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Article

Copy number changes of CRISP3 in oral squamous cell carcinoma

  • Authors:
    • Wen-Chang Ko
    • Keisuke Sugahara
    • Takumi Sakuma
    • Ching-Yu Yen
    • Shyun-Yeu Liu
    • Gwo-An Liaw
    • Takahiko Shibahara
  • View Affiliations / Copyright

    Affiliations: Department of Oral and Maxillofacial Surgery, Tokyo Dental College, Mihama, Chiba 261-8502, Japan, Department of Oral and Maxillofacial Surgery, Chi-Mei Medical Center, Yongkang, Taiwan, R.O.C.
  • Pages: 75-81
    |
    Published online on: September 9, 2011
       https://doi.org/10.3892/ol.2011.418
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Abstract

The aim of this study was to identify tumor suppressor genes (TSGs) in oral squamous cell carcinoma (OSCC) using whole-genome analysis of microarray technology and real-time quantitative polymerase chain reaction (QPCR). We applied whole-genome analysis of TSGs in the specimens from 3 patients of OSCC by microarray technology. A total of 11 genes, CRISP3, SCGB3A1, AGR2, PIP, C20orf114, TFF1, STATH, AZGP1, MUC7, DMBT1 and LOC389429, were found to be down-regulated, and 2, matrix metallopeptidase (MMP) 1 and MMP3, were found to be up-regulated in the 3 OSCC patients using microarray technology. In this study, we selected the CRISP3 gene. CRISP3 belongs to the cystein-rich secretary protein gene family in chromosome 6p12.3. CRISP3 has been found in the salivary gland, spleen and prostate gland and is a prominent biomarker in the gene expression of prostate cancer. Down-regulation of this gene was previously observed in OSCC. No studies examining the DNA copy number of CRISP3 in detail exist. We analyzed the DNA copy number of CRISP3 in 5 OSCC-derived cell lines (SAS, Ca9-22, KON, HSC2 and HSC4) and 60 OSCC tissues by real-time QPCR. The DNA copy number loss of CRISP3 was observed in 2 of the 5 OSCC-derived cell lines (SAS, HSC2) and in 24 of 60 patients (40.0%) using real-time QPCR. A significant statistical correlation between the copy number loss and gender and T classification was observed. These results indicate that the inactivation of CRISP3 is an early event in OSCC, since the T1/T2 classification is correlated with DNA copy number loss of CRISP3, whereas T3/T4 classification is not. We conclude that CRISP3 may be involved in the carcinogenesis of OSCC.
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Copy and paste a formatted citation
Spandidos Publications style
Ko W, Sugahara K, Sakuma T, Yen C, Liu S, Liaw G and Shibahara T: Copy number changes of CRISP3 in oral squamous cell carcinoma. Oncol Lett 3: 75-81, 2012.
APA
Ko, W., Sugahara, K., Sakuma, T., Yen, C., Liu, S., Liaw, G., & Shibahara, T. (2012). Copy number changes of CRISP3 in oral squamous cell carcinoma. Oncology Letters, 3, 75-81. https://doi.org/10.3892/ol.2011.418
MLA
Ko, W., Sugahara, K., Sakuma, T., Yen, C., Liu, S., Liaw, G., Shibahara, T."Copy number changes of CRISP3 in oral squamous cell carcinoma". Oncology Letters 3.1 (2012): 75-81.
Chicago
Ko, W., Sugahara, K., Sakuma, T., Yen, C., Liu, S., Liaw, G., Shibahara, T."Copy number changes of CRISP3 in oral squamous cell carcinoma". Oncology Letters 3, no. 1 (2012): 75-81. https://doi.org/10.3892/ol.2011.418
Copy and paste a formatted citation
x
Spandidos Publications style
Ko W, Sugahara K, Sakuma T, Yen C, Liu S, Liaw G and Shibahara T: Copy number changes of CRISP3 in oral squamous cell carcinoma. Oncol Lett 3: 75-81, 2012.
APA
Ko, W., Sugahara, K., Sakuma, T., Yen, C., Liu, S., Liaw, G., & Shibahara, T. (2012). Copy number changes of CRISP3 in oral squamous cell carcinoma. Oncology Letters, 3, 75-81. https://doi.org/10.3892/ol.2011.418
MLA
Ko, W., Sugahara, K., Sakuma, T., Yen, C., Liu, S., Liaw, G., Shibahara, T."Copy number changes of CRISP3 in oral squamous cell carcinoma". Oncology Letters 3.1 (2012): 75-81.
Chicago
Ko, W., Sugahara, K., Sakuma, T., Yen, C., Liu, S., Liaw, G., Shibahara, T."Copy number changes of CRISP3 in oral squamous cell carcinoma". Oncology Letters 3, no. 1 (2012): 75-81. https://doi.org/10.3892/ol.2011.418
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