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LncPrep + 96kb inhibits ovarian fibrosis by upregulating prolyl oligopeptidase expression

  • Authors:
    • Hongdan Zhang
    • Jing Wang
    • Jianwei Liu
    • Xiang Fan
    • Yinuo Jia
    • Yingtong Huang
    • Qihui Han
    • Shimeng Wang
    • Li Xiao
    • Xiang Li
    • Chunping Zhang
  • View Affiliations / Copyright

    Affiliations: School of Basic Medical Sciences, Jiangxi Medical College, Nanchang University, Nanchang, Jiangxi 330019, P.R. China
    Copyright: © Zhang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 113
    |
    Published online on: February 26, 2025
       https://doi.org/10.3892/mmr.2025.13478
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Abstract

LncPrep + 96kb is a long non‑coding RNA expressed in murine granulosa cells. The 2.2-kb fragment of lncPrep + 96kb inhibits aromatase expression and estrogen secretion in ovarian granulosa cells. In the present study, lncPrep + 96kb‑knockout (KO) mice were generated, and significant ovarian fibrosis and reduced female fertility through fertility monitoring and superovulation. The augmentation of ovarian fibrosis was observed by Sirius red staining and western blot and RT‑qPCR. Notably, lncPrep + 96kb was identified in conserved non‑coding sequences adjacent to the prolyl oligopeptidase (POP) gene. Furthermore, POP expression was shown to be reduced in lncPrep + 96kb‑KO mice, whereas overexpression of lncPrep + 96kb increased POP expression. Further studies revealed that POP regulated the expression levels of factors related to fibrosis, including matrix metalloproteinase 2 (MMP2), transforming growth factor β1 (TGF‑β1) and peroxisome proliferator activated receptor γ (PPAR‑γ). In conclusion, ovarian fibrosis was elevated in lncPrep + 96kb‑KO mice, and POP may act as a target of lncPrep + 96kb, which mediates ovarian fibrosis through the regulation of PPAR‑γ, MMP2 and TGF‑β1 expression. 
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Zhang H, Wang J, Liu J, Fan X, Jia Y, Huang Y, Han Q, Wang S, Xiao L, Li X, Li X, et al: LncPrep + 96kb inhibits ovarian fibrosis by upregulating prolyl oligopeptidase expression. Mol Med Rep 31: 113, 2025.
APA
Zhang, H., Wang, J., Liu, J., Fan, X., Jia, Y., Huang, Y. ... Zhang, C. (2025). LncPrep + 96kb inhibits ovarian fibrosis by upregulating prolyl oligopeptidase expression. Molecular Medicine Reports, 31, 113. https://doi.org/10.3892/mmr.2025.13478
MLA
Zhang, H., Wang, J., Liu, J., Fan, X., Jia, Y., Huang, Y., Han, Q., Wang, S., Xiao, L., Li, X., Zhang, C."LncPrep + 96kb inhibits ovarian fibrosis by upregulating prolyl oligopeptidase expression". Molecular Medicine Reports 31.5 (2025): 113.
Chicago
Zhang, H., Wang, J., Liu, J., Fan, X., Jia, Y., Huang, Y., Han, Q., Wang, S., Xiao, L., Li, X., Zhang, C."LncPrep + 96kb inhibits ovarian fibrosis by upregulating prolyl oligopeptidase expression". Molecular Medicine Reports 31, no. 5 (2025): 113. https://doi.org/10.3892/mmr.2025.13478
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang H, Wang J, Liu J, Fan X, Jia Y, Huang Y, Han Q, Wang S, Xiao L, Li X, Li X, et al: LncPrep + 96kb inhibits ovarian fibrosis by upregulating prolyl oligopeptidase expression. Mol Med Rep 31: 113, 2025.
APA
Zhang, H., Wang, J., Liu, J., Fan, X., Jia, Y., Huang, Y. ... Zhang, C. (2025). LncPrep + 96kb inhibits ovarian fibrosis by upregulating prolyl oligopeptidase expression. Molecular Medicine Reports, 31, 113. https://doi.org/10.3892/mmr.2025.13478
MLA
Zhang, H., Wang, J., Liu, J., Fan, X., Jia, Y., Huang, Y., Han, Q., Wang, S., Xiao, L., Li, X., Zhang, C."LncPrep + 96kb inhibits ovarian fibrosis by upregulating prolyl oligopeptidase expression". Molecular Medicine Reports 31.5 (2025): 113.
Chicago
Zhang, H., Wang, J., Liu, J., Fan, X., Jia, Y., Huang, Y., Han, Q., Wang, S., Xiao, L., Li, X., Zhang, C."LncPrep + 96kb inhibits ovarian fibrosis by upregulating prolyl oligopeptidase expression". Molecular Medicine Reports 31, no. 5 (2025): 113. https://doi.org/10.3892/mmr.2025.13478
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