Palmitoyl‑RGD promotes the expression of dermal‑epidermal junction components in HaCaT cells

  • Authors:
    • Joo Hyuck Lim
    • Jung Soo Bae
    • Seung Ki Lee
    • Dong Hun Lee
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  • Published online on: August 29, 2022     https://doi.org/10.3892/mmr.2022.12836
  • Article Number: 320
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Abstract

With age, the dermal‑epidermal junction (DEJ) becomes thinner and production of its protein components decreases; this may be associated with increased fragility and wrinkling of skin. Topical treatment with palmitoyl‑Arg‑Gly‑Asp (PAL‑RGD) improves facial wrinkles, skin elasticity and dermal density in humans. In the present study, the effect of PAL‑RGD on expression of DEJ components, such as laminin and collagen, was assessed. Human HaCaT keratinocytes were treated with PAL‑RGD. The protein expression levels of laminin‑332, collagen IV and collagen XVII were examined by western blotting. Reverse transcription-quantitative PCR was used to analyze laminin subunit (LAM)A3, LAMB3, LAMC2, collagen type IV α 1 chain (COL4A1) and COL17A1 mRNA expression levels. Western blot analysis showed that the expression levels of proteins comprising the DEJ, including laminin α3, β3 and γ2 and collagen IV and XVII demonstrated a significant dose‑dependent increase following PAL‑RGD treatment. Furthermore, PAL‑RGD treatment significantly enhanced LAMA3, LAMB3, LAMC2, COL4A1 and COL17A1 mRNA expression levels. PAL‑RGD may enhance the DEJ by inducing the expression of laminin‑332, collagen IV and collagen XVII.
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October-2022
Volume 26 Issue 4

Print ISSN: 1791-2997
Online ISSN:1791-3004

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Spandidos Publications style
Lim JH, Bae JS, Lee SK and Lee DH: Palmitoyl‑RGD promotes the expression of dermal‑epidermal junction components in HaCaT cells. Mol Med Rep 26: 320, 2022
APA
Lim, J.H., Bae, J.S., Lee, S.K., & Lee, D.H. (2022). Palmitoyl‑RGD promotes the expression of dermal‑epidermal junction components in HaCaT cells. Molecular Medicine Reports, 26, 320. https://doi.org/10.3892/mmr.2022.12836
MLA
Lim, J. H., Bae, J. S., Lee, S. K., Lee, D. H."Palmitoyl‑RGD promotes the expression of dermal‑epidermal junction components in HaCaT cells". Molecular Medicine Reports 26.4 (2022): 320.
Chicago
Lim, J. H., Bae, J. S., Lee, S. K., Lee, D. H."Palmitoyl‑RGD promotes the expression of dermal‑epidermal junction components in HaCaT cells". Molecular Medicine Reports 26, no. 4 (2022): 320. https://doi.org/10.3892/mmr.2022.12836