Screen and analysis of key disease genes for precancerous lesions of oral buccal mucosa induced by DMBA in golden hamsters

Chen,Dan; Yang,Kai; Zhang,Guodong; Mei,Jie; Xiang,Li

doi:10.3892/ol.2010.228

Screen and analysis of key disease genes for precancerous lesions of oral buccal mucosa induced by DMBA in golden hamsters

Authors:
- Dan Chen
- Kai Yang
- Guodong Zhang
- Jie Mei
- Li Xiang
View Affiliations

Affiliations: Department of Oral and Maxillofacial Surgery, the First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, P.R. China
Published online on: December 23, 2010 https://doi.org/10.3892/ol.2010.228
Pages: 265-271

Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )

Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )

Cited By (CrossRef): 0 citations Loading Articles...

Abstract

7,12-Dimethylbenz(a)-anthracene (DMBA)-induced oral buccal mucosa squamous cell carcinoma in Syrian golden hamsters was used to establish precancerous lesions. Agilent rat whole-genome microarray and biological information analysis were used to screen for genes related to key diseases during the transformation of normal buccal mucosa to precancerous lesions in golden hamsters. DMBA acetone solution (0.5%) was used to establish a model of precancerous lesions in oral buccal mucosa in golden hamsters. The results showed that a total of 1331 genes were differentially expressed, including 1278 known, 53 unknown, 747 up-regulated and 584 down-regulated genes. Analysis revealed a total of 14 gene interaction pathways that significantly associated with the 1278 known differentially expressed genes (P<0.05). In conclusion, the occurrence of precancerous lesions in the oral buccal mucosa of golden hamsters was caused by a number of genetic changes that resulted in changes to their respective pathways. Key candidate genes for the formation of precancerous lesions in oral buccal mucosa included Cyp2b13, Orc1L, casp8, CCL5, CXCL12, CCL20, Serping1, P518/Qrfp, F5, TFPI, Vcam1, Fn1, Angpt2, Lcp2, Cxadr, Lyn, Hck, Btk, RGD1564385/fes, Vav1 and IL5ra.

