Anticancer potential of an ethanol extract of Asiasari radix against HCT-116 human colon cancer cells in vitro

Oh,Se-Mi; Kim,Jinhee; Lee,Jun; Yi,Jin-Mu; Oh,Dal-Seok; Bang,Ok-Sun; Kim,No  Soo

doi:10.3892/ol.2012.1012

January 2013 Volume 5 Issue 1

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January 2013 Volume 5 Issue 1

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Article

Anticancer potential of an ethanol extract of Asiasari radix against HCT-116 human colon cancer cells in vitro

Authors:
- Se-Mi Oh
- Jinhee Kim
- Jun Lee
- Jin-Mu Yi
- Dal-Seok Oh
- Ok-Sun Bang
- No Soo Kim
View Affiliations / Copyright

Affiliations: KM-Based Herbal Drug Research Group, Korea Institute of Oriental Medicine, Daejeon 305-811, Republic of Korea
Pages: 305-310
|
Published online on: November 1, 2012

https://doi.org/10.3892/ol.2012.1012
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Abstract

Radix of Asiasarum heterotropoides var. mandshuricum F. Maekawa (A. radix) has been prescribed for treating pain, allergies and inflammatory disorders in traditional oriental medicine. However, only limited information on the anticancer effects of A. radix is currently available. The aim of this study was to determine the anticancer effect of the ethanol extract of A. radix (EEAR) on HCT-116 human colon cancer cells and to investigate its underlying mechanisms of action. EEAR significantly induced G2/M cell cycle arrest and apoptosis in HCT-116 cells. EEAR-induced apoptosis was observed in parallel with activation of caspases and an increased ratio of Bax (pro-apoptotic)/Bcl2 (anti-apoptotic). Western blot analyses revealed that EEAR elevated the expression of p53 and p21Waf/Cip1 and decreased the expression of the regulator proteins of G2/M phase progression, such as cdc2 and cyclin B. The upregulation of p53 by EEAR was due to the increased levels of p53 mRNA without a similar increase in proteasome-mediated p53 degradation. EEAR-induced apoptosis in HCT-116 cells was dependent on p53 expression, as determined by siRNA-mediated p53 knockdown. Taken together, these results suggest that EEAR inhibits the growth of the HCT-116 cells through induction of G2/M cell cycle arrest and apoptosis, which are mediated by p53 expression.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Anticancer potential of an ethanol extract of Asiasari radix against HCT-116 human colon cancer cells in vitro

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