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Protective effect of soluble protein hydrolysate against H2O2‑induced intestinal injury: An interventional study

  • Authors:
    • Jingjing Wei
    • Guozhong Tao
    • Junlin Liu
    • Bomi Framroze
    • Karl G. Sylvester
  • View Affiliations / Copyright

    Affiliations: Department of Surgery, Stanford University School of Medicine, Stanford, CA 94304, USA, Department of R&D, Hofseth BioCare ASA, Aalesund 6003, Norway
    Copyright: © Wei et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 85
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    Published online on: January 29, 2025
       https://doi.org/10.3892/mmr.2025.13450
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Abstract

The present study aimed to investigate whether soluble protein hydrolysate (SPH) protects against intestinal oxidative stress injury. An in vitro lactate dehydrogenase assay was used to assess the cytotoxicity and protective effect of SPH. For in vivo assessment, friend virus B NIH Jackson mouse pups aged 21 days were administered with 5% w/v soluble protein hydrolysate (SPH) through drinking water for 14 days and then luminally injected with 0.3% or 0.6% H2O2. Thereafter, the fecal samples of mice were collected, and the mice were sacrificed. Intestinal epithelial injury was assessed, and the expressions of 84 oxidative stress‑related genes in intestinal tissues was determined. SPH prophylactically protected against H2O2‑induced oxidative stress injury in human intestinal epithelial cells. An animal model of oxidative stress‑induced intestinal injury was established using 0.3 and 0.6% H2O2. SPH treatment reduced oxidative stress (0.3% H2O2)‑induced gut injury in mice. As no accelerated body growth was observed in SPH‑treated mice, it was hypothesized that the underlying protective mechanism of SPH is not related to nutrient oversupply. Treatment with SPH upregulated five oxidative protective genes that were not consistent between the sexes. Some antioxidative genes, including ferritin heavy polypeptide‑1 (Fth1), heme oxygenase‑1 (Hmox1), NAD(P)H dehydrogenase quinone 1 (Nqo1) and superoxide dismutase 1 (Sod1), were commonly upregulated in both male and female mice. Overall, an antioxidative protective effect was observed following SPH treatment, which may be attributed to the upregulation of genes that protect against oxidative damage. The findings of the present study highlight the promising potential of SPH as a functional food for alleviating intestinal oxidative stress injury.
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Copy and paste a formatted citation
Spandidos Publications style
Wei J, Tao G, Liu J, Framroze B and Sylvester KG: Protective effect of soluble protein hydrolysate against H<sub>2</sub>O<sub>2</sub>‑induced intestinal injury: An interventional study. Mol Med Rep 31: 85, 2025.
APA
Wei, J., Tao, G., Liu, J., Framroze, B., & Sylvester, K.G. (2025). Protective effect of soluble protein hydrolysate against H<sub>2</sub>O<sub>2</sub>‑induced intestinal injury: An interventional study. Molecular Medicine Reports, 31, 85. https://doi.org/10.3892/mmr.2025.13450
MLA
Wei, J., Tao, G., Liu, J., Framroze, B., Sylvester, K. G."Protective effect of soluble protein hydrolysate against H<sub>2</sub>O<sub>2</sub>‑induced intestinal injury: An interventional study". Molecular Medicine Reports 31.4 (2025): 85.
Chicago
Wei, J., Tao, G., Liu, J., Framroze, B., Sylvester, K. G."Protective effect of soluble protein hydrolysate against H<sub>2</sub>O<sub>2</sub>‑induced intestinal injury: An interventional study". Molecular Medicine Reports 31, no. 4 (2025): 85. https://doi.org/10.3892/mmr.2025.13450
Copy and paste a formatted citation
x
Spandidos Publications style
Wei J, Tao G, Liu J, Framroze B and Sylvester KG: Protective effect of soluble protein hydrolysate against H<sub>2</sub>O<sub>2</sub>‑induced intestinal injury: An interventional study. Mol Med Rep 31: 85, 2025.
APA
Wei, J., Tao, G., Liu, J., Framroze, B., & Sylvester, K.G. (2025). Protective effect of soluble protein hydrolysate against H<sub>2</sub>O<sub>2</sub>‑induced intestinal injury: An interventional study. Molecular Medicine Reports, 31, 85. https://doi.org/10.3892/mmr.2025.13450
MLA
Wei, J., Tao, G., Liu, J., Framroze, B., Sylvester, K. G."Protective effect of soluble protein hydrolysate against H<sub>2</sub>O<sub>2</sub>‑induced intestinal injury: An interventional study". Molecular Medicine Reports 31.4 (2025): 85.
Chicago
Wei, J., Tao, G., Liu, J., Framroze, B., Sylvester, K. G."Protective effect of soluble protein hydrolysate against H<sub>2</sub>O<sub>2</sub>‑induced intestinal injury: An interventional study". Molecular Medicine Reports 31, no. 4 (2025): 85. https://doi.org/10.3892/mmr.2025.13450
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