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Article Open Access

Application of integrated omics in aseptic loosening of prostheses after hip replacement

  • Authors:
    • Yun-Ke Liu
    • Yong-Hui Dong
    • Xia-Ming Liang
    • Shuo Qiang
    • Meng-En Li
    • Zhuang Sun
    • Xin Zhao
    • Zhi-Hua Yan
    • Jia Zheng
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedics, People's Hospital of Zhengzhou University, Zhengzhou, Henan 450003, P.R. China
    Copyright: © Liu et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 65
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    Published online on: January 3, 2025
       https://doi.org/10.3892/mmr.2025.13430
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Abstract

Aseptic loosening (AL) of artificial hip joints is the most common complication following hip replacement surgery. A total of eight patients diagnosed with AL following total hip arthroplasty (THA) undergoing total hip replacement and eight control patients diagnosed with avascular necrosis of femoral head (ANFH) or femoral neck fracture undergoing THA were enrolled. The samples of the AL group were from synovial tissue surrounding the lining/head/neck of the prosthesis, and the samples of the control group were from the synovium in the joint cavity. The present study utilized second‑generation high‑throughput sequencing and mass spectrometry to detect differentially expressed genes, proteins and metabolites in the samples, as well as Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analysis. Key genes cytokine receptor‑like factor‑1 (CRLF1) and glutathione‑S transferase µ1 (GSTM1) expression levels were verified by reverse transcription‑quantitative PCR and western blotting. The integrated transcriptomics, proteomics and untargeted metabolomics analyses revealed characteristic metabolite changes (biosynthesis of guanine, L‑glycine and adenosine) and decreased CRLF1 and GSTM1 in AL, which were primarily associated with amino acid metabolism and lipid metabolism. In summary, the present study may uncover the underlying mechanisms of AL pathology and provide stable and accurate biomarkers for early warning and diagnosis.
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Copy and paste a formatted citation
Spandidos Publications style
Liu Y, Dong Y, Liang X, Qiang S, Li M, Sun Z, Zhao X, Yan Z and Zheng J: Application of integrated omics in aseptic loosening of prostheses after hip replacement. Mol Med Rep 31: 65, 2025.
APA
Liu, Y., Dong, Y., Liang, X., Qiang, S., Li, M., Sun, Z. ... Zheng, J. (2025). Application of integrated omics in aseptic loosening of prostheses after hip replacement. Molecular Medicine Reports, 31, 65. https://doi.org/10.3892/mmr.2025.13430
MLA
Liu, Y., Dong, Y., Liang, X., Qiang, S., Li, M., Sun, Z., Zhao, X., Yan, Z., Zheng, J."Application of integrated omics in aseptic loosening of prostheses after hip replacement". Molecular Medicine Reports 31.3 (2025): 65.
Chicago
Liu, Y., Dong, Y., Liang, X., Qiang, S., Li, M., Sun, Z., Zhao, X., Yan, Z., Zheng, J."Application of integrated omics in aseptic loosening of prostheses after hip replacement". Molecular Medicine Reports 31, no. 3 (2025): 65. https://doi.org/10.3892/mmr.2025.13430
Copy and paste a formatted citation
x
Spandidos Publications style
Liu Y, Dong Y, Liang X, Qiang S, Li M, Sun Z, Zhao X, Yan Z and Zheng J: Application of integrated omics in aseptic loosening of prostheses after hip replacement. Mol Med Rep 31: 65, 2025.
APA
Liu, Y., Dong, Y., Liang, X., Qiang, S., Li, M., Sun, Z. ... Zheng, J. (2025). Application of integrated omics in aseptic loosening of prostheses after hip replacement. Molecular Medicine Reports, 31, 65. https://doi.org/10.3892/mmr.2025.13430
MLA
Liu, Y., Dong, Y., Liang, X., Qiang, S., Li, M., Sun, Z., Zhao, X., Yan, Z., Zheng, J."Application of integrated omics in aseptic loosening of prostheses after hip replacement". Molecular Medicine Reports 31.3 (2025): 65.
Chicago
Liu, Y., Dong, Y., Liang, X., Qiang, S., Li, M., Sun, Z., Zhao, X., Yan, Z., Zheng, J."Application of integrated omics in aseptic loosening of prostheses after hip replacement". Molecular Medicine Reports 31, no. 3 (2025): 65. https://doi.org/10.3892/mmr.2025.13430
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