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Natural bioactive gallic acid shows potential anticancer effects by inhibiting the proliferation and invasiveness behavior in human embryonic carcinoma cells

  • Authors:
    • Dong Young Kang
    • Se Won Bae
    • Kyoung-Jin Jang
  • View Affiliations / Copyright

    Affiliations: Department of Immunology, School of Medicine, Institute of Biomedical Science and Technology, Konkuk University, Chungju. Chungcheong 27478, Republic of Korea, Department of Chemistry and Cosmetics, Jeju National University, Jeju, Jejudo 63243, Republic of Korea, Department of Integrative Biological Sciences and Industry, College of Life Science, Sejong University, Seoul 05006, Republic of Korea
    Copyright: © Kang et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 151
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    Published online on: April 4, 2025
       https://doi.org/10.3892/mmr.2025.13516
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Abstract

Embryonic cancer stem cells (CSCs), referred to as self‑renewable cells, are commonly found in liquid and solid cancers and can also be attributed to tumor onset, resistance, expansion, recurrence and metastasis following treatment. Cancer therapy targeting CSCs using natural bioactive products is an optimal option for inhibiting cancer recurrence, thereby improving prognosis. Several natural compounds and extracts have been used to identify direct or indirect therapy effects that reduce the pathological activities of CSCs. Natural gallic acid (GA) is noted to have anticancer properties for oncogene expression, cycle arrest, apoptosis, angiogenesis, migration and metastasis in various cancers. The present study demonstrated that GA has various anticancer activities in NTERA‑2 and NCCIT human embryonic carcinoma cells. In two types of embryonic CSCs, GA effectively induced cell death via late apoptosis. Furthermore, GA showed the G0/G1 cell cycle arrest activity in embryonic CSCs by inducing the increase of p21, p27 and p53 expression and the decrease of CDK4, cyclin E and cyclin D1 expression. The present study showed that GA inhibited the expression levels of mRNA and protein for stem cell markers, such as SOX2, NANOG and OCT4, in NTERA‑2 and NCCIT cells. The induction of cellular and mitochondrial reactive oxygen species by GA also activated the cellular DNA damage response pathway by raising the phosphorylated‑BRCA1, ATM, Chk1, Chk2 and histone. Finally, GA inhibited CSCs invasion and migration by inhibiting the expression of matrix metalloproteinase by the downregulation of EGFR/JAK2/STAT5 signaling pathway. Thus, it is hypothesized that GA could be a potential inhibitor of cancer emergence by suppressing CSC properties.
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Copy and paste a formatted citation
Spandidos Publications style
Kang D, Bae S and Jang K: Natural bioactive gallic acid shows potential anticancer effects by inhibiting the proliferation and invasiveness behavior in human embryonic carcinoma cells. Mol Med Rep 31: 151, 2025.
APA
Kang, D., Bae, S., & Jang, K. (2025). Natural bioactive gallic acid shows potential anticancer effects by inhibiting the proliferation and invasiveness behavior in human embryonic carcinoma cells. Molecular Medicine Reports, 31, 151. https://doi.org/10.3892/mmr.2025.13516
MLA
Kang, D., Bae, S., Jang, K."Natural bioactive gallic acid shows potential anticancer effects by inhibiting the proliferation and invasiveness behavior in human embryonic carcinoma cells". Molecular Medicine Reports 31.6 (2025): 151.
Chicago
Kang, D., Bae, S., Jang, K."Natural bioactive gallic acid shows potential anticancer effects by inhibiting the proliferation and invasiveness behavior in human embryonic carcinoma cells". Molecular Medicine Reports 31, no. 6 (2025): 151. https://doi.org/10.3892/mmr.2025.13516
Copy and paste a formatted citation
x
Spandidos Publications style
Kang D, Bae S and Jang K: Natural bioactive gallic acid shows potential anticancer effects by inhibiting the proliferation and invasiveness behavior in human embryonic carcinoma cells. Mol Med Rep 31: 151, 2025.
APA
Kang, D., Bae, S., & Jang, K. (2025). Natural bioactive gallic acid shows potential anticancer effects by inhibiting the proliferation and invasiveness behavior in human embryonic carcinoma cells. Molecular Medicine Reports, 31, 151. https://doi.org/10.3892/mmr.2025.13516
MLA
Kang, D., Bae, S., Jang, K."Natural bioactive gallic acid shows potential anticancer effects by inhibiting the proliferation and invasiveness behavior in human embryonic carcinoma cells". Molecular Medicine Reports 31.6 (2025): 151.
Chicago
Kang, D., Bae, S., Jang, K."Natural bioactive gallic acid shows potential anticancer effects by inhibiting the proliferation and invasiveness behavior in human embryonic carcinoma cells". Molecular Medicine Reports 31, no. 6 (2025): 151. https://doi.org/10.3892/mmr.2025.13516
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