View Figures

View References

1	Kademani D, Bell RB, Schmidt BL, Blanchaert R, Fernandes R, Lambert P and Tucker WM: Oral and maxillofacial surgeons treating oral cancer: a preliminary report from the American Association of Oral and Maxillofacial Surgeons Task Force on Oral Cancer. J Oral Maxillofac Surg. 66:2151–2157. 2008. View Article : Google Scholar : PubMed/NCBI
2	Bernier J and Cooper JS: Chemoradiation after surgery for high-risk head and neck cancer patients: how strong is the evidence? Oncologist. 10:215–224. 2005. View Article : Google Scholar : PubMed/NCBI
3	Carinci F, Lo Muzio L, Piattelli A, et al: Genetic portrait of mild and severe lingual dysplasia. Oral Oncol. 41:365–374. 2005. View Article : Google Scholar : PubMed/NCBI
4	Choi P and Chen C: Genetic expression profiles and biologic pathway alterations in head and neck squamous cell carcinoma. Cancer. 104:1113–1128. 2005. View Article : Google Scholar : PubMed/NCBI
5	Shiu MN and Chen TH: Impact of betel quid, tobacco and alcohol on three-stage disease natural history of oral leukoplakia and cancer: implication for prevention of oral cancer. Eur J Cancer Prev. 13:39–45. 2004. View Article : Google Scholar : PubMed/NCBI
6	Nagata M, Fujita H, Ida H, et al: Identification of potential biomarkers of lymph node metastasis in oral squamous cell carcinoma by cDNA microarray analysis. Int J Cancer. 106:683–689. 2003. View Article : Google Scholar : PubMed/NCBI
7	Kashiwazaki H, Hassan NM, Hamada J, et al: Gene expression profile changes correlated with lymph node metastasis in oral squamous cell carcinoma. Odontology. 96:38–43. 2008. View Article : Google Scholar : PubMed/NCBI
8	Arora S, Matta A, Shukla NK, Deo SV and Ralhan R: Identification of differentially expressed genes in oral squamous cell carcinoma. Mol Carcinog. 42:97–108. 2005. View Article : Google Scholar : PubMed/NCBI
9	Erdem NF, Carlson ER and Gerard DA: Characterization of gene expression profiles of 3 different human oral squamous cell carcinoma cell lines with different invasion and metastatic capacities. J Oral Maxillofac Surg. 66:918–927. 2008. View Article : Google Scholar : PubMed/NCBI
10	Mendez E, Cheng C, Farwell DG, et al: Transcriptional expression profiles of oral squamous cell carcinomas. Cancer. 95:1482–1494. 2002. View Article : Google Scholar : PubMed/NCBI
11	Gottschlich S, Ambrosch P, Cordes C, Gorogh T, Schreiber S and Hasler R: Gene expression profiling of head and neck squamous cell carcinoma using cDNA microarrays. Int J Oncol. 29:605–613. 2006.PubMed/NCBI
12	Estilo CL, O-charoenrat P, Talbot S, et al: Oral tongue cancer gene expression profiling: identification of novel potential prognosticators by oligonucleotide microarray analysis. BMC Cancer. 9:112009. View Article : Google Scholar
13	Salley JJ: Experimental carcinogenesis in the cheek pouch of the Syrian hamster. J Dent Res. 33:253–262. 1954. View Article : Google Scholar : PubMed/NCBI
14	Feng L and Wang Z: Chemopreventive effect of celecoxib in oral precancers and cancers. Laryngoscope. 116:1842–1845. 2006. View Article : Google Scholar : PubMed/NCBI
15	Gimenez-Conti IB and Slaga TJ: The hamster cheek pouch carcinogenesis model. J Cell Biochem Suppl. 17F:83–90. 1993. View Article : Google Scholar : PubMed/NCBI
16	World Health Organization. Report of a meeting of investigators on the histological definition of precancerous lesions. Geneva: World Health Organization; pp. 7311973
17	Stupack DG, Teitz T, Potter MD, et al: Potentiation of neuroblastoma metastasis by loss of caspase-8. Nature. 439:95–99. 2006. View Article : Google Scholar : PubMed/NCBI
18	McKee AE and Thiele CJ: Targeting caspase 8 to reduce the formation of metastases in neuroblastoma. Expert Opin Ther Targets. 10:703–708. 2006. View Article : Google Scholar : PubMed/NCBI
19	DePamphilis ML: Cell cycle dependent regulation of the origin recognition complex. Cell Cycle. 4:70–79. 2005. View Article : Google Scholar : PubMed/NCBI
20	Tatsumi Y, Ohta S, Kimura H, Tsurimoto T and Obuse C: The ORC1 cycle in human cells: I. cell cycle-regulated oscillation of human ORC1. J Biol Chem. 278:41528–41534. 2003. View Article : Google Scholar : PubMed/NCBI
21	Burger JA and Kipps TJ: CXCR4: a key receptor in the crosstalk between tumor cells and their microenvironment. Blood. 107:1761–1767. 2006. View Article : Google Scholar : PubMed/NCBI
22	Vaday GG, Peehl DM, Kadam PA and Lawrence DM: Expression of CCL5 (RANTES) and CCR5 in prostate cancer. Prostate. 66:124–134. 2006. View Article : Google Scholar : PubMed/NCBI
23	Fushimi T, Kojima A, Moore MA and Crystal RG: Macrophage inflammatory protein 3alpha transgene attracts dendritic cells to established murine tumors and suppresses tumor growth. J Clin Invest. 105:1383–1393. 2000. View Article : Google Scholar
24	Rubie C, Frick VO, Wagner M, et al: Chemokine expression in hepatocellular carcinoma versus colorectal liver metastases. World J Gastroenterol. 12:6627–6633. 2006.PubMed/NCBI
25	Yu JL, May L, Lhotak V, et al: Oncogenic events regulate tissue factor expression in colorectal cancer cells: implications for tumor progression and angiogenesis. Blood. 105:1734–1741. 2005. View Article : Google Scholar : PubMed/NCBI
26	Nakao S, Kuwano T, Ishibashi T, Kuwano M and Ono M: Synergistic effect of TNF-alpha in soluble VCAM-1-induced angiogenesis through alpha 4 integrins. J Immunol. 170:5704–5711. 2003. View Article : Google Scholar : PubMed/NCBI
27	Gubala E, Wiench M, Oczko-Wojciechowska M, et al: [Gene expression analysis by DNA microarray in papillary thyroid cancer]. Endokrynol Pol. 56:752–757. 2005.
28	Koga K, Todaka T, Morioka M, et al: Expression of angiopoietin- 2 in human glioma cells and its role for angiogenesis. Cancer Res. 61:6248–6254. 2001.PubMed/NCBI
29	Zhang LL, He DL, Li X, Li L, Zhu GD, Zhang D and Wang XY: Overexpression of coxsackie and adenovirus receptor inhibit growth of human bladder cancer cell in vitro and in vivo. Acta Pharmacol Sin. 28:895–900. 2007. View Article : Google Scholar : PubMed/NCBI
30	Cao Y and Prescott SM: Many actions of cyclooxygenase-2 in cellular dynamics and in cancer. J Cell Physiol. 190:279–286. 2002. View Article : Google Scholar : PubMed/NCBI
31	Poff CD and Balazy M: Drugs that target lipoxygenases and leukotrienes as emerging therapies for asthma and cancer. Curr Drug Targets Inflamm Allergy. 3:19–33. 2004. View Article : Google Scholar : PubMed/NCBI
32	Brookman-Amissah N, Mackay AG and Swann PF: Isolation and sequencing of the cDNA of a novel cytochrome P450 from rat oesophagus. Carcinogenesis. 22:155–160. 2001. View Article : Google Scholar : PubMed/NCBI
33	Konecny GE, Pegram MD, Venkatesan N, et al: Activity of the dual kinase inhibitor lapatinib (GW572016) against HER-2-overexpressing and trastuzumab-treated breast cancer cells. Cancer Res. 66:1630–1639. 2006. View Article : Google Scholar : PubMed/NCBI
34	Suh HS, Kim MO and Lee SC: Inhibition of granulocyte-macrophage colony-stimulating factor signaling and microglial proliferation by anti-CD45RO: role of Hck tyrosine kinase and phosphatidylinositol 3-kinase/Akt. J Immunol. 174:2712–2719. 2005. View Article : Google Scholar : PubMed/NCBI
35	Grosso S, Puissant A, Dufies M, et al: Gene expression profiling of imatinib and PD166326-resistant CML cell lines identifies Fyn as a gene associated with resistance to BCR-ABL inhibitors. Mol Cancer Ther. 8:1924–1933. 2009. View Article : Google Scholar : PubMed/NCBI
36	Stephens LR, Anderson KE and Hawkins PT: Src family kinases mediate receptor-stimulated, phosphoinositide 3-kinase-dependent, tyrosine phosphorylation of dual adaptor for phosphotyrosine and 3-phosphoinositides-1 in endothelial and B cell lines. J Biol Chem. 276:42767–42773. 2001. View Article : Google Scholar