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Article Open Access

An intricate regulatory circuit between FLI1 and GATA1/GATA2/LDB1/ERG dictates erythroid vs. megakaryocytic differentiation

  • Authors:
    • Chunlin Wang
    • Maoting Hu
    • Kunlin Yu
    • Wuling Liu
    • Anling Hu
    • Yi Kuang
    • Lei Huang
    • Babu Gajendran
    • Eldad Zacksenhaus
    • Xiao Xiao
    • Yaacov Ben‑David
  • View Affiliations / Copyright

    Affiliations: State Key Laboratory for Functions and Applications of Medicinal Plants, Guizhou Medical University, Guiyang, Guizhou 550014, P.R. China, Department of Medicine, University of Toronto, Toronto, Ontario M5S3H2, Canada
    Copyright: © Wang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 107
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    Published online on: April 26, 2024
       https://doi.org/10.3892/mmr.2024.13231
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Abstract

During hematopoiesis, megakaryocytic erythroid progenitors (MEPs) differentiate into megakaryocytic or erythroid lineages in response to specific transcriptional factors, yet the regulatory mechanism remains to be elucidated. Using the MEP‑like cell line HEL western blotting, RT‑qPCR, lentivirus‑mediated downregulation, flow cytometry as well as chromatin immunoprecipitation (ChIp) assay demonstrated that the E26 transformation‑specific (ETS) transcription factor friend leukemia integration factor 1 (Fli‑1) inhibits erythroid differentiation. The present study using these methods showed that while FLI1‑mediated downregulation of GATA binding protein 1 (GATA1) suppresses erythropoiesis, its direct transcriptional induction of GATA2 promotes megakaryocytic differentiation. GATA1 is also involved in megakaryocytic differentiation through regulation of GATA2. By contrast to FLI1, the ETS member erythroblast transformation‑specific‑related gene (ERG) negatively controls GATA2 and its overexpression through exogenous transfection blocks megakaryocytic differentiation. In addition, FLI1 regulates expression of LIM Domain Binding 1 (LDB1) during erythroid and megakaryocytic commitment, whereas shRNA‑mediated depletion of LDB1 downregulates FLI1 and GATA2 but increases GATA1 expression. In agreement, LDB1 ablation using shRNA lentivirus expression blocks megakaryocytic differentiation and modestly suppresses erythroid maturation. These results suggested that a certain threshold level of LDB1 expression enables FLI1 to block erythroid differentiation. Overall, FLI1 controlled the commitment of MEP to either erythroid or megakaryocytic lineage through an intricate regulation of GATA1/GATA2, LDB1 and ERG, exposing multiple targets for cell fate commitment and therapeutic intervention.
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1 

Orkin SH and Zon LI: Hematopoiesis: An evolving paradigm for stem cell biology. Cell. 132:631–644. 2008. View Article : Google Scholar : PubMed/NCBI

2 

Teitell MA and Mikkola HK: Transcriptional activators, repressors, and epigenetic modifiers controlling hematopoietic stem cell development. Pediatr Res. 59:33R–39R. 2006. View Article : Google Scholar : PubMed/NCBI

3 

Wickrema A and Crispino JD: Erythroid and megakaryocytic transformation. Oncogene. 26:6803–6815. 2007. View Article : Google Scholar : PubMed/NCBI

4 

Tijssen MR, Cvejic A, Joshi A, Hannah RL, Ferreira R, Forrai A, Bellissimo DC, Oram SH, Smethurst PA, Wilson NK, et al: Genome-wide analysis of simultaneous GATA1/2, RUNX1, FLI1, and SCL binding in megakaryocytes identifies hematopoietic regulators. Dev Cell. 20:597–609. 2011. View Article : Google Scholar : PubMed/NCBI

5 

Subramanian S, Thoms JAI, Huang Y, Cornejo-Páramo P, Koch FC, Jacquelin S, Shen S, Song E, Joshi S, Brownlee C, et al: Genome-wide transcription factor binding maps reveal cell-specific changes in the regulatory architecture of human HSPC. Blood. 142:1448–1462. 2023. View Article : Google Scholar : PubMed/NCBI

6 

Beck D, Thoms JA, Perera D, Schütte J, Unnikrishnan A, Knezevic K, Kinston SJ, Wilson NK, O'Brien TA, Göttgens B, et al: Genome-wide analysis of transcriptional regulators in human HSPCs reveals a densely interconnected network of coding and noncoding genes. Blood. 122:e12–e22. 2013. View Article : Google Scholar : PubMed/NCBI

