent growth assay. It was found that the levels of SAC3D1 mRNA and protein were upregulated in HCC. When SAC3D1 was overexpressed, the proliferation of HCC cells was promoted; when the expression of SAC3D1 was disrupted, HCC cell growth was inhibited. When the molecular mechanism was investigated using immunoprecipitation, it was found that SAC3D1 interacted with axin, inhibiting ubiquitination of β‑catenin and elevating protein levels of β‑catenin. In summary, the present study revealed the promoting function of SAC3D1 in the progression of HCC. SAC3D1 may be a promising target for HCC therapy.
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October-2022
Volume 26 Issue 4

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Wang H and Wang H: SAC3D1 activates Wnt/β‑catenin signalling in hepatocellular carcinoma. Mol Med Rep 26: 317, 2022
APA
Wang, H., & Wang, H. (2022). SAC3D1 activates Wnt/β‑catenin signalling in hepatocellular carcinoma. Molecular Medicine Reports, 26, 317. https://doi.org/10.3892/mmr.2022.12833
MLA
Wang, H., Shi, X."SAC3D1 activates Wnt/β‑catenin signalling in hepatocellular carcinoma". Molecular Medicine Reports 26.4 (2022): 317.
Chicago
Wang, H., Shi, X."SAC3D1 activates Wnt/β‑catenin signalling in hepatocellular carcinoma". Molecular Medicine Reports 26, no. 4 (2022): 317. https://doi.org/10.3892/mmr.2022.12833