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Article Open Access

ANRIL overexpression globally induces expression and alternative splicing of genes involved in inflammation in HUVECs

  • Authors:
    • Alimu Wufuer
    • Xiemusiye Luohemanjiang
    • Lei Du
    • Jing Lei
    • Mayila Shabier
    • Deng Feng Han
    • Jianhua Ma
  • View Affiliations / Copyright

    Affiliations: Department of Neurology, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang 830054, P.R. China
    Copyright: © Wufuer et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 27
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    Published online on: December 13, 2022
       https://doi.org/10.3892/mmr.2022.12915
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Abstract

Long non‑coding (lnc)RNAs serve important cellular functions and certain lncRNAs have roles in different mechanisms of gene regulation. lncRNA‑antisense non‑coding RNA in the INK4 locus (ANRIL) affects cell inflammation; however, the potential genes underlying the inflammatory response regulated by ANRIL remain unclear. In the present study, the potential function of ANRIL in regulating gene expression and alternative splicing was assessed. ANRIL‑regulated human umbilical vein endothelial cell (HUVEC) transcriptome was obtained using high‑throughput RNA sequencing (RNA‑seq) to evaluate the potential role of ANRIL. Following plasmid transfection, gene expression profile and alternative splicing pattern of HUVECs overexpressing ANRIL were analyzed using RNA‑seq. ANRIL overexpression affected the transcription levels of genes associated with the inflammatory response, NF‑κB signaling pathway, type I interferon‑mediated signal transduction pathway and innate immune response. ANRIL regulated the alternative splicing of hundreds of genes with functions such as gene expression, translation, DNA repair, RNA processing and participation in the NF‑κB signaling pathway. Many of these genes serve a key role in the inflammatory response. ANRIL‑regulated inflammatory response may be achieved by regulating alternate splicing and transcription. The present study broadened the understanding of ANRIL‑mediated gene regulation mechanisms and clarified the role of ANRIL in mediating inflammatory response mechanisms.
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Copy and paste a formatted citation
Spandidos Publications style
Wufuer A, Luohemanjiang X, Du L, Lei J, Shabier M, Han DF and Ma J: ANRIL overexpression globally induces expression and alternative splicing of genes involved in inflammation in HUVECs. Mol Med Rep 27: 27, 2023.
APA
Wufuer, A., Luohemanjiang, X., Du, L., Lei, J., Shabier, M., Han, D.F., & Ma, J. (2023). ANRIL overexpression globally induces expression and alternative splicing of genes involved in inflammation in HUVECs. Molecular Medicine Reports, 27, 27. https://doi.org/10.3892/mmr.2022.12915
MLA
Wufuer, A., Luohemanjiang, X., Du, L., Lei, J., Shabier, M., Han, D. F., Ma, J."ANRIL overexpression globally induces expression and alternative splicing of genes involved in inflammation in HUVECs". Molecular Medicine Reports 27.2 (2023): 27.
Chicago
Wufuer, A., Luohemanjiang, X., Du, L., Lei, J., Shabier, M., Han, D. F., Ma, J."ANRIL overexpression globally induces expression and alternative splicing of genes involved in inflammation in HUVECs". Molecular Medicine Reports 27, no. 2 (2023): 27. https://doi.org/10.3892/mmr.2022.12915
Copy and paste a formatted citation
x
Spandidos Publications style
Wufuer A, Luohemanjiang X, Du L, Lei J, Shabier M, Han DF and Ma J: ANRIL overexpression globally induces expression and alternative splicing of genes involved in inflammation in HUVECs. Mol Med Rep 27: 27, 2023.
APA
Wufuer, A., Luohemanjiang, X., Du, L., Lei, J., Shabier, M., Han, D.F., & Ma, J. (2023). ANRIL overexpression globally induces expression and alternative splicing of genes involved in inflammation in HUVECs. Molecular Medicine Reports, 27, 27. https://doi.org/10.3892/mmr.2022.12915
MLA
Wufuer, A., Luohemanjiang, X., Du, L., Lei, J., Shabier, M., Han, D. F., Ma, J."ANRIL overexpression globally induces expression and alternative splicing of genes involved in inflammation in HUVECs". Molecular Medicine Reports 27.2 (2023): 27.
Chicago
Wufuer, A., Luohemanjiang, X., Du, L., Lei, J., Shabier, M., Han, D. F., Ma, J."ANRIL overexpression globally induces expression and alternative splicing of genes involved in inflammation in HUVECs". Molecular Medicine Reports 27, no. 2 (2023): 27. https://doi.org/10.3892/mmr.2022.12915
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