7 

Diffner E, Beck D, Gudgin E, Thoms JA, Knezevic K, Pridans C, Foster S, Goode D, Lim WK, Boelen L, et al: Activity of a heptad of transcription factors is associated with stem cell programs and clinical outcome in acute myeloid leukemia. Blood. 121:2289–2300. 2013. View Article : Google Scholar : PubMed/NCBI

8 

Ben-David Y, Giddens EB and Bernstein A: Identification and mapping of a common proviral integration site Fli-1 in erythroleukemia cells induced by friend murine leukemia virus. Proc Natl Acad Sci USA. 87:1332–1336. 1990. View Article : Google Scholar : PubMed/NCBI

9 

Ben-David Y, Giddens EB, Letwin K and Bernstein A: Erythroleukemia induction by Friend murine leukemia virus: Insertional activation of a new member of the ets gene family, Fli-1, closely linked to c-ets-1. Genes Dev. 5:908–918. 1991. View Article : Google Scholar : PubMed/NCBI

10 

Pereira R, Quang CT, Lesault I, Dolznig H, Beug H and Ghysdael J: FLI-1 inhibits differentiation and induces proliferation of primary erythroblasts. Oncogene. 18:1597–1608. 1999. View Article : Google Scholar : PubMed/NCBI

11 

Tamir A, Howard J, Higgins RR, Li YJ, Berger L, Zacksenhaus E, Reis M and Ben-David Y: Fli-1, an Ets-related transcription factor, regulates erythropoietin-induced erythroid proliferation and differentiation: Evidence for direct transcriptional repression of the Rb gene during differentiation. Mol Cell Biol. 19:4452–4464. 1999. View Article : Google Scholar : PubMed/NCBI

12 

Athanasiou M, Mavrothalassitis G, Sun-Hoffman L and Blair DG: FLI-1 is a suppressor of erythroid differentiation in human hematopoietic cells. Leukemia. 14:439–445. 2000. View Article : Google Scholar : PubMed/NCBI

13 

Starck J, Weiss-Gayet M, Gonnet C, Guyot B, Vicat JM and Morlé F: Inducible Fli-1 gene deletion in adult mice modifies several myeloid lineage commitment decisions and accelerates proliferation arrest and terminal erythrocytic differentiation. Blood. 116:4795–4805. 2010. View Article : Google Scholar : PubMed/NCBI

14 

Liu T, Yao Y, Zhang G, Wang Y, Deng B, Song J, Li X, Han F, Xiao X, Yang J, et al: A screen for Fli-1 transcriptional modulators identifies PKC agonists that induce erythroid to megakaryocytic differentiation and suppress leukemogenesis. Oncotarget. 8:16728–16743. 2017. View Article : Google Scholar : PubMed/NCBI

15 

Liu F, Walmsley M, Rodaway A and Patient R: Fli1 acts at the top of the transcriptional network driving blood and endothelial development. Curr Biol. 18:1234–1240. 2008. View Article : Google Scholar : PubMed/NCBI

16 

Ben-David Y, Gajendran B, Sample KM and Zacksenhaus E: Current insights into the role of Fli-1 in hematopoiesis and malignant transformation. Cell Mol Life Sci. 79:1632022. View Article : Google Scholar : PubMed/NCBI

17 

Love PE, Warzecha C and Li L: Ldb1 complexes: The new master regulators of erythroid gene transcription. Trends Genet. 30:1–9. 2014. View Article : Google Scholar : PubMed/NCBI

18 

Li L, Lee JY, Gross J, Song SH, Dean A and Love PE: A requirement for Lim domain binding protein 1 in erythropoiesis. J Exp Med. 207:2543–2550. 2010. View Article : Google Scholar : PubMed/NCBI

19 

Lee J, Krivega I, Dale RK and Dean A: The LDB1 complex Co-opts CTCF for erythroid lineage-specific long-range enhancer interactions. Cell Rep. 19:2490–2502. 2017. View Article : Google Scholar : PubMed/NCBI

20 

Soler E, Andrieu-Soler C, de Boer E, Bryne JC, Thongjuea S, Stadhouders R, Palstra RJ, Stevens M, Kockx C, van Ijcken W, et al: The genome-wide dynamics of the binding of Ldb1 complexes during erythroid differentiation. Genes Dev. 24:277–289. 2010. View Article : Google Scholar : PubMed/NCBI

