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Article Special Issue Open Access

Oviduct epithelial cell‑derived extracellular vesicles promote the developmental competence of IVF porcine embryos

This article is part of the special Issue: Molecular mammalian reproduction
  • Authors:
    • Xun Fang
    • Seonggyu Bang
    • Bereket Molla Tanga
    • Chaerim Seo
    • Dongjie Zhou
    • Gyeonghwan Seong
    • Islam M. Saadeldin
    • Sanghoon Lee
    • Xiang-Shun Cui
    • Jongki Cho
  • View Affiliations / Copyright

    Affiliations: Laboratory of Theriogenology, College of Veterinary Medicine, Chungnam National University, Daejeon 34134, Republic of Korea, Department of Animal Sciences, Chungbuk National University, Cheongju 28644, Republic of Korea
    Copyright: © Fang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 122
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    Published online on: May 12, 2023
       https://doi.org/10.3892/mmr.2023.13009
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Abstract

Assisted reproductive technology has increased the efficiency of animal reproduction. However, polyspermy is a significant limitation of porcine in vitro fertilization (IVF). Therefore, reducing the polyspermy rate and improving monospermic embryos is crucial. Recent studies have reported that oviductal fluid, along with its contents of extracellular vesicles (EVs), enhanced the fertilization process and supported embryo development. Consequently, the present study investigated the effects of porcine oviduct epithelial cells (OEC‑EVs) on sperm‑oocyte interactions during porcine IVF and evaluated in vitro embryo developmental competence outcomes. During IVF embryo development, the cleavage rate was significantly higher in the group treated with 50 ng/ml OEC‑EVs compared with the control group (67.6±2.5 vs. 57.3±1.9; P<0.05). Furthermore, the OEC‑EV group had significantly more embryos (16.4±1.2 vs. 10.2±0.8; P<0.05), and the polyspermy rate significantly decreased (32.9±2.5 vs. 43.8±3.1; P<0.05) compared with that of the control group. Additionally, the fluorescence intensities of cortical granules (3.56±0.47 vs. 2.15±0.24; P<0.05) and active mitochondria (8.14±0.34 vs. 5.96±0.38; P<0.05) were significantly higher in the OEC‑EV group compared with those in the control group. In conclusion, OEC‑EV adsorption and penetration crosstalk between sperm and oocytes was observed. OEC‑EV treatment was demonstrated to significantly improve the concentration and distribution of cortical granules in oocytes. Furthermore, OEC‑EVs also increased oocyte mitochondrial activity, reduced polyspermy and increased the IVF success rate.
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Copy and paste a formatted citation
Spandidos Publications style
Fang X, Bang S, Tanga BM, Seo C, Zhou D, Seong G, Saadeldin IM, Lee S, Cui X, Cho J, Cho J, et al: Oviduct epithelial cell‑derived extracellular vesicles promote the developmental competence of IVF porcine embryos. Mol Med Rep 27: 122, 2023.
APA
Fang, X., Bang, S., Tanga, B.M., Seo, C., Zhou, D., Seong, G. ... Cho, J. (2023). Oviduct epithelial cell‑derived extracellular vesicles promote the developmental competence of IVF porcine embryos. Molecular Medicine Reports, 27, 122. https://doi.org/10.3892/mmr.2023.13009
MLA
Fang, X., Bang, S., Tanga, B. M., Seo, C., Zhou, D., Seong, G., Saadeldin, I. M., Lee, S., Cui, X., Cho, J."Oviduct epithelial cell‑derived extracellular vesicles promote the developmental competence of IVF porcine embryos". Molecular Medicine Reports 27.6 (2023): 122.
Chicago
Fang, X., Bang, S., Tanga, B. M., Seo, C., Zhou, D., Seong, G., Saadeldin, I. M., Lee, S., Cui, X., Cho, J."Oviduct epithelial cell‑derived extracellular vesicles promote the developmental competence of IVF porcine embryos". Molecular Medicine Reports 27, no. 6 (2023): 122. https://doi.org/10.3892/mmr.2023.13009
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x
Spandidos Publications style
Fang X, Bang S, Tanga BM, Seo C, Zhou D, Seong G, Saadeldin IM, Lee S, Cui X, Cho J, Cho J, et al: Oviduct epithelial cell‑derived extracellular vesicles promote the developmental competence of IVF porcine embryos. Mol Med Rep 27: 122, 2023.
APA
Fang, X., Bang, S., Tanga, B.M., Seo, C., Zhou, D., Seong, G. ... Cho, J. (2023). Oviduct epithelial cell‑derived extracellular vesicles promote the developmental competence of IVF porcine embryos. Molecular Medicine Reports, 27, 122. https://doi.org/10.3892/mmr.2023.13009
MLA
Fang, X., Bang, S., Tanga, B. M., Seo, C., Zhou, D., Seong, G., Saadeldin, I. M., Lee, S., Cui, X., Cho, J."Oviduct epithelial cell‑derived extracellular vesicles promote the developmental competence of IVF porcine embryos". Molecular Medicine Reports 27.6 (2023): 122.
Chicago
Fang, X., Bang, S., Tanga, B. M., Seo, C., Zhou, D., Seong, G., Saadeldin, I. M., Lee, S., Cui, X., Cho, J."Oviduct epithelial cell‑derived extracellular vesicles promote the developmental competence of IVF porcine embryos". Molecular Medicine Reports 27, no. 6 (2023): 122. https://doi.org/10.3892/mmr.2023.13009
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