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Article Open Access

RNA‑seq analysis of predictive markers associated with glutamine metabolism in thyroid cancer

  • Authors:
    • Yi You
    • Yuheng Zhou
    • Zilu Chen
    • Longcheng Deng
    • Yaping Shen
    • Qin Wang
    • Wei Long
    • Yan Xiong
    • Foxing Tan
    • Haolin Du
    • Yan Yang
    • Jiang Zhong
    • Yunqian Ge
    • Youchen Li
    • Yan Huang
  • View Affiliations / Copyright

    Affiliations: Department of Ultrasound, Nanjing Hospital of Chinese Medicine Affiliated to Nanjing University of Chinese Medicine, Nanjing, Jiangsu 210022, P.R. China
    Copyright: © You et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 145
    |
    Published online on: April 1, 2025
       https://doi.org/10.3892/mmr.2025.13510
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Abstract

The incidence of thyroid cancer (TC) increases year by year. It is necessary to construct a prognostic model for risk stratification and management of TC patients. Glutamine metabolism is essential for tumor progression and the tumor microenvironment. The present study aimed to develop a predictive model for TC using a glutamine metabolism gene set. Differentially expressed genes in cells with high glutamine metabolism levels from single cell RNA‑sequencing data were compared with genes differentially expressed between normal and TC tissues from The Cancer Genome Atlas Program data. Through Boruta feature selection methods and multivariate Cox regression, six crucial genes were identified for a risk‑scoring system to develop a prognostic model. The role of each gene was verified in TC cells in vitro. A risk‑scoring system was developed according to the glutamine gene set to forecast the overall survival of TC patients. This risk score could stratify TC patients and minimize unnecessary surgeries and invasive treatments. In addition, signal induced proliferation associated 1 like 2 (SIPA1L2), an important gene in the prognostic model, knockdown in TPC‑1 and BCPAP cell lines enhanced TC cell proliferation, migration and invasion. A risk model was developed based on a glutamine metabolism gene set. The model has reference values for TC stratification.
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Copy and paste a formatted citation
Spandidos Publications style
You Y, Zhou Y, Chen Z, Deng L, Shen Y, Wang Q, Long W, Xiong Y, Tan F, Du H, Du H, et al: RNA‑seq analysis of predictive markers associated with glutamine metabolism in thyroid cancer. Mol Med Rep 31: 145, 2025.
APA
You, Y., Zhou, Y., Chen, Z., Deng, L., Shen, Y., Wang, Q. ... Huang, Y. (2025). RNA‑seq analysis of predictive markers associated with glutamine metabolism in thyroid cancer. Molecular Medicine Reports, 31, 145. https://doi.org/10.3892/mmr.2025.13510
MLA
You, Y., Zhou, Y., Chen, Z., Deng, L., Shen, Y., Wang, Q., Long, W., Xiong, Y., Tan, F., Du, H., Yang, Y., Zhong, J., Ge, Y., Li, Y., Huang, Y."RNA‑seq analysis of predictive markers associated with glutamine metabolism in thyroid cancer". Molecular Medicine Reports 31.6 (2025): 145.
Chicago
You, Y., Zhou, Y., Chen, Z., Deng, L., Shen, Y., Wang, Q., Long, W., Xiong, Y., Tan, F., Du, H., Yang, Y., Zhong, J., Ge, Y., Li, Y., Huang, Y."RNA‑seq analysis of predictive markers associated with glutamine metabolism in thyroid cancer". Molecular Medicine Reports 31, no. 6 (2025): 145. https://doi.org/10.3892/mmr.2025.13510
Copy and paste a formatted citation
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Spandidos Publications style
You Y, Zhou Y, Chen Z, Deng L, Shen Y, Wang Q, Long W, Xiong Y, Tan F, Du H, Du H, et al: RNA‑seq analysis of predictive markers associated with glutamine metabolism in thyroid cancer. Mol Med Rep 31: 145, 2025.
APA
You, Y., Zhou, Y., Chen, Z., Deng, L., Shen, Y., Wang, Q. ... Huang, Y. (2025). RNA‑seq analysis of predictive markers associated with glutamine metabolism in thyroid cancer. Molecular Medicine Reports, 31, 145. https://doi.org/10.3892/mmr.2025.13510
MLA
You, Y., Zhou, Y., Chen, Z., Deng, L., Shen, Y., Wang, Q., Long, W., Xiong, Y., Tan, F., Du, H., Yang, Y., Zhong, J., Ge, Y., Li, Y., Huang, Y."RNA‑seq analysis of predictive markers associated with glutamine metabolism in thyroid cancer". Molecular Medicine Reports 31.6 (2025): 145.
Chicago
You, Y., Zhou, Y., Chen, Z., Deng, L., Shen, Y., Wang, Q., Long, W., Xiong, Y., Tan, F., Du, H., Yang, Y., Zhong, J., Ge, Y., Li, Y., Huang, Y."RNA‑seq analysis of predictive markers associated with glutamine metabolism in thyroid cancer". Molecular Medicine Reports 31, no. 6 (2025): 145. https://doi.org/10.3892/mmr.2025.13510
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