Identification of responders/non-responders to 5-fluorouracil based on individual 50% inhibitory area under the concentration curve of 5-fluorouracil obtained with collagen gel droplet‑embedded culture-drug sensitivity test in colorectal cancer

  • Authors:
    • Takumi Ochiai
    • Kazuhiko Nishimura
    • Tomoo Watanabe
    • Masayuki Kitajima
    • Akinori Nakatani
    • Takashi Marusasa
    • Tadasuke Hashiguchi
    • Takayuki Uchida
    • Naoki Sakuyama
    • Tsuyoshi Sato
    • Kennji Kishine
    • Shunji Futagawa
    • Isao Nagaoka
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  • Published online on: January 21, 2011     https://doi.org/10.3892/ol.2011.251
  • Pages: 309-313
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Abstract

We previously reported the 5-fluorouracil (5-FU) sensitivity of cancer cells obtained from colorectal cancer (CRC) patients using the collagen gel droplet-embedded culture-drug sensitivity test (CD-DST). Multiple drug concentrations and contact durations, and the area under the concentration curve (AUC) and growth inhibition rate (IR) were combined, resulting in the AUC-IR curve, which was approximated to the logarithmic curve. Moreover, the individualized AUCIR50, the AUC value which gives 50% growth inhibition, was calculated using the AUC-IR curve. This study aimed to identify responders/non-responders to 5-FU based on the individual AUCIR50 obtained with CD-DST in order to establish individualized chemotherapy for CRC patients. The individual AUCIR50 was calculated from each AUC-inhibition rate regression curve in all patients using the CD-DST. The cumulative distribution of the individual AUCIR50 in CRC patients was evaluated. The cumulative distribution of the individual AUCIR50 was regressed over the sigmoid curve (logarithmic scale). The approximate expression was almost exactly y=ab^exp(-cx) (a=0.9739, b=1.7096E-21, c=0.8990, the sum of square residuals, 0.0279). In the 80 cases examined, no notable change was observed in the regression curve when the number of patients increased. A standard curve was obtained describing responders to 5-FU among all CRC patients. From this standard curve, we ascertained that non-responders accounted for approximately 5% of all patients. Moreover, we were able to classify responders into good or intermediate responders to 5-FU. The standard curve describing response to 5-FU in CRC patients offers a useful tool in the establishment of individualized chemotherapy.
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March-April 2011
Volume 2 Issue 2

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Spandidos Publications style
Ochiai T, Nishimura K, Watanabe T, Kitajima M, Nakatani A, Marusasa T, Hashiguchi T, Uchida T, Sakuyama N, Sato T, Sato T, et al: Identification of responders/non-responders to 5-fluorouracil based on individual 50% inhibitory area under the concentration curve of 5-fluorouracil obtained with collagen gel droplet‑embedded culture-drug sensitivity test in colorectal cancer. Oncol Lett 2: 309-313, 2011
APA
Ochiai, T., Nishimura, K., Watanabe, T., Kitajima, M., Nakatani, A., Marusasa, T. ... Nagaoka, I. (2011). Identification of responders/non-responders to 5-fluorouracil based on individual 50% inhibitory area under the concentration curve of 5-fluorouracil obtained with collagen gel droplet‑embedded culture-drug sensitivity test in colorectal cancer. Oncology Letters, 2, 309-313. https://doi.org/10.3892/ol.2011.251
MLA
Ochiai, T., Nishimura, K., Watanabe, T., Kitajima, M., Nakatani, A., Marusasa, T., Hashiguchi, T., Uchida, T., Sakuyama, N., Sato, T., Kishine, K., Futagawa, S., Nagaoka, I."Identification of responders/non-responders to 5-fluorouracil based on individual 50% inhibitory area under the concentration curve of 5-fluorouracil obtained with collagen gel droplet‑embedded culture-drug sensitivity test in colorectal cancer". Oncology Letters 2.2 (2011): 309-313.
Chicago
Ochiai, T., Nishimura, K., Watanabe, T., Kitajima, M., Nakatani, A., Marusasa, T., Hashiguchi, T., Uchida, T., Sakuyama, N., Sato, T., Kishine, K., Futagawa, S., Nagaoka, I."Identification of responders/non-responders to 5-fluorouracil based on individual 50% inhibitory area under the concentration curve of 5-fluorouracil obtained with collagen gel droplet‑embedded culture-drug sensitivity test in colorectal cancer". Oncology Letters 2, no. 2 (2011): 309-313. https://doi.org/10.3892/ol.2011.251