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March 2012 Volume 3 Issue 3

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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

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Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

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Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

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Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

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Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

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Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

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International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

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International Journal of Epigenetics

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Article

Cancer stem-like cells persist in established cell lines through autocrine activation of EGFR signaling

  • Authors:
    • Jeongyub Kim
    • Jieun Jung
    • Su-Jae Lee
    • Jae-Seon Lee
    • Myung-Jin Park
  • View Affiliations / Copyright

    Affiliations: Divisions of Radiation Cancer Research, Korea Institute of Radiological and Medical Sciences, Nowon-gu, Seoul 139-706, Republic of Korea, Department of Chemistry and Research Institute for Natural Sciences, Hanyang University, Seongdong-gu, Seoul 133-791, Republic of Korea
  • Pages: 607-612
    |
    Published online on: December 21, 2011
       https://doi.org/10.3892/ol.2011.531
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Abstract

Recent studies have shown that a small subpopulation of stem-like cancer cells within most solid tumors are responsible for the malignancy of aggressive cancer cells, including tumorigenicity and relapse of solid tumors. These tumor cells may be enriched and maintained in vitro in the presence of growth factors (GFs), including epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF), without serum in almost all solid tumor cell lines. In the present study, however, we found that the enrichment of cancer stem-like cells (CSCs) from established cell lines using sphere cultures was achieved efficiently without GFs. Enhanced sphere-forming activity was detected in GF-free cultures compared with GF-containing cultures in MCF7, A549 and U87 cells. Analysis of conditioned media (CM) demonstrated an elevated level of EGF secretion in GF-free CM compared with GF-containing CM in MCF7 cells. By contrast, cells in GF-free CM exhibited almost no secretion of bFGF, whereas cells in GF-containing CM secreted a high level of bFGF. The addition of EGF enhanced sphere formation, whereas bFGF suppressed sphere formation under GF-free conditions in MCF7 cells. Conversely, the addition of bFGF suppressed sphere formation in these cells. Notably, bFGF markedly suppressed EGF receptor (EGFR) expression and EGF secretion in MCF7 and A549 cells. Consistent with this result, EGFR blockade with pharmacological inhibitors significantly suppressed sphere formation in MCF7 and A549 cells under GF-free conditions. Furthermore, the neutralization of EGF also inhibited sphere formation, whereas bFGF neutralization still enhanced sphere formation under these conditions. Together, CSCs may be maintained in a serum-free culture condition without GFs, possibly through autocrine secretion of GFs such as EGF, and the addition of bFGF may not be sufficient for the enrichment of stem-like cancer cells.
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Copy and paste a formatted citation
Spandidos Publications style
Kim J, Jung J, Lee S, Lee J and Park M: Cancer stem-like cells persist in established cell lines through autocrine activation of EGFR signaling. Oncol Lett 3: 607-612, 2012.
APA
Kim, J., Jung, J., Lee, S., Lee, J., & Park, M. (2012). Cancer stem-like cells persist in established cell lines through autocrine activation of EGFR signaling. Oncology Letters, 3, 607-612. https://doi.org/10.3892/ol.2011.531
MLA
Kim, J., Jung, J., Lee, S., Lee, J., Park, M."Cancer stem-like cells persist in established cell lines through autocrine activation of EGFR signaling". Oncology Letters 3.3 (2012): 607-612.
Chicago
Kim, J., Jung, J., Lee, S., Lee, J., Park, M."Cancer stem-like cells persist in established cell lines through autocrine activation of EGFR signaling". Oncology Letters 3, no. 3 (2012): 607-612. https://doi.org/10.3892/ol.2011.531
Copy and paste a formatted citation
x
Spandidos Publications style
Kim J, Jung J, Lee S, Lee J and Park M: Cancer stem-like cells persist in established cell lines through autocrine activation of EGFR signaling. Oncol Lett 3: 607-612, 2012.
APA
Kim, J., Jung, J., Lee, S., Lee, J., & Park, M. (2012). Cancer stem-like cells persist in established cell lines through autocrine activation of EGFR signaling. Oncology Letters, 3, 607-612. https://doi.org/10.3892/ol.2011.531
MLA
Kim, J., Jung, J., Lee, S., Lee, J., Park, M."Cancer stem-like cells persist in established cell lines through autocrine activation of EGFR signaling". Oncology Letters 3.3 (2012): 607-612.
Chicago
Kim, J., Jung, J., Lee, S., Lee, J., Park, M."Cancer stem-like cells persist in established cell lines through autocrine activation of EGFR signaling". Oncology Letters 3, no. 3 (2012): 607-612. https://doi.org/10.3892/ol.2011.531
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