Sensitization of breast cancer cells to taxol by inhibition of taxol resistance gene 1

  • Authors:
    • Zhigang Bai
    • Zhongtao Zhang
    • Xiang Qu
    • Wei Han
    • Xuemei Ma
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  • Published online on: September 9, 2011     https://doi.org/10.3892/ol.2011.416
  • Pages: 135-140
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Abstract

The purpose of this study was to determine the role of taxol resistance gene 1 (TXR1) in a taxol-resistant breast cancer cell line. Expression levels of TXR1 and thrombospondin-1 (TSP1) were detected by RT-PCR and Western blot analysis. In MCF-7 cells transfected with TXR1 plasmids, the expression of a number of drug resistance genes was monitored, as well as cell proliferation. MCF-7 cells were also transiently transfected with a chemically synthesized small interfering RNA (siRNA) targeting TXR1. Taxol concentrations varying from 0.6 to 6 µg/ml were shown to inhibit the proliferation of MCF-7 cells in a time- and dose-dependent manner by arresting cells in the G2/M phase. Additionally, 0.06 µg/ml taxol was used to establish a taxol-resistant MCF-7 cell line, MCF-7/T. When TXR1 was exogenously expressed, a taxol-resistant phenotype was further induced and the expression levels of BCRP, GSTP1 and MVP increased. Transient transfection of TXR1-targeting siRNAs was shown to restore the chemosensitivity of MCF-7 cells. These results suggest that an increased expression of TXR1 is a novel mechanism for reversing the taxol resistance of breast cancer cells.
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January 2012
Volume 3 Issue 1

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Bai Z, Zhang Z, Qu X, Han W and Ma X: Sensitization of breast cancer cells to taxol by inhibition of taxol resistance gene 1. Oncol Lett 3: 135-140, 2012.
APA
Bai, Z., Zhang, Z., Qu, X., Han, W., & Ma, X. (2012). Sensitization of breast cancer cells to taxol by inhibition of taxol resistance gene 1. Oncology Letters, 3, 135-140. https://doi.org/10.3892/ol.2011.416
MLA
Bai, Z., Zhang, Z., Qu, X., Han, W., Ma, X."Sensitization of breast cancer cells to taxol by inhibition of taxol resistance gene 1". Oncology Letters 3.1 (2012): 135-140.
Chicago
Bai, Z., Zhang, Z., Qu, X., Han, W., Ma, X."Sensitization of breast cancer cells to taxol by inhibition of taxol resistance gene 1". Oncology Letters 3, no. 1 (2012): 135-140. https://doi.org/10.3892/ol.2011.416