21 

Krivega I, Dale RK and Dean A: Role of LDB1 in the transition from chromatin looping to transcription activation. Genes Dev. 28:1278–1290. 2014. View Article : Google Scholar : PubMed/NCBI

22 

Deng W, Lee J, Wang H, Miller J, Reik A, Gregory PD, Dean A and Blobel GA: Controlling long-range genomic interactions at a native locus by targeted tethering of a looping factor. Cell. 149:1233–1244. 2012. View Article : Google Scholar : PubMed/NCBI

23 

Stadhouders R, Cico A, Stephen T, Thongjuea S, Kolovos P, Baymaz HI, Yu X, Demmers J, Bezstarosti K, Maas A, et al: Control of developmentally primed erythroid genes by combinatorial co-repressor actions. Nat Commun. 6:88932015. View Article : Google Scholar : PubMed/NCBI

24 

Giraud G, Kolovos P, Boltsis I, van Staalduinen J, Guyot B, Weiss-Gayet M, IJcken WV, Morlé F and Grosveld F: Interplay between FLI-1 and the LDB1 complex in murine erythroleukemia cells and during megakaryopoiesis. iScience. 24:1022102021. View Article : Google Scholar : PubMed/NCBI

25 

Eisbacher M, Holmes ML, Newton A, Hogg PJ, Khachigian LM, Crossley M and Chong BH: Protein-protein interaction between Fli-1 and GATA-1 mediates synergistic expression of megakaryocyte-specific genes through cooperative DNA binding. Mol Cell Biol. 23:3427–3441. 2003. View Article : Google Scholar : PubMed/NCBI

26 

Li YJ, Zhao X, Vecchiarelli-Federico LM, Li Y, Datti A, Cheng Y and Ben-David Y: Drug-mediated inhibition of Fli-1 for the treatment of leukemia. Blood Cancer J. 2:e542012. View Article : Google Scholar : PubMed/NCBI

27 

Wang C, Song J, Liu W, Yao Y, Kapranov P, Sample KM, Gajendran B, Zacksenhaus E, Hao X and Ben-David Y: FLI1 promotes protein translation via the transcriptional regulation of MKNK1 expression. Int J Oncol. 56:430–438. 2020.PubMed/NCBI

28 

Livak KJ and Schmittgen TD: Analysis of relative gene expression data using real-time quantitative PCR and the 2(−Delta Delta C(T)) method. Methods. 25:402–408. 2001. View Article : Google Scholar : PubMed/NCBI

29 

Wang C, Sample KM, Gajendran B, Kapranov P, Liu W, Hu A, Zacksenhaus E, Li Y, Hao X and Ben-David Y: FLI1 induces megakaryopoiesis gene expression through WAS/WIP-dependent and independent mechanisms; Implications for wiskott-aldrich syndrome. Front Immunol. 12:6078362021. View Article : Google Scholar : PubMed/NCBI

30 

Chen C, Chen H, Zhang Y, Thomas HR, Frank MH, He Y and Xia R: TBtools: An integrative toolkit developed for interactive analyses of big biological data. Mol Plant. 13:1194–1202. 2020. View Article : Google Scholar : PubMed/NCBI

31 

Song J, Yuan C, Yang J, Liu T, Yao Y, Xiao X, Gajendran B, Xu D, Li YJ, Wang C, et al: Novel flavagline-like compounds with potent Fli-1 inhibitory activity suppress diverse types of leukemia. FEBS J. 285:4631–4645. 2018. View Article : Google Scholar : PubMed/NCBI

32 

Barbeau B, Barat C, Bergeron D and Rassart E: The GATA-1 and Spi-1 transcriptional factors bind to a GATA/EBS dual element in the Fli-1 exon 1. Oncogene. 18:5535–5545. 1999. View Article : Google Scholar : PubMed/NCBI

33 

An X and Chen L: Flow cytometry (FCM) analysis and fluorescence-activated cell sorting (FACS) of erythroid cells. Methods Mol Biol. 1698:153–174. 2018. View Article : Google Scholar : PubMed/NCBI

34 

Nakahata T and Okumura N: Cell surface antigen expression in human erythroid progenitors: Erythroid and megakaryocytic markers. Leuk Lymphoma. 13:401–409. 1994. View Article : Google Scholar : PubMed/NCBI

