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Semiquantitative analysis of protein expression in heated rat lens using shotgun proteomics

  • Authors:
    • Hiroko Otake
    • Shuya Masuda
    • Tetsushi Yamamoto
    • Yoshiki Miyata
    • Yosuke Nakazawa
    • Naoki Yamamoto
    • Atsushi Taga
    • Hiroshi Sasaki
    • Noriaki Nagai
  • View Affiliations / Copyright

    Affiliations: Faculty of Pharmacy, Kindai University, Higashiosaka, Osaka 577‑8502, Japan, Faculty of Pharmaceutical Sciences, Teikyo University, Tokyo 173‑8606, Japan, Faculty of Pharmacy, Keio University, Tokyo 105‑8512, Japan, Research Promotion Headquarters, Fujita Health University, Toyoake, Aichi 470‑1192, Japan, Department of Ophthalmology, Kanazawa Medical University, Uchinada, Ishikawa 920‑0293, Japan
    Copyright: © Otake et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 26
    |
    Published online on: November 11, 2024
       https://doi.org/10.3892/mmr.2024.13391
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Abstract

Previous studies have reported that a strong correlation between the estimated cumulative thermal exposure in the crystalline lens and the incidence of nuclear cataracts; however, the precise relationship between temperature and cataracts remains to be fully elucidated. In the present study, the shotgun liquid chromatography/mass spectroscopy‑based global proteomic approach was applied to investigate cataract‑inducing factors in lens cultured at normal (35.0˚C) and slightly warmer (37.5˚C) conditions. In the rat lens, 190 proteins (total) were identified. Of these, 48 proteins (25.3%) were found in lenses cultured at both 35.0˚C and 37.5˚C. Moreover, 85 proteins (44.7%) were unique to lenses cultured at 35.0˚C, while 57 proteins (30.0%) were unique to lenses cultured at 37.5˚C. Protein expression changes in rat lenses cultured at 37.5˚C were examined using a label‑free semiquantitative approach that uses spectral counting and Gene Ontology analysis. Filensin and vimentin protein expression, key factors in maintaining lens structure, were decreased. These findings may serve as a valuable indicator for elucidating the relationship between temperature and the onset of nuclear cataracts.
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Copy and paste a formatted citation
Spandidos Publications style
Otake H, Masuda S, Yamamoto T, Miyata Y, Nakazawa Y, Yamamoto N, Taga A, Sasaki H and Nagai N: Semiquantitative analysis of protein expression in heated rat lens using shotgun proteomics. Mol Med Rep 31: 26, 2025.
APA
Otake, H., Masuda, S., Yamamoto, T., Miyata, Y., Nakazawa, Y., Yamamoto, N. ... Nagai, N. (2025). Semiquantitative analysis of protein expression in heated rat lens using shotgun proteomics. Molecular Medicine Reports, 31, 26. https://doi.org/10.3892/mmr.2024.13391
MLA
Otake, H., Masuda, S., Yamamoto, T., Miyata, Y., Nakazawa, Y., Yamamoto, N., Taga, A., Sasaki, H., Nagai, N."Semiquantitative analysis of protein expression in heated rat lens using shotgun proteomics". Molecular Medicine Reports 31.1 (2025): 26.
Chicago
Otake, H., Masuda, S., Yamamoto, T., Miyata, Y., Nakazawa, Y., Yamamoto, N., Taga, A., Sasaki, H., Nagai, N."Semiquantitative analysis of protein expression in heated rat lens using shotgun proteomics". Molecular Medicine Reports 31, no. 1 (2025): 26. https://doi.org/10.3892/mmr.2024.13391
Copy and paste a formatted citation
x
Spandidos Publications style
Otake H, Masuda S, Yamamoto T, Miyata Y, Nakazawa Y, Yamamoto N, Taga A, Sasaki H and Nagai N: Semiquantitative analysis of protein expression in heated rat lens using shotgun proteomics. Mol Med Rep 31: 26, 2025.
APA
Otake, H., Masuda, S., Yamamoto, T., Miyata, Y., Nakazawa, Y., Yamamoto, N. ... Nagai, N. (2025). Semiquantitative analysis of protein expression in heated rat lens using shotgun proteomics. Molecular Medicine Reports, 31, 26. https://doi.org/10.3892/mmr.2024.13391
MLA
Otake, H., Masuda, S., Yamamoto, T., Miyata, Y., Nakazawa, Y., Yamamoto, N., Taga, A., Sasaki, H., Nagai, N."Semiquantitative analysis of protein expression in heated rat lens using shotgun proteomics". Molecular Medicine Reports 31.1 (2025): 26.
Chicago
Otake, H., Masuda, S., Yamamoto, T., Miyata, Y., Nakazawa, Y., Yamamoto, N., Taga, A., Sasaki, H., Nagai, N."Semiquantitative analysis of protein expression in heated rat lens using shotgun proteomics". Molecular Medicine Reports 31, no. 1 (2025): 26. https://doi.org/10.3892/mmr.2024.13391
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