Transforming growth factor β-induced epithelial-mesenchymal transition increases cancer stem-like cells in the PANC-1 cell line

Wang,Hui; Wu,Junli; Zhang,Ye; Xue,Xiaofeng; Tang,Dong; Yuan,Zhongxu; Chen,Minyong; Wei,Jishu; Zhang,Jingjing; Miao,Yi

doi:10.3892/ol.2011.448

Transforming growth factor β-induced epithelial-mesenchymal transition increases cancer stem-like cells in the PANC-1 cell line

Authors:
- Hui Wang
- Junli Wu
- Ye Zhang
- Xiaofeng Xue
- Dong Tang
- Zhongxu Yuan
- Minyong Chen
- Jishu Wei
- Jingjing Zhang
- Yi Miao
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Affiliations: Laboratory of General Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, P.R. China, Department of General Surgery, The First Affiliated Hospital of Nanjing Medical University, Nanjing 210029, P.R. China, Jiangsu Province Academy of Clinical Medicine Institute of Tumor Biology, Nanjing 210029, P.R. China
Published online on: October 19, 2011 https://doi.org/10.3892/ol.2011.448
Pages: 229-233

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Abstract

The epithelial-mesenchymal transition plays a crucial role in the progression of pancreatic cancer. The aim of this study was to examine the possible association between the epithelial-mesenchymal transition and cancer stem-like cells in pancreatic cancer. We used transforming growth factor β to induce an epithelial-mesenchymal transition. The proportion of pancreatic cancer stem-like cells was measured and sorted by flow cytometry. The expression of markers was measured by quantitative PCR and Western blot analysis. Cell cycle distribution was assessed by flow cytometry. We evaluated the migration and invasion activity by Transwell tests. The proportion of pancreatic cancer stem‑like cells was significantly increased following transforming growth factor β treatment. Cells were sorted in culture, the cancer stem-like cells exhibited a higher degree of epithelial-mesenchymal transition and demonstrated upregulation of vimentin, a mesenchymal phenotypic marker, compared to the CD44-CD24- cells. Pancreatic cancer stem-like cells exhibited greater invasion and migration activity in vitro compared to the CD44-CD24- cells. These results suggested a direct link between epithelial-mesenchymal transition and cancer stem‑like cells in pancreatic cancer.

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