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Article

Different expression of alternative lengthening of telomere (ALT)-associated proteins/mRNAs in osteosarcoma cell lines

  • Authors:
    • Yi Zhang
    • Lin Cai
    • Ren-Xiong Wei
    • Hao Hu
    • Wei Jin
    • Xiao-Bin Zhu
  • View Affiliations / Copyright

    Affiliations: Department of Orthopedics, Zhongnan Hospital, Wuhan University, Wuhan, Hubei 430071, P.R. China
  • Pages: 1327-1332
    |
    Published online on: September 2, 2011
       https://doi.org/10.3892/ol.2011.403
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Abstract

Tumors, including osteosarcoma (OS), are capable of evading senescence and cell death, which is caused by telomere loss with cell division. Alternative lengthening of telomeres (ALT) is considered as the main telomere maintenance mechanism in OS. In this study, we investigated the expression of ALT-associated proteins and mRNAs in human OS cell lines. Western blotting was used to detect the protein expression in OS cell lines, while the expression of mRNA was determined by reverse‑transcriptase PCR and quantitative real-time PCR analysis. Whole-genome expression arrays were used to analyze the expression of all the mRNAs involved in telomere maintenance mechanisms (TMMs) including human telomerase reverse transcriptase, promyelocytic leukemia proteins and other related proteins. OS and normal cell lines do not express telomerase reverse transcriptase (hTERT) as a key subunit of telomerase, although they show varying levels of ALT-associated proteins and mRNAs such as PML, Rad52, MRE11 and FEN1 by Western blotting and quantitative real-time PCR analysis. A number of mRNAs that play essential roles in ALT are expressed more in OS cell lines than in the osteoblast cell line, as shown by whole-genome expression arrays. In conclusion, OS cell lines maintain their telomere length primarily through the ALT mechanism. There are numerous other proteins that regulate this process in OS; therefore, anti‑ALT therapy may be a more effective method to treat OS than anti-telomerase therapy.
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Copy and paste a formatted citation
Spandidos Publications style
Zhang Y, Cai L, Wei R, Hu H, Jin W and Zhu X: Different expression of alternative lengthening of telomere (ALT)-associated proteins/mRNAs in osteosarcoma cell lines. Oncol Lett 2: 1327-1332, 2011.
APA
Zhang, Y., Cai, L., Wei, R., Hu, H., Jin, W., & Zhu, X. (2011). Different expression of alternative lengthening of telomere (ALT)-associated proteins/mRNAs in osteosarcoma cell lines. Oncology Letters, 2, 1327-1332. https://doi.org/10.3892/ol.2011.403
MLA
Zhang, Y., Cai, L., Wei, R., Hu, H., Jin, W., Zhu, X."Different expression of alternative lengthening of telomere (ALT)-associated proteins/mRNAs in osteosarcoma cell lines". Oncology Letters 2.6 (2011): 1327-1332.
Chicago
Zhang, Y., Cai, L., Wei, R., Hu, H., Jin, W., Zhu, X."Different expression of alternative lengthening of telomere (ALT)-associated proteins/mRNAs in osteosarcoma cell lines". Oncology Letters 2, no. 6 (2011): 1327-1332. https://doi.org/10.3892/ol.2011.403
Copy and paste a formatted citation
x
Spandidos Publications style
Zhang Y, Cai L, Wei R, Hu H, Jin W and Zhu X: Different expression of alternative lengthening of telomere (ALT)-associated proteins/mRNAs in osteosarcoma cell lines. Oncol Lett 2: 1327-1332, 2011.
APA
Zhang, Y., Cai, L., Wei, R., Hu, H., Jin, W., & Zhu, X. (2011). Different expression of alternative lengthening of telomere (ALT)-associated proteins/mRNAs in osteosarcoma cell lines. Oncology Letters, 2, 1327-1332. https://doi.org/10.3892/ol.2011.403
MLA
Zhang, Y., Cai, L., Wei, R., Hu, H., Jin, W., Zhu, X."Different expression of alternative lengthening of telomere (ALT)-associated proteins/mRNAs in osteosarcoma cell lines". Oncology Letters 2.6 (2011): 1327-1332.
Chicago
Zhang, Y., Cai, L., Wei, R., Hu, H., Jin, W., Zhu, X."Different expression of alternative lengthening of telomere (ALT)-associated proteins/mRNAs in osteosarcoma cell lines". Oncology Letters 2, no. 6 (2011): 1327-1332. https://doi.org/10.3892/ol.2011.403
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