RCAS1 increases cell morphological changes in murine fibroblasts by reducing p38 phosphorylation

Nishinakagawa,Takuya; Hazekawa,Mai; Hosokawa,Masato; Ishibashi,Daisuke

doi:10.3892/mmr.2023.12949

RCAS1 increases cell morphological changes in murine fibroblasts by reducing p38 phosphorylation

Authors:
- Takuya Nishinakagawa
- Mai Hazekawa
- Masato Hosokawa
- Daisuke Ishibashi
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Affiliations: Department of Immunological and Molecular Pharmacology, Faculty of Pharmaceutical Science, Fukuoka University, Fukuoka 814‑0180, Japan
Published online on: February 2, 2023 https://doi.org/10.3892/mmr.2023.12949
Article Number: 62
Copyright: © Nishinakagawa et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Receptor‑binding cancer antigen expressed on SiSo cells (RCAS1) is a tumor‑associated antigen that is expressed in a number of human malignancies. RCAS1 acts as a ligand for a putative RCAS1 receptor that is present on various human cells including T and B lymphocytes and natural killer cells, in which it induces cell growth inhibition and apoptosis. It has been suggested that RCAS1 might serve an important role in tumor cell evasion from the host immune system. In fact, RCAS1 expression is related to malignant characteristics including tumor size, invasion depth, clinical stage and poor overall survival. The authors previously established doxycycline‑induced RCAS1 overexpression murine fibroblast L cells to analyze the biological functions of RCAS1 and reported that its expression inhibited cell cycle progression via the downregulation of cyclin D3, which subsequently induced apoptosis. Additionally, it was found that RCAS1 expression induced cell morphological changes prior to caspase‑mediated apoptosis. Thus, the present study examined signaling pathways associated with changes in cell morphology that were induced by RCAS1 expression. The data showed that increased RCAS1 expression caused a reduction in actin stress fibers and decreased cofilin phosphorylation. Recent studies have shown that p38 signaling regulates actin polymerization. The data the present study showed that increased RCAS1 expression significantly decreased p38 phosphorylation.

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