Optimization and apoptosis induction by RNAi with UTMD technology in vitro

  • Authors:
    • Zhi-Yi Chen
    • Kun Liang
    • Xiu-Jie Sheng
    • Bing Si-Tu
    • Xiao-Fang Sun
    • Jian‑Qiao  Liu
    • Ri-Xiang Qiu
    • Hua Zhang
    • Yue-Wei Li
    • Xin-Xin Zhou
    • Jiang-Xiu Yu
  • View Affiliations

  • Published online on: February 15, 2012     https://doi.org/10.3892/ol.2012.610
  • Pages: 1030-1036
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Abstract

Apoptosis induction by short hairpin RNA (shRNA) expression vectors may be an efficient and promising strategy for cancer gene therapy. Ultrasound-targeted microbubble destruction (UTMD) is an appealing technique; however, there few data are available to demonstrate the feasibility and to optimize the methodology for this technology. The aim of this study was to optimize this technique and to elucidate the effects on gene inhibition and apoptosis induction in vitro. Human cervical cancer cell lines were obtained and cultured.shRNA vectors were constructed, and the UTMD technique was examined to determine whether or not it was suitable for shRNA transfection into cells. Cells were then examined using flow cytometry. The results revealed that the optimal irradiation parameters obtained higher transfection efficiency and did not affect the integrity of plasmid DNA. We concluded that survivin downregulation with shRNA expression vectors, mediated by the optimal UTMD parameters, markedly induced cell apoptosis and caused cell cycle arrest, laying a foundation for further investigation of this cancer therapy.
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May 2012
Volume 3 Issue 5

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Chen Z, Liang K, Sheng X, Si-Tu B, Sun X, Liu JQ, Qiu R, Zhang H, Li Y, Zhou X, Zhou X, et al: Optimization and apoptosis induction by RNAi with UTMD technology in vitro. Oncol Lett 3: 1030-1036, 2012
APA
Chen, Z., Liang, K., Sheng, X., Si-Tu, B., Sun, X., Liu, J. ... Yu, J. (2012). Optimization and apoptosis induction by RNAi with UTMD technology in vitro. Oncology Letters, 3, 1030-1036. https://doi.org/10.3892/ol.2012.610
MLA
Chen, Z., Liang, K., Sheng, X., Si-Tu, B., Sun, X., Liu, J., Qiu, R., Zhang, H., Li, Y., Zhou, X., Yu, J."Optimization and apoptosis induction by RNAi with UTMD technology in vitro". Oncology Letters 3.5 (2012): 1030-1036.
Chicago
Chen, Z., Liang, K., Sheng, X., Si-Tu, B., Sun, X., Liu, J., Qiu, R., Zhang, H., Li, Y., Zhou, X., Yu, J."Optimization and apoptosis induction by RNAi with UTMD technology in vitro". Oncology Letters 3, no. 5 (2012): 1030-1036. https://doi.org/10.3892/ol.2012.610