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Print ISSN: 1792-1074 Online ISSN: 1792-1082
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May 2012 Volume 3 Issue 5

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International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

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Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

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Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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Article

Predicting chemosensitivity in osteosarcoma prior to chemotherapy: An investigational study of biomarkers with immunohistochemistry

  • Authors:
    • Yong Chen
    • Yun Yang
    • Zhanna Yuan
    • Chunmeng Wang
    • Yingqiang Shi
  • View Affiliations / Copyright

    Affiliations: Department of Surgical Oncology, Fudan University Shanghai Cancer Center, Shanghai 200032, P.R. China, Department of Bone and Soft Tissue Tumors, Tianjin Medical University Cancer Institute and Hospital, Tianjin 300060, P.R. China, Key Laboratory of Cancer Prevention and Treatment, Tianjin 300060, P.R. China
  • Pages: 1011-1016
    |
    Published online on: February 10, 2012
       https://doi.org/10.3892/ol.2012.604
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Abstract

Osteosarcoma has one of the worst prognoses in adolescents; only 20-60% of patients have high rates of histological necrosis with intensive neoadjuvant chemotherapy. In this study, we investigated the prognostic values of hypoxia‑inducible factor 1 α (HIF-1α), apurinic endonuclease 1 (APE1), vascular endothelial growth factor (VEGF) and cycloogenase-2 (COX-2) protein expression and their predictive value of tumor necrosis rate and prognosis, as well as their interrelationships. Formalin-fixed paraffin‑embedded tissue samples were obtained from 49 patients with osteosarcoma. Immunohistochemistry assays were performed in pre-chemotherapy samples to determine HIF-1α, VEGF, APE1 and COX-2 protein expression levels and hematoxylin and eosin staining was performed in post-operative samples to determine the tumor necrosis rate. Univariate and multivariate analyses were used to assess the impact of protein expression on prognosis. HIF-1α was significantly correlated with every protein we tested: VEGF (P=0.032), APE1 (P<0.001) and COX-2 (P<0.001). HIF-1α protein expression had a significant impact on disease-free survival (P=0.006). Expression of HIF-1α had a sensitivity of 64.7% and a specificity of 71.9% for a poor pathological response (<90% tumor necrosis) versus a good pathological response (≥90% tumor necrosis). In conclusion, expression of HIF-1α is a predictor of tumor response to neoadjuvant chemotherapy and outcome in osteosarcoma, and correlates with VEGF, APE1 and COX-2.
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Copy and paste a formatted citation
Spandidos Publications style
Chen Y, Yang Y, Yuan Z, Wang C and Shi Y: Predicting chemosensitivity in osteosarcoma prior to chemotherapy: An investigational study of biomarkers with immunohistochemistry. Oncol Lett 3: 1011-1016, 2012.
APA
Chen, Y., Yang, Y., Yuan, Z., Wang, C., & Shi, Y. (2012). Predicting chemosensitivity in osteosarcoma prior to chemotherapy: An investigational study of biomarkers with immunohistochemistry. Oncology Letters, 3, 1011-1016. https://doi.org/10.3892/ol.2012.604
MLA
Chen, Y., Yang, Y., Yuan, Z., Wang, C., Shi, Y."Predicting chemosensitivity in osteosarcoma prior to chemotherapy: An investigational study of biomarkers with immunohistochemistry". Oncology Letters 3.5 (2012): 1011-1016.
Chicago
Chen, Y., Yang, Y., Yuan, Z., Wang, C., Shi, Y."Predicting chemosensitivity in osteosarcoma prior to chemotherapy: An investigational study of biomarkers with immunohistochemistry". Oncology Letters 3, no. 5 (2012): 1011-1016. https://doi.org/10.3892/ol.2012.604
Copy and paste a formatted citation
x
Spandidos Publications style
Chen Y, Yang Y, Yuan Z, Wang C and Shi Y: Predicting chemosensitivity in osteosarcoma prior to chemotherapy: An investigational study of biomarkers with immunohistochemistry. Oncol Lett 3: 1011-1016, 2012.
APA
Chen, Y., Yang, Y., Yuan, Z., Wang, C., & Shi, Y. (2012). Predicting chemosensitivity in osteosarcoma prior to chemotherapy: An investigational study of biomarkers with immunohistochemistry. Oncology Letters, 3, 1011-1016. https://doi.org/10.3892/ol.2012.604
MLA
Chen, Y., Yang, Y., Yuan, Z., Wang, C., Shi, Y."Predicting chemosensitivity in osteosarcoma prior to chemotherapy: An investigational study of biomarkers with immunohistochemistry". Oncology Letters 3.5 (2012): 1011-1016.
Chicago
Chen, Y., Yang, Y., Yuan, Z., Wang, C., Shi, Y."Predicting chemosensitivity in osteosarcoma prior to chemotherapy: An investigational study of biomarkers with immunohistochemistry". Oncology Letters 3, no. 5 (2012): 1011-1016. https://doi.org/10.3892/ol.2012.604
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