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Article Open Access

ORMDL3‑mediated bronchial epithelial pyroptosis leads to lung inflammation in obese mice with asthma

  • Authors:
    • Fan Liu
    • Yan Sun
    • Yun Zhou
    • Yuye Gao
    • Qijun Song
    • Jianmei Yang
    • Chao Xu
    • Guimei Li
  • View Affiliations / Copyright

    Affiliations: Children's Hospital Capital Institute of Pediatrics, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100020, P.R. China, Department of Pediatrics, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong 250021, P.R. China, Department of Pediatrics, Shandong Provincial Lanling People's Hospital, Linyi, Shandong 277799, P.R. China, Department of Endocrinology and Metabolism, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong 250021, P.R. China
    Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 186
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    Published online on: August 17, 2023
       https://doi.org/10.3892/mmr.2023.13073
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Abstract

Asthma associated with obesity is a chronic disease that poses a threat to health in children and results in severe wheezing, earlier airway remodeling and increased insensitivity to hormone therapy compared with those who only have asthma. Despite its clinical importance, knowledge on the underlying mechanisms of this disease is limited. The present study aimed to elucidate the pathogenesis of asthma associated with obesity using a murine model. A total of 30 female BALB/c mice were divided into three groups: Normal, mice with asthma and obese mice with asthma. Obese mice with asthma were fed a high‑fat diet to induce obesity. Mice with asthma were sensitized and challenged with ovalbumin (OVA). Obese mice were subjected to OVA sensitization and challenge to develop asthma associated with obesity. Airway remodeling was observed in obese mice with asthma through HE and Masson staining. Proteomic and bioinformatics analyses were conducted on lung tissue from obese mice with asthma and normal mice. A total of 200 proteins were differentially expressed in obese mice with asthma compared with normal mice; of these, 53 and 47% were up‑ and downregulated, respectively. Pathway analysis revealed that asthma associated with obesity primarily affected the ‘lysosome’, ‘phagosome’, and ‘sphingolipid metabolism’ pathways. Gene Set Enrichment Analysis demonstrated the presence of pyroptosis in obese asthmatic mice, along with significant increases in pyroptosis‑­associated factors such as GSDMD and Caspase. High protein expression of orosomucoid‑like 3 (ORMDL3), NOD‑like receptor thermal protein domain associated protein 3 (NLRP3) and Gasdermin‑D (GSDMD) was observed in obese mice with asthma. In vitro experiments using HBE cells infected with ORMDL3‑overexpressing lentivirus demonstrated that the overexpression of ORMDL3 led to increased expression of NLRP3, GSDMD and cathepsin D (CTSD). These findings suggested that ORMDL3 may regulate pyroptosis and subsequent airway remodeling in asthma associated with obesity via the CTSD/NLRP3/GSDMD pathway.
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Copy and paste a formatted citation
Spandidos Publications style
Liu F, Sun Y, Zhou Y, Gao Y, Song Q, Yang J, Xu C and Li G: ORMDL3‑mediated bronchial epithelial pyroptosis leads to lung inflammation in obese mice with asthma. Mol Med Rep 28: 186, 2023.
APA
Liu, F., Sun, Y., Zhou, Y., Gao, Y., Song, Q., Yang, J. ... Li, G. (2023). ORMDL3‑mediated bronchial epithelial pyroptosis leads to lung inflammation in obese mice with asthma. Molecular Medicine Reports, 28, 186. https://doi.org/10.3892/mmr.2023.13073
MLA
Liu, F., Sun, Y., Zhou, Y., Gao, Y., Song, Q., Yang, J., Xu, C., Li, G."ORMDL3‑mediated bronchial epithelial pyroptosis leads to lung inflammation in obese mice with asthma". Molecular Medicine Reports 28.4 (2023): 186.
Chicago
Liu, F., Sun, Y., Zhou, Y., Gao, Y., Song, Q., Yang, J., Xu, C., Li, G."ORMDL3‑mediated bronchial epithelial pyroptosis leads to lung inflammation in obese mice with asthma". Molecular Medicine Reports 28, no. 4 (2023): 186. https://doi.org/10.3892/mmr.2023.13073
Copy and paste a formatted citation
x
Spandidos Publications style
Liu F, Sun Y, Zhou Y, Gao Y, Song Q, Yang J, Xu C and Li G: ORMDL3‑mediated bronchial epithelial pyroptosis leads to lung inflammation in obese mice with asthma. Mol Med Rep 28: 186, 2023.
APA
Liu, F., Sun, Y., Zhou, Y., Gao, Y., Song, Q., Yang, J. ... Li, G. (2023). ORMDL3‑mediated bronchial epithelial pyroptosis leads to lung inflammation in obese mice with asthma. Molecular Medicine Reports, 28, 186. https://doi.org/10.3892/mmr.2023.13073
MLA
Liu, F., Sun, Y., Zhou, Y., Gao, Y., Song, Q., Yang, J., Xu, C., Li, G."ORMDL3‑mediated bronchial epithelial pyroptosis leads to lung inflammation in obese mice with asthma". Molecular Medicine Reports 28.4 (2023): 186.
Chicago
Liu, F., Sun, Y., Zhou, Y., Gao, Y., Song, Q., Yang, J., Xu, C., Li, G."ORMDL3‑mediated bronchial epithelial pyroptosis leads to lung inflammation in obese mice with asthma". Molecular Medicine Reports 28, no. 4 (2023): 186. https://doi.org/10.3892/mmr.2023.13073
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