hsa‑miR‑216a‑3p regulates cell proliferation in oral cancer via the Wnt3a/β‑catenin pathway

Wang,Yifan; Wang,Yifan; Liu,Shanshan; Liu,Shanshan; Lv,Feifei; Lv,Feifei; Zhai,Wenjing; Zhai,Wenjing; Wang,Weina; Wang,Weina; Duan,Yanhao; Duan,Yanhao; Qiu,Yongle; Qiu,Yongle

doi:10.3892/mmr.2023.13015

hsa‑miR‑216a‑3p regulates cell proliferation in oral cancer via the Wnt3a/β‑catenin pathway

Authors:
- Yifan Wang
- Shanshan Liu
- Feifei Lv
- Wenjing Zhai
- Weina Wang
- Yanhao Duan
- Yongle Qiu
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Affiliations: Department of Stomatology, Affiliated Hospital of Hebei University, Baoding, Hebei 071000, P.R. China, Department of Stomatology, The Fourth Hospital of Hebei Medical University, Shijiazhuang, Hebei 050017, P.R. China, Department of Stomatology, People's Hospital of Shijiazhuang, Shijiazhuang, Hebei 050000, P.R. China, Department of Cardiology, Zhangjiakou First Hospital, Zhangjiakou, Hebei 075000, P.R. China
Published online on: May 16, 2023 https://doi.org/10.3892/mmr.2023.13015
Article Number: 128
Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.

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Abstract

Oral cancer is one of the leading causes of death worldwide, with a reported 5‑year survival rate of ~50% after treatment. The treatment measures for oral cancer are very expensive and affordability is low. Thus, it is necessary to develop more effective therapies to treat oral cancer. A number of studies have found that miRNAs are invasive biomarkers and have therapeutic potential in a variety of cancers. The present study included 30 oral patients and 30 healthy controls. Clinicopathological characteristic and miR‑216a‑3p/β‑catenin expression level of 30 oral cancer patients were analyzed. In addition, two oral cancer cell lines (HSC‑6 and CAL‑27) were used for mechanism‑of‑action study. The expression level of miR‑216a‑3p was higher in oral cancer patients compared with healthy controls and positively associated with tumor stage. Inhibition of miR‑216a‑3p potently suppressed cell viability and induced apoptosis of oral cancer cells. It was found that effects of miR‑216a‑3p on oral cancer were through Wnt3a signaling. It was also found that the expression level of β‑catenin was higher in oral cancer patients compared with healthy controls and positively associated with tumor stage; the effects of miR‑216a‑3p on oral cancer were through β‑catenin. In conclusion, miR‑216a‑3p and the Wnt‑β‑catenin signaling pathway may be interesting candidates to develop effective therapies for oral cancers.

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