35 

ENCODE Project Consortium, . An integrated encyclopedia of DNA elements in the human genome. Nature. 489:57–74. 2012. View Article : Google Scholar : PubMed/NCBI

36 

Zhang P, Basu P, Redmond LC, Morris PE, Rupon JW, Ginder GD and Lloyd JA: A functional screen for Krüppel-like factors that regulate the human gamma-globin gene through the CACCC promoter element. Blood Cells Mol Dis. 35:227–235. 2005. View Article : Google Scholar : PubMed/NCBI

37 

Starck J, Cohet N, Gonnet C, Sarrazin S, Doubeikovskaia Z, Doubeikovski A, Verger A, Duterque-Coquillaud M and Morle F: Functional cross-antagonism between transcription factors FLI-1 and EKLF. Mol Cell Biol. 23:1390–1402. 2003. View Article : Google Scholar : PubMed/NCBI

38 

Neuwirtova R, Fuchs O, Holicka M, Vostry M, Kostecka A, Hajkova H, Jonasova A, Cermak J, Cmejla R, Pospisilova D, et al: Transcription factors Fli1 and EKLF in the differentiation of megakaryocytic and erythroid progenitor in 5q-syndrome and in Diamond-Blackfan anemia. Ann Hematol. 92:11–18. 2013. View Article : Google Scholar : PubMed/NCBI

39 

Merika M and Orkin SH: Functional synergy and physical interactions of the erythroid transcription factor GATA-1 with the Krüppel family proteins Sp1 and EKLF. Mol Cell Biol. 15:2437–2447. 1995. View Article : Google Scholar : PubMed/NCBI

40 

Yamaoka A, Suzuki M, Katayama S, Orihara D, Engel JD and Yamamoto M: EVI1 and GATA2 misexpression induced by inv(3)(q21q26) contribute to megakaryocyte-lineage skewing and leukemogenesis. Blood Adv. 4:1722–1736. 2020. View Article : Google Scholar : PubMed/NCBI

41 

Johnson KD, Conn DJ, Shishkova E, Katsumura KR, Liu P, Shen S, Ranheim EA, Kraus SG, Wang W, Calvo KR, et al: Constructing and deconstructing GATA2-regulated cell fate programs to establish developmental trajectories. J Exp Med. 217:e201915262020. View Article : Google Scholar : PubMed/NCBI

42 

Yi G, Mandoli A, Jussen L, Tijchon E, van Bergen MGJM, Cordonnier G, Hansen M, Kim B, Nguyen LN, Jansen PWTC, et al: CBFβ-MYH11 interferes with megakaryocyte differentiation via modulating a gene program that includes GATA2 and KLF1. Blood Cancer J. 9:332019. View Article : Google Scholar : PubMed/NCBI

43 

Ikonomi P, Rivera CE, Riordan M, Washington G, Schechter AN and Noguchi CT: Overexpression of GATA-2 inhibits erythroid and promotes megakaryocyte differentiation. Exp Hematol. 28:1423–1431. 2000. View Article : Google Scholar : PubMed/NCBI

44 

Li Y, Ke Q, Shao Y, Zhu G, Li Y, Geng N, Jin F and Li F: GATA1 induces epithelial-mesenchymal transition in breast cancer cells through PAK5 oncogenic signaling. Oncotarget. 6:4345–4356. 2015. View Article : Google Scholar : PubMed/NCBI

45 

Yu J, Liu M, Liu H and Zhou L: GATA1 promotes colorectal cancer cell proliferation, migration and invasion via activating AKT signaling pathway. Mol Cell Biochem. 457:191–199. 2019. View Article : Google Scholar : PubMed/NCBI

46 

Carmichael CL, Metcalf D, Henley KJ, Kruse EA, Di Rago L, Mifsud S, Alexander WS and Kile BT: Hematopoietic overexpression of the transcription factor Erg induces lymphoid and erythro-megakaryocytic leukemia. Proc Natl Acad Sci USA. 109:15437–15442. 2012. View Article : Google Scholar : PubMed/NCBI

47 

Mukhopadhyay M, Teufel A, Yamashita T, Agulnick AD, Chen L, Downs KM, Schindler A, Grinberg A, Huang SP, Dorward D and Westphal H: Functional ablation of the mouse Ldb1 gene results in severe patterning defects during gastrulation. Development. 130:495–505. 2003. View Article : Google Scholar : PubMed/NCBI

