A retrospective study on TS mRNA expression and prediction of the effects of adjuvant oral 5-fluorouracil in breast cancer

  • Authors:
    • Fuminori Aki
    • Yoshimi Bando
    • Tetsuyuki Takahashi
    • Hisanori Uehara
    • Satoshi Numoto
    • Sueyoshi Ito
    • Mitsunori Sasa
    • Keisuke Izumi
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  • Published online on: September 23, 2010     https://doi.org/10.3892/ol.2010.186
  • Pages: 981-988
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Abstract

Nucleic acid-metabolizing enzymes, such as thymidylate synthase (TS), dihydropyrimidine dehydrogenase (DPD), thymidine phosphorylase (TP) and orotate phosphoribosyl transferase (OPRT), have attracted attention as candidates for response determinants of 5-fluorouracil (5-FU). Whether the expression levels of these enzymes can be adopted as valuable parameters for 5-FU sensitivity in breast cancer has yet to be elucidated. In the present study, intratumoral mRNA expression of TS, DPD, TP and OPRT were determined in formalin-fixed paraffin-embedded surgical specimens collected from 217 breast cancer patients, using the Danenberg Tumor Profile method, which combines microdissection and real-time-polymerase chain reaction. The significance of these enzymes as prognostic and 5-FU efficacy-predicting factors was evaluated. Our data showed that a low DPD expression is related to a high nuclear grade and other factors including hormone receptor-negativity. Low expression levels of TP were found in hormone receptor-negative tumors. TS and OPRT expression were not related to various clinicopathological factors, but patients with a high TS mRNA expression showed a significantly poorer prognosis in cases where 5-FU was not administered. The efficacy of 5-FU was more significant when administered for more than 6 months in the group with a high TS mRNA expression. These data suggest that TS mRNA expression in breast cancer tissue is an ideal predictor of outcomes for patients with no administration of 5-FU, and of the efficacy of 5-FU.
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November-December 2010
Volume 1 Issue 6

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Aki F, Bando Y, Takahashi T, Uehara H, Numoto S, Ito S, Sasa M and Izumi K: A retrospective study on TS mRNA expression and prediction of the effects of adjuvant oral 5-fluorouracil in breast cancer. Oncol Lett 1: 981-988, 2010
APA
Aki, F., Bando, Y., Takahashi, T., Uehara, H., Numoto, S., Ito, S. ... Izumi, K. (2010). A retrospective study on TS mRNA expression and prediction of the effects of adjuvant oral 5-fluorouracil in breast cancer. Oncology Letters, 1, 981-988. https://doi.org/10.3892/ol.2010.186
MLA
Aki, F., Bando, Y., Takahashi, T., Uehara, H., Numoto, S., Ito, S., Sasa, M., Izumi, K."A retrospective study on TS mRNA expression and prediction of the effects of adjuvant oral 5-fluorouracil in breast cancer". Oncology Letters 1.6 (2010): 981-988.
Chicago
Aki, F., Bando, Y., Takahashi, T., Uehara, H., Numoto, S., Ito, S., Sasa, M., Izumi, K."A retrospective study on TS mRNA expression and prediction of the effects of adjuvant oral 5-fluorouracil in breast cancer". Oncology Letters 1, no. 6 (2010): 981-988. https://doi.org/10.3892/ol.2010.186