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Article

Expression and localization of TASK-1, -2 and -3 channels in MG63 human osteoblast-like cells

  • Authors:
    • Xiantao Li
    • Xuehai Dong
    • Shouchao Zheng
    • Jun Xiao
  • View Affiliations / Copyright

    Affiliations: College of Biomedical Engineering, South-Central University for Nationalities, Wuhan 430074, P.R. China, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, P.R. China
  • Pages: 865-869
    |
    Published online on: December 20, 2012
       https://doi.org/10.3892/ol.2012.1088
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Abstract

It is well known that a number of ion channels are involved in the proliferation, migration and invasion of tumor cells. TASK channels, an acid-sensitive subgroup of the two‑pore‑domain K+ channel (K2P) family, are expressed in numerous types of tissue and exhibit various physiological functions depending on the cell type. In the present study, we employed RT-PCR and western blot analysis to determine the expression of TASK-1, -2 and -3 at the mRNA and protein levels in MG63 human osteoblast-like cells. Immunofluorescence with specific antibodies against the TASK channels revealed the localization patterns at the plasma membrane and the juxtanuclear compartment. The induced fluctuations in the extracellular pH from 7.4 to 6.9 and to 6.4 significantly reduced the proliferation rate of MG63 cells by 44.3 and 90.1%, respectively. These data revealed the expression of TASK-1, -2 and -3, and the correlation between TASK channels and cell proliferation in MG63 cells, suggesting that these channels may be involved in the tumorigenesis of osteosarcoma.
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Copy and paste a formatted citation
Spandidos Publications style
Li X, Dong X, Zheng S and Xiao J: Expression and localization of TASK-1, -2 and -3 channels in MG63 human osteoblast-like cells. Oncol Lett 5: 865-869, 2013.
APA
Li, X., Dong, X., Zheng, S., & Xiao, J. (2013). Expression and localization of TASK-1, -2 and -3 channels in MG63 human osteoblast-like cells. Oncology Letters, 5, 865-869. https://doi.org/10.3892/ol.2012.1088
MLA
Li, X., Dong, X., Zheng, S., Xiao, J."Expression and localization of TASK-1, -2 and -3 channels in MG63 human osteoblast-like cells". Oncology Letters 5.3 (2013): 865-869.
Chicago
Li, X., Dong, X., Zheng, S., Xiao, J."Expression and localization of TASK-1, -2 and -3 channels in MG63 human osteoblast-like cells". Oncology Letters 5, no. 3 (2013): 865-869. https://doi.org/10.3892/ol.2012.1088
Copy and paste a formatted citation
x
Spandidos Publications style
Li X, Dong X, Zheng S and Xiao J: Expression and localization of TASK-1, -2 and -3 channels in MG63 human osteoblast-like cells. Oncol Lett 5: 865-869, 2013.
APA
Li, X., Dong, X., Zheng, S., & Xiao, J. (2013). Expression and localization of TASK-1, -2 and -3 channels in MG63 human osteoblast-like cells. Oncology Letters, 5, 865-869. https://doi.org/10.3892/ol.2012.1088
MLA
Li, X., Dong, X., Zheng, S., Xiao, J."Expression and localization of TASK-1, -2 and -3 channels in MG63 human osteoblast-like cells". Oncology Letters 5.3 (2013): 865-869.
Chicago
Li, X., Dong, X., Zheng, S., Xiao, J."Expression and localization of TASK-1, -2 and -3 channels in MG63 human osteoblast-like cells". Oncology Letters 5, no. 3 (2013): 865-869. https://doi.org/10.3892/ol.2012.1088
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