48 

Song SH, Kim A, Ragoczy T, Bender MA, Groudine M and Dean A: Multiple functions of Ldb1 required for beta-globin activation during erythroid differentiation. Blood. 116:2356–2364. 2010. View Article : Google Scholar : PubMed/NCBI

49 

Visvader JE, Mao X, Fujiwara Y, Hahm K and Orkin SH: The LIM-domain binding protein Ldb1 and its partner LMO2 act as negative regulators of erythroid differentiation. Proc Natl Acad Sci USA. 94:13707–13712. 1997. View Article : Google Scholar : PubMed/NCBI

50 

Liu T, Xia L, Yao Y, Yan C, Fan Y, Gajendran B, Yang J, Li YJ, Chen J, Filmus J, et al: Identification of diterpenoid compounds that interfere with Fli-1 DNA binding to suppress leukemogenesis. Cell Death Dis. 10:1172019. View Article : Google Scholar : PubMed/NCBI

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Copy and paste a formatted citation
Spandidos Publications style
Wang C, Hu M, Yu K, Liu W, Hu A, Kuang Y, Huang L, Gajendran B, Zacksenhaus E, Xiao X, Xiao X, et al: An intricate regulatory circuit between FLI1 and GATA1/GATA2/LDB1/ERG dictates erythroid vs. megakaryocytic differentiation. Mol Med Rep 29: 107, 2024.
APA
Wang, C., Hu, M., Yu, K., Liu, W., Hu, A., Kuang, Y. ... Ben‑David, Y. (2024). An intricate regulatory circuit between FLI1 and GATA1/GATA2/LDB1/ERG dictates erythroid vs. megakaryocytic differentiation. Molecular Medicine Reports, 29, 107. https://doi.org/10.3892/mmr.2024.13231
MLA
Wang, C., Hu, M., Yu, K., Liu, W., Hu, A., Kuang, Y., Huang, L., Gajendran, B., Zacksenhaus, E., Xiao, X., Ben‑David, Y."An intricate regulatory circuit between FLI1 and GATA1/GATA2/LDB1/ERG dictates erythroid vs. megakaryocytic differentiation". Molecular Medicine Reports 29.6 (2024): 107.
Chicago
Wang, C., Hu, M., Yu, K., Liu, W., Hu, A., Kuang, Y., Huang, L., Gajendran, B., Zacksenhaus, E., Xiao, X., Ben‑David, Y."An intricate regulatory circuit between FLI1 and GATA1/GATA2/LDB1/ERG dictates erythroid vs. megakaryocytic differentiation". Molecular Medicine Reports 29, no. 6 (2024): 107. https://doi.org/10.3892/mmr.2024.13231
Copy and paste a formatted citation
x
Spandidos Publications style
Wang C, Hu M, Yu K, Liu W, Hu A, Kuang Y, Huang L, Gajendran B, Zacksenhaus E, Xiao X, Xiao X, et al: An intricate regulatory circuit between FLI1 and GATA1/GATA2/LDB1/ERG dictates erythroid vs. megakaryocytic differentiation. Mol Med Rep 29: 107, 2024.
APA
Wang, C., Hu, M., Yu, K., Liu, W., Hu, A., Kuang, Y. ... Ben‑David, Y. (2024). An intricate regulatory circuit between FLI1 and GATA1/GATA2/LDB1/ERG dictates erythroid vs. megakaryocytic differentiation. Molecular Medicine Reports, 29, 107. https://doi.org/10.3892/mmr.2024.13231
MLA
Wang, C., Hu, M., Yu, K., Liu, W., Hu, A., Kuang, Y., Huang, L., Gajendran, B., Zacksenhaus, E., Xiao, X., Ben‑David, Y."An intricate regulatory circuit between FLI1 and GATA1/GATA2/LDB1/ERG dictates erythroid vs. megakaryocytic differentiation". Molecular Medicine Reports 29.6 (2024): 107.
Chicago
Wang, C., Hu, M., Yu, K., Liu, W., Hu, A., Kuang, Y., Huang, L., Gajendran, B., Zacksenhaus, E., Xiao, X., Ben‑David, Y."An intricate regulatory circuit between FLI1 and GATA1/GATA2/LDB1/ERG dictates erythroid vs. megakaryocytic differentiation". Molecular Medicine Reports 29, no. 6 (2024): 107. https://doi.org/10.3892/mmr.2024.13231
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