Nucleolar organizer regions in a chronic stress and oral cancer model

Muñoz Ruz,Ivonne   Andrea; Droguett Ossa,Daniel   Andrés; Donoso Torres,Wendy   Karina; Kemmerling,Ulrike; Venegas Rojas,Bernardo   Arturo; Rivera Martínez,César   Andrés

doi:10.3892/ol.2011.514

Nucleolar organizer regions in a chronic stress and oral cancer model

Authors:
- Ivonne Andrea Muñoz Ruz
- Daniel Andrés Droguett Ossa
- Wendy Karina Donoso Torres
- Ulrike Kemmerling
- Bernardo Arturo Venegas Rojas
- César Andrés Rivera Martínez
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Affiliations: Department of Stomatology, Faculty of Health Sciences, University of Talca, Talca, Chile, Department of Basic Biomedical Sciences, Faculty of Health Sciences, University of Talca, Talca, Chile
Published online on: December 6, 2011 https://doi.org/10.3892/ol.2011.514
Pages: 541-544

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Abstract

This study aimed to examine the role of chronic restraint stress (RS) on oral squamous cell carcinomas induced by 4-nitroquinoline-1-oxide (4-NQO) in CF-1 mouse tongues, measured by the expression of argyrophilic staining of nucleolar organizer regions (AgNOR). Thirty one samples of lingual epithelial tissue of CF-1 mice with a diagnosis of oral squamous cell carcinoma (OSSC) were assigned to two experimental groups: the RS/4-NQO group, where animals received RS and induction of oral chemical carcinogenesis (n=17); and the 4-NQO group, where animals received induction of chemical carcinogenesis without restraint stress (n=14). The mean number and distribution pattern of AgNOR were recorded. The mean AgNOR number per cell was found to be slightly higher in the 4-NQO group. AgNOR in the RS/4-NQO group revealed a higher tendency to be arranged in a clumped distribution compared to the 4-NQO group. No statistically significant difference was found between the groups. In conclusion, the induction of chronic restraint stress in CF-1 mice does not increase the number or affect the distribution pattern of AgNORs in OSSC induced by 4-NQO.

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1	Saul A, Oberyszyn T, Daugherty C, Kusewitt D, Jones S, Jewell S, et al: Chronic stress and susceptibility to skin cancer. J Natl Cancer Inst. 97:1760–1767. 2005. View Article : Google Scholar : PubMed/NCBI
2	Thaker P, Han L, Kamat A, Arevalo J, Takahashi R, Lu C, et al: Chronic stress promotes tumor growth and angiogenesis in a mouse model of ovarian carcinoma. Nat Med. 12:939–944. 2006. View Article : Google Scholar : PubMed/NCBI
3	Rivera C, Droguett D, Kemmerling U and Venegas B: Chronic restraint stress in oral squamous cell carcinoma. J Dent Res. 90:799–803. 2011. View Article : Google Scholar : PubMed/NCBI
4	Cano L, Álvarez G, Valencia W, Ramírez J and Prada C: Análisis del marcador tisular AgNOR en leucoplasia y carcinoma escamocelular oral. Medicina Oral. 7:17–25. 2002.
5	Kanitakis J, Hoyo E, Hermier C, Chouvet B and Thivolet J: Nucleolar organizer region enumeration in keratoacanthomas and squamous cell carcinomas of the skin. Cancer. 69:2937–2941. 1992. View Article : Google Scholar : PubMed/NCBI
6	Schwint A, Gomez E, Itoiz M and Cabrini R: Nucleolar organizer regions as markers of incipient cellular alterations in squamous epithelium. J Dent Res. 72:1233–1236. 1993. View Article : Google Scholar : PubMed/NCBI
7	Korneyev I, Mamaev N, Kozlov V and Rybakova M: Interphase argyrophilic nucleolar organizer regions and nucleolar counts in transitional cell bladder tumors. Mol Pathol. 53:129–132. 2000. View Article : Google Scholar : PubMed/NCBI
8	Palomo-González M, Pérez-Requena J, Rego-González M, López-Nieto M and Cañizares C: Evaluación de regiones organizadoras nucleolares argirófilas (AgNORs) en neoplasias foliculares tiroideas. Rev Esp Patol. 35:95–100. 2002.PubMed/NCBI
9	Pich A, Chiusa L and Navone R: Prognostic relevance of cell proliferation in head and neck tumors. Ann Oncol. 15:1319–1329. 2004. View Article : Google Scholar : PubMed/NCBI
10	Cabrini R, Schwint A, Mendez A, Femopase F, Lanfranchi H and Itoiz M: Morphometric study of nucleolar organizer regions inchuman oral normal mucosa, papilloma and squamous cell carcinoma. Oral Pathol Med. 21:275–279. 1992. View Article : Google Scholar : PubMed/NCBI
11	Derenzini M: The AgNORs. Micron. 31:117–120. 2000. View Article : Google Scholar
12	Leyva Huerta E, Vega E, Ramìrez M and Zárate A: Marcador de regiones de organizadores nucleolares en lesiones epiteliales de cavidad bucal. Rev Esp Patol. 37:287–293. 2004.
13	López-Muñiz A, Triviño A, Hernández L and Herrero A: Valoración de las regiones organizadoras nucleares en lesiones melánicas benignas y malignas de piel. Rev Cubana Med. 40:17–23. 2001.PubMed/NCBI
14	Wozniak Z, Usson Y, Parazza F, Champelovier P, Leroux D and Seigneurin D: Quantitative analysis of three-dimensional distribution of AgNOR proteins during interphase in leukemic cells. Cytometry. 24:14–26. 1996. View Article : Google Scholar : PubMed/NCBI
15	Ploton D, Menager M, Jeanneson P, Himber G, Piegon F and Adent JJ: Improvement in the staining and in the visualization of argyrophilic proteins of the nucleolar organizer region at the optical level. Histochem J. 18:5–14. 1986. View Article : Google Scholar : PubMed/NCBI
16	Warnakulasuriya K and Johnson N: Nucleolar organiser region (NOR) distribution as a diagnostic marker in oral keratosis, dysplasia and squamous cell carcinoma. J Oral Pathol Med. 22:77–81. 1993. View Article : Google Scholar : PubMed/NCBI
17	Lindner L: Improvements in the silver-staining technique for nucleolar organizer regions (AgNOR). J Histochem Cytochem. 41:439–445. 1993. View Article : Google Scholar : PubMed/NCBI
18	Venegas B, Vildósola M and Droguett D: AgNORs expression in experimental carcinogenesis. J Dent Res. 89(Spec Iss B): pp. 1916(www.dentalresearch.orgurisimplewww.dentalresearch.org). 2010
19	Rocher E, Blanco A and Palaoro L: Utilidad de la técnica de AgNOR en la interpretación de los derrames de cavidades serosas. Rev Med Chil. 128:963–968. 2000.