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Antcin K inhibits chondrosarcoma motility by reducing MMP‑7 expression via downregulation of the PI3K, Akt, mTOR and NF‑κB signaling pathway

  • Authors:
    • Yat-Yin Law
    • Nguyen Bao Tran
    • Chang-Yu Song
    • Yu-Ying Wu
    • Hsien-Te Chen
    • Yi-Chin Fong
    • Hsiao-Chi Tsai
    • Yueh-Hsiung Kuo
    • Chih-Hsin Tang
  • View Affiliations / Copyright

    Affiliations: School of Medicine, Chung Shan Medical University, Taichung, Taiwan 40201, Taiwan, R.O.C., Graduate Institute of Biomedical Sciences, China Medical University, Taichung, Taiwan 404, Taiwan, R.O.C., Department of Sports Medicine, College of Health Care, China Medical University, Taichung, Taiwan 404, Taiwan, R.O.C., Department of Medicine Research, China Medical University Beigang Hospital, Yunlin, Taiwan 651, Taiwan, R.O.C., Department of Chinese Pharmaceutical Sciences and Chinese Medicine Resources, China Medical University, Taichung, Taiwan 404, Taiwan, R.O.C.
    Copyright: © Law et al. This is an open access article distributed under the terms of Creative Commons Attribution License.
  • Article Number: 180
    |
    Published online on: April 23, 2025
       https://doi.org/10.3892/mmr.2025.13545
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Abstract

Chondrosarcoma is the second most common form of primary bone cancer originating from cartilage. Chondrosarcoma cells have a high propensity to spread to other organs during the advanced stage, with the lung being a preferred site. Although surgery is the most effective treatment for chondrosarcoma, it has low efficacy in the metastasis stage. Antrodia cinnamomea is the source of the triterpenoid antcin K, which exhibits immunomodulatory and anti‑inflammatory properties. However, the therapeutic function of antcin K on chondrosarcoma has not yet been elucidated. The inhibitory effect of antcin K was evaluated using migration and invasion assays while cell toxicity was determined using the MTT assay. Molecular function regulation by antcin K was investigated by RNA sequencing and Ingenuity Pathway Analysis. The present study revealed that antcin K decreases migration and invasion in two chondrosarcoma cell lines. RNA sequencing revealed that MMP‑7 serves a key role in antcin K‑mediated motility of chondrosarcoma cells. Antcin K diminished MMP‑7 expression, and overexpression of MMP‑7 antagonized antcin K‑induced inhibition of cell migration and invasion. Antcin K abolished the activation of PI3K, Akt, mTOR and NF‑κB pathways. The present study demonstrated that antcin K is a novel candidate for chondrosarcoma motility inhibition by decreasing the PI3K, Akt, mTOR and NF‑κB signaling cascades, which inhibits MMP‑7 production.
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Spandidos Publications style
Law Y, Tran NB, Song C, Wu Y, Chen H, Fong Y, Tsai H, Kuo Y and Tang C: Antcin K inhibits chondrosarcoma motility by reducing MMP‑7 expression via downregulation of the PI3K, Akt, mTOR and NF‑κB signaling pathway. Mol Med Rep 32: 180, 2025.
APA
Law, Y., Tran, N.B., Song, C., Wu, Y., Chen, H., Fong, Y. ... Tang, C. (2025). Antcin K inhibits chondrosarcoma motility by reducing MMP‑7 expression via downregulation of the PI3K, Akt, mTOR and NF‑κB signaling pathway. Molecular Medicine Reports, 32, 180. https://doi.org/10.3892/mmr.2025.13545
MLA
Law, Y., Tran, N. B., Song, C., Wu, Y., Chen, H., Fong, Y., Tsai, H., Kuo, Y., Tang, C."Antcin K inhibits chondrosarcoma motility by reducing MMP‑7 expression via downregulation of the PI3K, Akt, mTOR and NF‑κB signaling pathway". Molecular Medicine Reports 32.1 (2025): 180.
Chicago
Law, Y., Tran, N. B., Song, C., Wu, Y., Chen, H., Fong, Y., Tsai, H., Kuo, Y., Tang, C."Antcin K inhibits chondrosarcoma motility by reducing MMP‑7 expression via downregulation of the PI3K, Akt, mTOR and NF‑κB signaling pathway". Molecular Medicine Reports 32, no. 1 (2025): 180. https://doi.org/10.3892/mmr.2025.13545
Copy and paste a formatted citation
x
Spandidos Publications style
Law Y, Tran NB, Song C, Wu Y, Chen H, Fong Y, Tsai H, Kuo Y and Tang C: Antcin K inhibits chondrosarcoma motility by reducing MMP‑7 expression via downregulation of the PI3K, Akt, mTOR and NF‑κB signaling pathway. Mol Med Rep 32: 180, 2025.
APA
Law, Y., Tran, N.B., Song, C., Wu, Y., Chen, H., Fong, Y. ... Tang, C. (2025). Antcin K inhibits chondrosarcoma motility by reducing MMP‑7 expression via downregulation of the PI3K, Akt, mTOR and NF‑κB signaling pathway. Molecular Medicine Reports, 32, 180. https://doi.org/10.3892/mmr.2025.13545
MLA
Law, Y., Tran, N. B., Song, C., Wu, Y., Chen, H., Fong, Y., Tsai, H., Kuo, Y., Tang, C."Antcin K inhibits chondrosarcoma motility by reducing MMP‑7 expression via downregulation of the PI3K, Akt, mTOR and NF‑κB signaling pathway". Molecular Medicine Reports 32.1 (2025): 180.
Chicago
Law, Y., Tran, N. B., Song, C., Wu, Y., Chen, H., Fong, Y., Tsai, H., Kuo, Y., Tang, C."Antcin K inhibits chondrosarcoma motility by reducing MMP‑7 expression via downregulation of the PI3K, Akt, mTOR and NF‑κB signaling pathway". Molecular Medicine Reports 32, no. 1 (2025): 180. https://doi.org/10.3892/mmr.2025.13545
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