Acquisition of selective antitumoral effects of recombinant adeno-associated virus by genetically inserting tumor-targeting peptides into capsid proteins

  • Authors:
    • Han Saem Lee
    • Ji Yun Kim
    • Won Il Lee
    • Sung Jin Kim
    • Min Ji Ko
    • Sunjoo Jeong
    • Keerang Park
    • Han Choe
    • Heuiran Lee
  • View Affiliations

  • Published online on: August 8, 2011     https://doi.org/10.3892/ol.2011.376
  • Pages: 1113-1119
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Abstract

Recombinant adeno‑associated virus serotype 5 (rAAV5) is considered to be a promising gene transfer vehicle. However, preferential gene delivery to the tumor remains a requirement for cancer treatment. We generated rAAV5 mutants bearing tumor marker-binding peptides and analyzed their properties as viral vectors, as well as their transduction efficiencies and preferential antitumoral potencies. All of the mutants were successfully produced. Transduction analyses showed that rAAV5 mutants harboring tumor-homing peptides, including RGD and TnC, transduced human cancer cells expressing corresponding receptors on their surfaces. RGDS peptides and TnC antibodies significantly suppressed trans-duction by rAAV5-RGD and rAAV5-TnC. Cytotoxicity was evident upon transfer of HSV-TK to cells by re-targeted rAAV5. These results provide evidence that rAAV5 vectors, genetically armed with tumor-targeting ligands, preferentially infect human cancer cells harboring the corresponding receptors, thereby inducing antitumoral effects. Further optimization of rAAV5 mutant viruses should thus facilitate practical exploitation of these vectors for gene-based cancer treatment.
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November-December 2011
Volume 2 Issue 6

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Lee H, Kim J, Lee W, Kim S, Ko M, Jeong S, Park K, Choe H and Lee H: Acquisition of selective antitumoral effects of recombinant adeno-associated virus by genetically inserting tumor-targeting peptides into capsid proteins. Oncol Lett 2: 1113-1119, 2011
APA
Lee, H., Kim, J., Lee, W., Kim, S., Ko, M., Jeong, S. ... Lee, H. (2011). Acquisition of selective antitumoral effects of recombinant adeno-associated virus by genetically inserting tumor-targeting peptides into capsid proteins. Oncology Letters, 2, 1113-1119. https://doi.org/10.3892/ol.2011.376
MLA
Lee, H., Kim, J., Lee, W., Kim, S., Ko, M., Jeong, S., Park, K., Choe, H., Lee, H."Acquisition of selective antitumoral effects of recombinant adeno-associated virus by genetically inserting tumor-targeting peptides into capsid proteins". Oncology Letters 2.6 (2011): 1113-1119.
Chicago
Lee, H., Kim, J., Lee, W., Kim, S., Ko, M., Jeong, S., Park, K., Choe, H., Lee, H."Acquisition of selective antitumoral effects of recombinant adeno-associated virus by genetically inserting tumor-targeting peptides into capsid proteins". Oncology Letters 2, no. 6 (2011): 1113-1119. https://doi.org/10.3892/ol.2011.376