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Multifunctional cosmetic potential of extracellular vesicle‑like nanoparticles derived from the stem of Cannabis sativa in treating pigmentation disorders

  • Authors:
    • Hyeon Jin Lee
    • Yun Hye Kim
    • Seo Jun Lee
    • Su Hyun Park
    • Jae-Min Yuk
    • Jae Cheol Jeong
    • Young Bae Ryu
    • Woo Sik Kim
  • View Affiliations / Copyright

    Affiliations: Functional Biomaterial Research Center, Korea Research Institute of Bioscience and Biotechnology, Jeongeup, North Jeolla 56212, Republic of Korea, Department of Medical Science, College of Medicine, Chungnam National University, Daejeon, South Chungcheong 35015, Republic of Korea, Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology, Jeongeup, North Jeolla 56212, Republic of Korea
    Copyright: © Lee et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 147
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    Published online on: April 1, 2025
       https://doi.org/10.3892/mmr.2025.13512
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Abstract

While natural products and synthetic chemicals are used in functional cosmetics, their potential side effects remain a concern. This has driven the need for safer and more effective agents to treat skin disorders. This has driven the need safer and more effective agents to treat skin disorders. Therefore, the present study aimed to explore the functional properties of Cannabis sativa stem‑derived nanoparticles (CSS‑NPs) and evaluate their potential as a cosmetic ingredient. Using nanoparticle analysis, CSS‑NPs, with a mean diameter of ~120 nm exhibited notable resistance to external stress conditions, including pH fluctuation and enzymatic degradation by DNase, RNase and proteinase K. They also contained 48 distinct biochemical components. In vitro assays revealed that CSS‑NPs significantly downregulated the expression of genes and proteins associated with melanin synthesis in mouse B16F10 melanoma cells under α‑melanocyte stimulating hormone (α‑MSH)‑induced hyperpigmentation. These inhibitory effects were mediated by the activation of ERK and Akt signaling pathways. Furthermore, CSS‑NPs improved the viability of α‑MSH‑treated B16F10 cells; this was accompanied by the upregulation of antioxidant‑associated enzymes and a decrease in α‑MSH‑induced reactive oxygen species levels. Collectively, these findings suggested that CSS‑NPs carry out a key role in mitigating skin pigmentation and enhancing antioxidant defenses by modulating the ERK/Akt axis during excessive melanin synthesis. Thus, CSS‑NPs represent a promising multifunctional cosmetic ingredient with potential in treating pigmentation disorders and protecting skin cells.
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Copy and paste a formatted citation
Spandidos Publications style
Lee HJ, Kim YH, Lee SJ, Park SH, Yuk J, Jeong JC, Ryu YB and Kim WS: Multifunctional cosmetic potential of extracellular vesicle‑like nanoparticles derived from the stem of <em>Cannabis sativa</em> in treating pigmentation disorders. Mol Med Rep 31: 147, 2025.
APA
Lee, H.J., Kim, Y.H., Lee, S.J., Park, S.H., Yuk, J., Jeong, J.C. ... Kim, W.S. (2025). Multifunctional cosmetic potential of extracellular vesicle‑like nanoparticles derived from the stem of <em>Cannabis sativa</em> in treating pigmentation disorders. Molecular Medicine Reports, 31, 147. https://doi.org/10.3892/mmr.2025.13512
MLA
Lee, H. J., Kim, Y. H., Lee, S. J., Park, S. H., Yuk, J., Jeong, J. C., Ryu, Y. B., Kim, W. S."Multifunctional cosmetic potential of extracellular vesicle‑like nanoparticles derived from the stem of <em>Cannabis sativa</em> in treating pigmentation disorders". Molecular Medicine Reports 31.6 (2025): 147.
Chicago
Lee, H. J., Kim, Y. H., Lee, S. J., Park, S. H., Yuk, J., Jeong, J. C., Ryu, Y. B., Kim, W. S."Multifunctional cosmetic potential of extracellular vesicle‑like nanoparticles derived from the stem of <em>Cannabis sativa</em> in treating pigmentation disorders". Molecular Medicine Reports 31, no. 6 (2025): 147. https://doi.org/10.3892/mmr.2025.13512
Copy and paste a formatted citation
x
Spandidos Publications style
Lee HJ, Kim YH, Lee SJ, Park SH, Yuk J, Jeong JC, Ryu YB and Kim WS: Multifunctional cosmetic potential of extracellular vesicle‑like nanoparticles derived from the stem of <em>Cannabis sativa</em> in treating pigmentation disorders. Mol Med Rep 31: 147, 2025.
APA
Lee, H.J., Kim, Y.H., Lee, S.J., Park, S.H., Yuk, J., Jeong, J.C. ... Kim, W.S. (2025). Multifunctional cosmetic potential of extracellular vesicle‑like nanoparticles derived from the stem of <em>Cannabis sativa</em> in treating pigmentation disorders. Molecular Medicine Reports, 31, 147. https://doi.org/10.3892/mmr.2025.13512
MLA
Lee, H. J., Kim, Y. H., Lee, S. J., Park, S. H., Yuk, J., Jeong, J. C., Ryu, Y. B., Kim, W. S."Multifunctional cosmetic potential of extracellular vesicle‑like nanoparticles derived from the stem of <em>Cannabis sativa</em> in treating pigmentation disorders". Molecular Medicine Reports 31.6 (2025): 147.
Chicago
Lee, H. J., Kim, Y. H., Lee, S. J., Park, S. H., Yuk, J., Jeong, J. C., Ryu, Y. B., Kim, W. S."Multifunctional cosmetic potential of extracellular vesicle‑like nanoparticles derived from the stem of <em>Cannabis sativa</em> in treating pigmentation disorders". Molecular Medicine Reports 31, no. 6 (2025): 147. https://doi.org/10.3892/mmr.2025.13512
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